Nitrogen-containing saturated heterocyclic compound

ABSTRACT

The present invention provides a nitrogen-containing saturated heterocyclic compound of the formula [I]: 
     
       
         
         
             
             
         
       
     
     wherein R 1  is a cycloalkyl group and the like, R 22  is an optionally substituted aryl and the like, R is a lower alkyl and the like, T is a carbonyl group, Z is —O— and the like, and R 3  to R 6  are the same or different and a hydrogen atom and the like; or a pharmaceutically acceptable salt, that is useful as a renin inhibitor.

CROSS REFERENCE TO RELATED APPLICATIONS

This application is a Divisional of copending application Ser. No.14/005,512, filed on Sep. 16, 2013, which was filed as PCT InternationalApplication No. PCT/JP2012/056750 on Mar. 15, 2012, which claims thebenefit under 35 U.S.C. §119(a) to Patent Application No. 2011-058338,filed in Japan on Mar. 16, 2011, all of which are hereby expresslyincorporated by reference into the present application.

TECHNICAL FIELD

The present invention relates to nitrogen-containing saturatedheterocyclic compounds which are useful as a medicine, especially as arenin inhibitor, pharmaceutically acceptable salts and intermediatesthereof.

BACKGROUND ART

Renin inhibitors are expected as a medicine for the prevention and/ortreatment of diseases such as hypertension, heart failure, diabeticnephropathy and the like, and 3,4-substituted piperidine derivatives aredisclosed for example (Patent Literature 1). But a morpholine derivativeis not described in the literature.

Also WO 2008/153182 discloses some morpholine derivatives but they arecompounds having a formula I wherein R is a hydrogen atom (PatentLiterature 2).

CITATION LIST Patent Literatures

-   Patent Literature 1: WO 06/069788 (US 2009/0312304A)-   Patent Literature 2: WO 2008/153182 (US 2010/0240644A)

DISCLOSURE OF INVENTION Technical Problem

The present invention provides novel nitrogen-containing saturatedheterocyclic compounds having an excellent activity to inhibit renin.

Solution to Problem

In order to solve the problem, the inventors have extensively studied tofind novel nitrogen-containing saturated heterocyclic compounds havingan excellent activity to inhibit renin and finally completed the presentinvention.

The present invention is as follows;

1. A compound of the formula [I];

wherein R¹ is a cycloalkyl group or a non-substituted alkyl group;R²² is 1) an optionally substituted aryl group, 2) an optionallysubstituted tetrahydronaphthyl group, 3) an optionally substitutednaphthylidinyl group, 4) an optionally substituted pyridyl group, 5) anoptionally substituted pyrazolopyridyl group, 6) an optionallysubstituted indolyl group, 7) an optionally substituted benzofuranylgroup, 8) an optionally substituted benzothienyl group, 9) an optionallysubstituted quinolyl group, 10) an optionally substituted cromanylgroup, 11) an optionally substituted dihydrobenzofuranyl group, 12) anoptionally substituted indazolyl group, 13) an optionally substitutedpyrrolopyridinyl group, 14) an optionally substituted benzoisoxazolylgroup, 15) an optionally substituted xanthenyl group, 16) an optionallysubstituted indolinyl group, 17) an optionally substituted quinazolinylgroup, 18) an optionally substituted dihydroquinazolinyl group, 19) anoptionally substituted furopyridyl group, 20) an optionally substituteddihydrofuropyridyl group, 21) an optionally substituted quinoxalinylgroup, 22) an optionally substituted thienopyridyl group, 23) anoptionally substituted dihydropyranopyridyl group, 24) an optionallysubstituted dihydrobenzothienyl group, 25) an optionally substituteddihydrothienopyridyl group, or 26) an optionally substitutedimidazopyridinyl group;R is a lower alkyl group or forms a ring by linking with R²² on eachterminal;T is a carbonyl group;Z is —O—, —NH— or a single bond;R³, R⁴, R⁵ and R⁶ are the same or different, a hydrogen atom, anoptionally substituted carbamoyl group or an optionally substitutedalkyl group;or a pharmaceutically acceptable salt thereof,2. A compound of the formula [II]

wherein R¹ is a cycloalkyl group or a non-substituted alkyl group;R²² is 1) an optionally substituted aryl group, 2) an optionallysubstituted tetrahydronaphthyl group, 3) an optionally substitutednaphthylidinyl group, 4) an optionally substituted pyridyl group, 5) anoptionally substituted pyrazolopyridyl group, 6) an optionallysubstituted indolyl group, 7) an optionally substituted benzofuranylgroup, 8) an optionally substituted benzothienyl group, 9) an optionallysubstituted quinolyl group, 10) an optionally substituted cromanylgroup, 11) an optionally substituted dihydrobenzofuranyl group, 12) anoptionally substituted indazolyl group, 13) an optionally substitutedpyrrolopyridinyl group, 14) an optionally substituted benzoisoxazolylgroup, 15) an optionally substituted xanthenyl group, 16) an optionallysubstituted indolinyl group, 17) an optionally substituted quinazolinylgroup, 18) an optionally substituted dihydoquinazolinyl group, 19) anoptionally substituted furopyridyl group, 20) an optionally substituteddihydrofuropyridyl group, 21) an optionally substituted quinoxalinylgroup, 22) an optionally substituted thienopyridyl group, 23) anoptionally substituted dihydopyranopyridyl group, 24) an optionallysubstituted dihydrobenzothienyl group, 25) an optionally substituteddihydrothienopyridyl group, or 26) an optionally substitutedimidazopyridinyl group;R is a lower alkyl group or forms a ring by linking with R²² on eachterminal;T is a carbonyl group;Z¹ is —O—, —NP²— or a single bond;R³, R⁴, R⁵ and R⁶ are the same or different, a hydrogen atom, anoptionally substitutedcarbamoyl group or an optionally substituted alkyl group;P¹ is a protecting group and P² is a protecting group;or a salt thereof.

The compound [I] of the present invention is explained in detail below.

The term “alkyl group” or “alkoxy group” in the present invention isexemplified by a straight or branched chain group having 1 to 10 carbonatoms, and groups having 1 to 6 carbon atoms are preferable, and groupshaving 1 to 4 carbon atoms are especially preferable. The term “loweralkyl group” or “lower alkoxy group” is exemplified by a straight orbranched chain group having 1 to 6 carbon atoms, and groups having 1 to4 carbon atoms are preferable.

The term “lower alkanoyl” is exemplified by a straight or branched chaingroup having 2 to 7 carbon atoms, and groups having 2 to 5 carbon atomsare preferable.

The term “cycloalkyl group” is exemplified by a cycloalkyl group having3 to 8 carbon atoms, groups having 3 to 6 carbon atoms are preferableand groups having 3 to 4 carbon atoms are especially preferable.

The term “halogen atom” means a fluorine atom, a chlorine atom, abromine atom and an iodine atom, and a fluorine atom, a chlorine atomand a bromine atom are especially preferable.

The term “an aryl group” is exemplified by a phenyl group, a naphthylgroup and the like.

An example of substituents of the optionally substituted alkyl group inR³ to R⁶ includes a hydroxyl group, an optionally substituted alkoxygroup, a carboxyl group, an optionally substituted carbamoyl group andthe like.

An example of substituents of the optionally substituted aryl group, theoptionally substituted tetrahydronaphthyl group, the optionallysubstituted naphthylidinyl group, the optionally substituted pyridylgroup, the optionally substituted pyrazolopyridyl group, the optionallysubstituted indolyl group, the optionally substituted benzofuranylgroup, the optionally substituted benzothienyl group, the optionallysubstituted quinolyl group, the optionally substituted cromanyl group,the optionally substituted dihydrobenzofuranyl group, the optionallysubstituted indazolyl group, the optionally substituted pyrrolopyridinylgroup, the optionally substituted benzoisoxazolyl group, the optionallysubstituted xanthenyl group, the optionally substituted indolinyl group,the optionally substituted quinazolinyl group, the optionallysubstituted dihydoquinazolinyl group, the optionally substitutedfulopyridyl group, the optionally substituted dihydrofulopyridyl group,the optionally substituted quinoxalinyl group, the optionallysubstituted thienopyridyl group, the optionally substituteddihydopyranopyridyl group, the optionally substituteddihydrobenzothienyl group, the optionally substituteddihydrothienopyridyl group and the optionally substitutedimidazopyridinyl group includes an alkoxy group, an alkoxy groupsubstituted with an alkoxy group, an alkoxy group substituted with analkylcarbonylamino group, an alkoxy group substituted with anarylcarbonylamino group, an alkoxy group substituted with aheterocyclo-substituted carbonylamino group, an alkoxy group substitutedwith a cycloalkylcarbonylamino group, an alkoxy group substituted withan alkoxycarbonylamino group, an alkyl group substituted with analkoxycarbonylamino group, an alkyl group substituted with an alkoxygroup substituted with an alkoxy group, an alkoxy group substituted withan aryl group, a hydroxyl group, an alkyl group, an alkyl groupsubstituted with an alkoxy group, an oxo group, a halogen atom, analkoxy group substituted with a halogen atom, an alkyl group substitutedwith a halogen atom, an aryloxy group, an aryl group, an aryl groupsubstituted with an alkoxy group, a heterocyclic group, a cyano group, alower alkanoyl group, a carbamoyl group, a carbamoyl group substitutedwith an alkyl group and the like.

An example of substituents of the optionally substituted alkoxy groupincludes a hydroxyl group, an alkoxy group, a halogen atom, an alkoxygroup substituted with a halogen atom, an amino group substituted withan alkylcarbonyl group, an amino group substituted with an arylcarbonylgroup, a carbonylamino group substituted with a heterocyclo group, anamino group substituted with a cycloalkylcarbonyl, an amino groupsubstituted with an alkoxycarbonyl group, an aryl group, an aryloxygroup and the like.

An example of substituents of the optionally substituted carbamoyl groupincludes an alkyl group, an alkyl group substituted with a hydroxylgroup, an alkyl group substituted with an alkoxy group, an alkyl groupsubstituted with a phenyl group, a cycloalkyl group, a pyrrolidinylgroup optionally substituted with a hydroxyalkyl group or analkoxy-substituted alkyl group and the like.

An example of the heterocyclic group includes a pyridyl group, apyrimidyl group, a furyl group, a thienyl group, a quinolyl group, atetrahydroquinolyl group, a isoquinolyl group, a tetrahydroisoquinolylgroup, an indolyl group, an indolinyl group, an indazolyl group, abenzofuranyl group, a benzothienyl group, a dihydrobenzofuranyl group,dihydrocromenyl group, a pyrrolopyridyl group, a benzoxadinyl group, apyrazolyl group and the like.

The present invention includes the following inventions:

(a1) a compound [I] wherein R²² is a group selected from

1) an optionally substituted aryl group,

2) an optionally substituted tetrahydronaphthyl group,

3) an optionally substituted naphthylidinyl group,

4) an optionally substituted pyridyl group,

5) an optionally substituted pyrazolopyridyl group,

6) an optionally substituted indolyl group,

7) an optionally substituted benzofuranyl group,

8) an optionally substituted benzothienyl group,

9) an optionally substituted quinolyl group,

10) an optionally substituted cromanyl group,

11) an optionally substituted dihydrobenzofuranyl group,

12) an optionally substituted indazolyl group,

13) an optionally substituted pyrrolopyridinyl group,

14) an optionally substituted benzoisoxazolyl group,

15) an optionally substituted xanthenyl group,

16) an optionally substituted indolinyl group,

17) an optionally substituted quinazolinyl group,

18) an optionally substituted dihydoquinazolinyl group,

19) an optionally substituted furopyridyl group,

20) an optionally substituted dihydrofuropyridyl group,

21) an optionally substituted quinoxalinyl group,

22) an optionally substituted thienopyridyl group,

23) an optionally substituted dihydopyranopyridyl group,

24) an optionally substituted dihydrobenzothienyl group,

25) an optionally substituted dihydrothienopyridyl group, and

26) an optionally substituted imidazopyridinyl group;

R¹ is a cyclopropyl group;

Z is —O— or —NH—; and

R³, R⁴, R⁵ and R⁶ are a hydrogen atom;or a pharmaceutically acceptable salt thereof,(a2) a compound wherein R²² is a group selected from

1) an optionally substituted phenyl group,

2) an optionally substituted naphthyl group,

3) an optionally substituted tetrahydronaphthy group,

4) an optionally substituted naphthyldinyl group,

5) an optionally substituted pyridyl group,

6) an optionally substituted pyrazolopyridyl group,

7) an optionally substituted indolyl group,

8) an optionally substituted benzofuranyl group,

9) an optionally substituted benzothienyl group,

10) an optionally substituted quinolyl group,

11) an optionally substituted cromanyl group,

12) an optionally substituted dihydrobenzofuranyl group,

13) an optionally substituted indazolyl group,

14) an optionally substituted pyrrolopyridinyl group,

15) an optionally substituted benzoisoxazolyl group,

16) an optionally substituted xanthenyl group,

17) an optionally substituted indolinyl group,

18) an optionally substituted quinazolinyl group,

19) an optionally substituted dihydoquinazolinyl group,

20) an optionally substituted furopyridyl group,

21) an optionally substituted dihydrofuropyridyl group,

22) an optionally substituted quinoxalinyl group,

23) an optionally substituted thienopyridyl group,

24) an optionally substituted dihydopyranopyridyl group,

25) an optionally substituted dihydrobenzothienyl group,

26) an optionally substituted dihydrothienopyridyl group, and

27) an optionally substituted imidazopyridinyl group;

R¹ is a cyclopropyl group;

Z is —O—; and

R³, R⁴, R⁵ and R⁶ are a hydrogen atom;or a pharmaceutically acceptable salt thereof,(a3) the compound of (a1) or (a2) above wherein R²² is any group of 1)to 27) described below;

1) a phenyl group optionally substituted with the same or different, oneto four group(s) selected from a phenyl lower alkoxy group, a halogenatom, a lower alkyl group, a lower alkyl group substituted with a loweralkoxy group, a lower alkyl group substituted with a loweralkoxycarbonylamino group, a lower alkoxy group substituted with a loweralkoxy group, an aryl group substituted with a lower alkoxy group, aheterocyclic group, a cyano group and a lower alkoxy group,

2) a naphthyl group optionally substituted with the same or different,one to six group(s) selected from a trihalogeno lower alkoxy group, alower alkanoylamino lower alkoxy group, a halogen atom, a lower alkylgroup substituted with a lower alkoxy group, a lower alkyl groupsubstituted with a lower alkoxycarbonylamino group, a lower alkyl group,a lower alkoxy group substituted with a lower alkoxy group, an arylgroup, an aryl group substituted with a lower alkoxy group, aheterocyclic group, a cyano group and a lower alkoxy group,

3) a tetrahydronaphthyl group optionally substituted with the same ordifferent, one to six group(s) selected from a halogen atom, a loweralkyl group substituted with a lower alkoxy group, a lower alkyl groupsubstituted with a lower alkoxycarbonylamino group, a lower alkyl group,a lower alkoxy group substituted with a lower alkoxy group, an arylgroup substituted with a lower alkoxy group, a heterocyclic group, acyano group and a lower alkoxy group,

4) a naphthylidinyl group optionally substituted with the same ordifferent, one to five group(s) selected from a halogen atom, a loweralkyl group substituted with a lower alkoxy group, a lower alkyl groupsubstituted with a lower alkoxycarbonylamino group, a lower alkyl group,a lower alkoxy group substituted with a lower alkoxy group, an arylgroup substituted with a lower alkoxy group, a heterocyclic group, acyano group and a lower alkoxy group,

5) a pyridyl group optionally substituted with the same or different,one to four group(s) selected from a halogen atom, a lower alkyl groupsubstituted with a lower alkoxy group, a lower alkyl group substitutedwith a lower alkoxycarbonylamino group, a lower alkyl group, a loweralkoxy group substituted with a lower alkoxy group, an aryl groupsubstituted with a lower alkoxy group, a heterocyclic group, a cyanogroup and a lower alkoxy group,

6) a pyrazolopyridyl group optionally substituted with the same ordifferent, one to four group(s) selected from a halogen atom, a loweralkyl group substituted with a lower alkoxy group, a lower alkyl groupsubstituted with a lower alkoxycarbonylamino group, a lower alkyl group,a lower alkoxy group substituted with a lower alkoxy group, an arylgroup substituted with a lower alkoxy group, a heterocyclic group, acyano group and a lower alkoxy group,

7) an indolyl group optionally substituted with the same or different,one to five group(s) selected from a halogen atom, a lower alkyl groupsubstituted with a lower alkoxy group, a lower alkyl group substitutedwith a lower alkoxycarbonylamino group, a lower alkyl group, a loweralkoxy group substituted with a lower alkoxy group, an aryl groupsubstituted with a lower alkoxy group, a heterocyclic group, a cyanogroup and a lower alkoxy group,

8) a benzofuranyl group optionally substituted with the same ordifferent, one to four group(s) selected from a halogen atom, a loweralkyl group substituted with a lower alkoxy group, a lower alkyl groupsubstituted with a lower alkoxycarbonylamino group, a lower alkyl group,a lower alkoxy group substituted with a lower alkoxy group, an arylgroup substituted with a lower alkoxy group, a heterocyclic group, acyano group and a lower alkoxy group,

9) a benzothienyl group optionally substituted with the same ordifferent, one to four group(s) selected from a halogen atom, a loweralkyl group substituted with a lower alkoxy group, a lower alkyl groupsubstituted with a lower alkoxycarbonylamino group, a lower alkyl group,a lower alkoxy group substituted with a lower alkoxy group, an arylgroup substituted with a lower alkoxy group, a heterocyclic group, acyano group and a lower alkoxy group,

10) a quinolyl group optionally substituted with the same or different,one to four group(s) selected from a halogen atom, a lower alkyl groupsubstituted with a lower alkoxy group, a lower alkyl group substitutedwith a lower alkoxycarbonylamino group, a lower alkyl group, a loweralkoxy group substituted with a lower alkoxy group, an aryl groupsubstituted with a lower alkoxy group, a heterocyclic group, a cyanogroup and a lower alkoxy group,

11) a cromanyl group optionally substituted with the same or different,one to five group(s) selected from a halogen atom, a lower alkyl groupsubstituted with a lower alkoxy group, a lower alkyl group substitutedwith a lower alkoxycarbonylamino group, a lower alkyl group, a loweralkoxy group substituted with a lower alkoxy group, an aryl groupsubstituted with a lower alkoxy group, a heterocyclic group, a cyanogroup and a lower alkoxy group,

12) a dihydrobenzofuranyl group optionally substituted with the same ordifferent, one to four group(s) selected from a halogen atom, a loweralkyl group substituted with a lower alkoxy group, a lower alkyl groupsubstituted with a lower alkoxycarbonylamino group, a lower alkyl group,a lower alkoxy group substituted with a lower alkoxy group, an arylgroup substituted with a lower alkoxy group, a heterocyclic group, acyano group and a lower alkoxy group,

13) an indazolyl group optionally substituted with the same ordifferent, one to five group(s) selected from a halogen atom, a loweralkyl group substituted with a lower alkoxy group, a lower alkyl groupsubstituted with a lower alkoxycarbonylamino group, a lower alkyl group,a lower alkoxy group substituted with a lower alkoxy group, an arylgroup substituted with a lower alkoxy group, a heterocyclic group, acyano group and a lower alkoxy group,

14) a pyrrolopyridinyl group optionally substituted with the same ordifferent, one to three group(s) selected from a halogen atom, a loweralkyl group substituted with a lower alkoxy group, a lower alkyl groupsubstituted with a lower alkoxycarbonylamino group, a lower alkyl group,a lower alkoxy group substituted with a lower alkoxy group, an arylgroup substituted with a lower alkoxy group, a heterocyclic group, acyano group and a lower alkoxy group,

15) a benzoisoxazolyl group optionally substituted with the same ordifferent, one to four group(s) selected from a halogen atom, a loweralkyl group substituted with a lower alkoxy group, a lower alkyl groupsubstituted with a lower alkoxycarbonylamino group, a lower alkyl group,a lower alkoxy group substituted with a lower alkoxy group, an arylgroup substituted with a lower alkoxy group, a heterocyclic group, acyano group and a lower alkoxy group,

16) a xanthenyl group optionally substituted with the same or different,one to six group(s) selected from a halogen atom, a lower alkyl groupsubstituted with a lower alkoxy group, a lower alkyl group substitutedwith a lower alkoxycarbonylamino group, a lower alkyl group, a loweralkoxy group substituted with a lower alkoxy group, an aryl groupsubstituted with a lower alkoxy group, a heterocyclic group, a cyanogroup and a lower alkoxy group,

17) an indolinyl group optionally substituted with the same ordifferent, one to five group(s) selected from a halogen atom, a loweralkyl group substituted with a lower alkoxy group, a lower alkyl groupsubstituted with a lower alkoxycarbonylamino group, a lower alkyl group,a lower alkoxy group substituted with a lower alkoxy group, an arylgroup substituted with a lower alkoxy group, a heterocyclic group, acyano group and a lower alkoxy group,

18) a quinazolyl group optionally substituted with the same ordifferent, one to five group(s) selected from a halogen atom, a loweralkyl group substituted with a lower alkoxy group, a lower alkyl groupsubstituted with a lower alkoxycarbonylamino group, a lower alkyl group,a lower alkoxy group substituted with a lower alkoxy group, an arylgroup substituted with a lower alkoxy group, a heterocyclic group, acyano group and a lower alkoxy group,

19) a dihydroquinazolinyl group optionally substituted with the same ordifferent, one to five group(s) selected from a halogen atom, a loweralkyl group substituted with a lower alkoxy group, a lower alkyl groupsubstituted with a lower alkoxycarbonylamino group, a lower alkyl group,a lower alkoxy group substituted with a lower alkoxy group, an arylgroup substituted with a lower alkoxy group, a heterocyclic group, acyano group and a lower alkoxy group,

20) a furopyridyl group optionally substituted with the same ordifferent, one to four group(s) selected from a halogen atom, a loweralkyl group substituted with a lower alkoxy group, a lower alkyl groupsubstituted with a lower alkoxycarbonylamino group, a lower alkyl group,a lower alkoxy group substituted with a lower alkoxy group, an arylgroup substituted with a lower alkoxy group, a heterocyclic group, acyano group and a lower alkoxy group,

21) a dihydrofuropyridyl group optionally substituted with the same ordifferent, one to four group(s) selected from a halogen atom, a loweralkyl group substituted with a lower alkoxy group, a lower alkyl groupsubstituted with a lower alkoxycarbonylamino group, a lower alkyl group,a lower alkoxy group substituted with a lower alkoxy group, an arylgroup substituted with a lower alkoxy group, a heterocyclic group, acyano group and a lower alkoxy group,

22) a quinoxalinyl group optionally substituted with the same ordifferent, one to five group(s) selected from a halogen atom, a loweralkyl group substituted with a lower alkoxy group, a lower alkyl groupsubstituted with a lower alkoxycarbonylamino group, a lower alkyl group,a lower alkoxy group substituted with a lower alkoxy group, an arylgroup substituted with a lower alkoxy group, a heterocyclic group, acyano group and a lower alkoxy group,

23) a thienopyridyl group optionally substituted with the same ordifferent, one to four group(s) selected from a halogen atom, a loweralkyl group substituted with a lower alkoxy group, a lower alkyl groupsubstituted with a lower alkoxycarbonylamino group, a lower alkyl group,a lower alkoxy group substituted with a lower alkoxy group, an arylgroup substituted with a lower alkoxy group, a heterocyclic group, acyano group and a lower alkoxy group,

24) a dihydropyranopyridyl group optionally substituted with the same ordifferent, one to four group(s) selected from a halogen atom, a loweralkyl group substituted with a lower alkoxy group, a lower alkyl groupsubstituted with a lower alkoxycarbonylamino group, a lower alkyl group,a lower alkoxy group substituted with a lower alkoxy group, an arylgroup substituted with a lower alkoxy group, a heterocyclic group, acyano group and a lower alkoxy group,

25) a dihydrobenzothienyl group optionally substituted with the same ordifferent, one to four group(s) selected from a halogen atom, a loweralkyl group substituted with a lower alkoxy group, a lower alkyl groupsubstituted with a lower alkoxycarbonylamino group, a lower alkyl group,a lower alkoxy group substituted with a lower alkoxy group, an arylgroup substituted with a lower alkoxy group, a heterocyclic group, acyano group and a lower alkoxy group,

26) a dihydrothienopyridyl group optionally substituted with the same ordifferent, one to four group(s) selected from a halogen atom, a loweralkyl group substituted with a lower alkoxy group, a lower alkyl groupsubstituted with a lower alkoxycarbonylamino group, a lower alkyl group,a lower alkoxy group substituted with a lower alkoxy group, an arylgroup substituted with a lower alkoxy group, a heterocyclic group, acyano group and a lower alkoxy group, and

27) an imidazopyridinyl group optionally substituted with the same ordifferent, one to four group(s) selected from a halogen atom, a loweralkyl group substituted with a lower alkoxy group, a lower alkyl groupsubstituted with a lower alkoxycarbonylamino group, a lower alkyl group,a lower alkoxy group substituted with a lower alkoxy group, an arylgroup substituted with a lower alkoxy group, a heterocyclic group, acyano group and a lower alkoxy group,

or a pharmaceutically acceptable salt thereof(a4) the compound of (a1) or (a2) above wherein R²² is any group of 1)to 13) described below;

1) a phenyl group optionally substituted with the same or different, twoor three groups selected from a phenyl lower alkoxy group, a halogenatom, a lower alkyl group substituted with a lower alkoxy group, a loweralkyl group substituted with a lower alkoxycarbonylamino group, a loweralkyl group, a lower alkoxy group substituted with a lower alkoxy groupand a lower alkoxy group,

2) a naphthyl group optionally substituted with the same or different,one to three group(s) selected from a trihalogeno lower alkoxy group, alower alkanoylamino lower alkoxy group, a halogen atom, an aryl group,an aryl group substituted with a lower alkoxy group, a heterocyclicgroup, a lower alkyl group and a lower alkoxy group substituted with alower alkoxy group,

3) a tetrahydronaphthyl group optionally substituted with one or twogroup(s) selected from a halogen atom and a lower alkoxy groupsubstituted with a lower alkoxy group,

4) an indolyl group optionally substituted with the same or different,one to three group(s) selected from a halogen atom, a cyano group, alower alkoxy group, a lower alkoxy group substituted with an aryl groupgroup, a lower alkyl group and a lower alkyl group substituted with alower alkoxy group,

5) a benzofuranyl group optionally substituted with one or two group(s)selected from a halogen atom and a lower alkoxy group substituted with alower alkoxy group,

6) a benzothienyl group optionally substituted with a lower alkoxy groupsubstituted with a lower alkoxy group,

7) a quinolyl group optionally substituted with a lower alkoxy groupsubstituted with a lower alkoxy group,

8) a cromanyl group optionally substituted with a lower alkoxy groupsubstituted with a lower alkoxy group,

9) a dihydrobenzofuranyl group optionally substituted with one or twogroup(s) selected from a halogen atom and a lower alkoxy groupsubstituted with a lower alkoxy group,

10) an indazolyl group optionally substituted with one or two group(s)selected from a halogen atom and a lower alkoxy group substituted with alower alkoxy group,

11) a pyrrolopyridinyl group optionally substituted with one to threegroup(s) selected from a halogen atom, a lower alkyl group and a loweralkyl group substituted with a lower alkoxy group,

12) a pyrazolopyridyl group optionally substituted with one or twogroup(s) selected from a lower alkyl group substituted with a loweralkoxycarbnylamino group and a lower alkyl group, and

13) a pyridyl group optionally substituted with one or two group(s)selected from a lower alkoxy group, a lower alkyl group and a loweralkyl group substituted with a lower alkoxycarbnylamino group,

or a pharmaceutically acceptable salt thereof(a5) the compound of (a1) or (a2) above wherein R²² is any group of 1)to 13) described below;

1) a phenyl group optionally substituted with two or three groupsselected from a phenyl lower alkoxy group, a fluorine atom, a bromineatom, a chlorine atom, a lower alkyl group substituted with a loweralkoxy group, a lower alkyl group substituted with a loweralkoxycarbonylamino group, a lower alkyl group, a lower alkoxy groupsubstituted with a lower alkoxy group and a lower alkoxy group, a phenylgroup,

2) a naphthyl group optionally substituted with the same or different,one to three group(s) selected from a trihalogeno lower alkoxy group, alower alkanoylamino lower alkoxy group, a fluorine atom, a bromine atom,a chlorine atom, a phenyl group, a phenyl group substituted with a loweralkoxy group, a pyridyl group, a furyl group, a thienyl group, a loweralkyl group and a lower alkoxy group substituted with a lower alkoxygroup,

3) a tetrahydronaphthyl group optionally substituted with one or twogroup(s) selected from a fluorine atom, a bromine atom, a chlorine atomand a lower alkoxy group substituted with a lower alkoxy group,

4) an indolyl group optionally substituted with one to three group(s)selected from a fluorine atom, a bromine atom, a chlorine atom, a cyanogroup, a lower alkoxy group, a lower alkoxy group substituted with aphenyl group, a lower alkyl group and a lower alkyl group substitutedwith a lower alkoxy group,

5) a benzofuranyl group optionally substituted with one or two group(s)selected from a bromine atom, a chlorine atom, and a lower alkoxy groupsubstituted with a lower alkoxy group,

6) a benzothienyl methyl group optionally substituted with a loweralkoxy group substituted with a lower alkoxy group,

7) a quinolyl group optionally substituted with a lower alkoxy groupsubstituted with a lower alkoxy group,

8) a cromanyl group optionally substituted with a lower alkoxy groupsubstituted with a lower alkoxy group,

9) a dihydrobenzofuranyl group optionally substituted with one or twogroup(s) selected from a bromine atom, a chlorine atom, and a loweralkoxy group substituted with a lower alkoxy group,

10) an indazolyl group optionally substituted with one or two group(s)selected from a fluorine atom, a bromine atom, a chlorine atom and alower alkyl group substituted with a lower alkoxy group,

11) a pyrrolopyridinyl group optionally substituted with one or twogroup(s) selected from a fluorine atom, a bromine atom, a chlorine atom,a lower alkyl group and a lower alkyl group substituted with a loweralkoxy group,

12) a pyrazolopyridyl group optionally substituted with one or twogroup(s) selected from a lower alkyl group substituted with a loweralkoxycarbnylamino group and a lower alkyl group, and

13) a pyridyl group optionally substituted with one or two group(s)selected from a lower alkoxy group, a lower alkyl group and a loweralkyl group substituted with a lower alkoxycarbnylamino group,

or a pharmaceutically acceptable salt thereof.(a6) the compound of (a1) or (a2) above wherein R²² is any group of 1)to 13) described below;

1) a phenyl group optionally substituted with two or three groupsselected from a methoxy group substituted with a phenyl group, an ethoxygroup substituted with a phenyl group, a fluorine atom, a bromine atom,a chlorine atom, a methyl group, a propoxy group substituted with amethoxy group, a butyl group substituted with a methoxy group, amethoxycarbonylaminopropyl group and a methoxy group,

2) a naphthyl group optionally substituted with the same or different,one to three group(s) selected from trifluorobutoxy group, anacetylaminoethoxy group, a fluorine atom, a bromine atom, a chlorineatom, a phenyl group, a phenyl group substituted with a methoxy group, apyridyl group, a furyl group, a thienyl group, a methyl group and apropoxy group substituted with a methoxy group,

3) a tetrahydronaphthyl group optionally substituted with one or twogroup(s) selected from a bromine atom, a chlorine atom and a propoxygroup substituted with a methoxy group,

4) an indolyl group optionally substituted with one or two group(s)selected from a fluorine atom, a bromine atom, a chlorine atom, a cyanogroup, a methoxy group, a methoxy group substituted with a phenyl group,a methyl group and a propyl group substituted with a methoxy group,

5) a benzofuranyl group optionally substituted with one or two group(s)selected from a bromine atom, a chlorine atom, and a propoxy groupsubstituted with a methoxy group,

6) a benzothienyl group optionally substituted with a propoxy groupsubstituted with a methoxy group,

7) a quinolyl group optionally substituted with a propoxy groupsubstituted with a methoxy group,

8) a cromanyl group optionally substituted with a propoxy groupsubstituted with a methoxy group,

9) a dihydrobenzofuranyl group optionally substituted with one or twogroup(s) selected from a bromine atom, a chlorine atom, and a propoxygroup substituted with a methoxy group,

10) an indazolyl group optionally substituted with one or two group(s)selected from a fluorine atom, a chlorine atom and a propyl groupsubstituted with a methoxy group,

11) a pyrrolopyridinyl group optionally substituted with one or twogroup(s) selected from a bromine atom, a chlorine atom, a methyl group,an ethyl group, a propyl group substituted with a methoxy group

12) a pyrazolopyridyl group optionally substituted with one or twogroup(s) selected from a methoxycarbonylaminopropyl group and a methylgroup, and

13) a pyridyl group optionally substituted with one or two group(s)selected from a methoxy group, an ethyl group and amethoxycarbonylaminopropyl group,

or a pharmaceutically acceptable salt thereof.(a7) the compound of (a1) or (a2) above wherein R²² is any group of 1)to 27) described below;

1) a phenyl group optionally substituted with one to three group(s)selected from a halogen atom, a trihalogeno lower alkyl group, a cyanogroup, a benzyloxy group, a lower alkoxy group, a dihalogeno loweralkoxy group, an aminocarbonyl group, a lower alkylaminocarbonyl group,di(lower alkyl)aminocarbonyl group, a lower alkylaminocarbonyl groupsubstituted with a lower alkoxy group, a lower alkoxy group substitutedwith a lower alkoxy group, a lower alkyl group substituted with a loweralkoxy group, a lower alkyl group substituted with a loweralkoxycarbonylamino group and a lower alkyl group,

2) a naphthyl group optionally substituted with one or two group(s)selected from a lower alkoxy group substituted with a loweralkoxycarbonylamino group and a lower alkoxy group,

3) a tetrahydronaphthyl group optionally substituted with a lower alkoxygroup,

4) a naphthylidinyl group,

5) a pyridyl group optionally substituted with one or two group(s)selected from a lower alkoxy group, a lower alkyl group and a loweralkyl group substituted with a lower alkoxycarbonylamino group,

6) a pyrazolopyridyl group optionally substituted with one or twogroup(s) selected from a lower alkyl group substituted with a loweralkoxy group, a lower alkyl group substituted with a loweralkoxycarbonylamino group and a lower alkyl group,

7) an indolyl group optionally substituted with one to three group(s)selected from a halogen atom, a lower alkyl group and a lower alkylgroup substituted with a lower alkoxy group,

8) a benzofuranyl group optionally substituted with one or two group(s)selected from a lower alkyl group substituted with a lower alkoxy groupand a lower alkoxy group substituted with a lower alkoxy group,

9) a benzothienyl group,

10) a quinolyl group optionally substituted with one or two group(s)selected from a halogen atom and a lower alkoxy group,

11) a cromanyl group,

12) a dihydrobenzofuranyl group optionally substituted with a loweralkoxy group substituted with a lower alkoxy group,

13) an indazolyl group optionally substituted with one to three group(s)selected from a halogen atom, a lower alkoxy group substituted with alower alkoxy group, a lower alkyl group substituted with a loweralkoxycarbonylamino group, a trihalogeno lower alkyl group, a loweralkyl and a lower alkyl group substituted with a lower alkoxy group,

14) a pyrrolopyridinyl group optionally substituted with one to threegroup(s) selected from a halogen atom, a lower alkyl group, a loweralkyl group substituted with a lower alkoxycarbonylamino group and alower alkyl group substituted with a lower alkoxy group,

15) a benzoisoxazolyl group optionally substituted with one or twogroup(s) selected from a lower alkyl group and a lower alkyl groupsubstituted with a lower alkoxy group,

16) a xanthenyl group,

17) an indolinyl group optionally substituted with one or two group(s)selected from a halogen atom and a lower alkyl group substituted with alower alkoxy group,

18) a quinazolyl group optionally substituted with one or two group(s)selected from a hydroxyl group and a lower alkoxy group substituted witha lower alkoxy group,

19) a dihydroquinazolyl group optionally substituted with an oxo group,

20) a furopyridyl group

21) dihydrofuropyridyl group

22) quinoxalinyl group,

23) a thienopyridyl group

24) a dihydropyranopyridyl group,

25) a dihydrobenzothienyl group,

26) a dihydrothienopyridyl group, and

27) an imidazopyridinyl group

or a pharmaceutically acceptable salt thereof.

(a8) the compound of (a1) or (a2) above wherein R²² is any group of 1)to 27) described below;

1) a phenyl group optionally substituted with one to three group(s)selected from a fluorine atom, a bromine atom, a chlorine atom, atrifluoromethyl group, a cyano group, a benzyloxy group, a methoxygroup, an ethoxy group, an isopropoxy group, difluoromethoxy group, anaminocarbonyl group, a methylaminocarbonyl group, adimethylaminocarbonyl group, a methoxyethylaminocarbonyl group, apropoxy group substituted with a methoxy group, a butyl groupsubstituted with a methoxy group, a methoxycarbonylaminopropyl group anda methoxy group;

2) a naphthyl group optionally substituted with one or two group(s)selected from an ethoxy group substituted with a methoxycarbonylaminogroup and a methoxy group

3) a tetrahydronaphthyl group optionally substituted with a methoxygroup

4) a naphthylidinyl group,

5) a pyridyl group optionally substituted with one or two group(s)selected from a methoxy group, an ethyl group and amethoxycarbonylaminopropyl group,

6) a pyrazolopyridyl group optionally substituted with one or twogroup(s) selected from a butyl group substituted with a methoxy group, amethoxycarbonylaminopropyl group, and a methyl group,

7) an indolyl group optionally substituted with one to three group(s)selected from a chlorine atom, a methyl group and a propyl groupsubstituted with a methoxy group,

8) a benzofuranyl group optionally substituted with one or two group(s)selected from a propyl group substituted with a methoxy group and apropoxy group substituted with a methoxy group,

9) a benzothienyl group,

10) a quinolyl group optionally substituted with one or two group(s)selected from a chlorine atom and a methoxy group,

11) a cromanyl group,

12) a dihydrobenzofuranyl group optionally substituted with a propoxygroup substituted with a methoxy group,

13) an indazolyl group optionally substituted with one to three group(s)selected from a fluorine atom, a chlorine atom, a propoxy groupsubstituted with a methoxy group, a propyl group substituted with amethoxycarbonylamino group, a trifluoromethyl group, a methyl group, apropyl group substituted with a methoxy group, a butyl group substitutedwith a methoxy group,

14) a pyrrolopyridinyl group optionally substituted with one to threegroup(s) selected from a fluorine atom, a chlorine atom, a methyl group,an ethyl group, a propyl group substituted with a methoxycarbonylaminogroup, a propyl group substituted with a methoxy group and a butyl groupsubstituted with a methoxy group,

15) a benzoisoxazolyl group optionally substituted with one or twogroup(s) selected from a methyl group and a propyl group substitutedwith a methoxy group

16) a xanthenyl group,

17) an indolinyl group optionally substituted with one or two group(s)selected from a chlorine atom and a propyl group substituted with amethoxy group,

18) a quinazolinyl group optionally substituted with one or two group(s)selected from a hydroxyl group and a propoxy group substituted with amethoxy group,

19) a dihydroquinazolyl group optionally substituted with an oxo group,

20) a furopyridyl group,

21) a dihydrofuropyridyl group,

22) a quinoxalinyl group,

23) a thienopyridyl group,

24) a dihydropyranopyridyl group,

25) a dihydrobenzothienyl group,

26) a dihydrothienopyridyl group, and

27) an imidazopyridinyl group

or a pharmaceutically acceptable salt thereof(a9) the compound of (a1) or (a2) above wherein the indolyl group of R²²is any group of the next formulae:

the benzofuranyl group of R²² is any group of the next formulae:

the benzothienyl group of R²² is any group of the next formulae:

the quinolyl group of R²² is any group of the next formulae:

the naphthyl group of R²² is any group of the next formulae:

the tetrahydronaphthyl group of R²² is any group of the next formulae:

the cromanyl group of R²² is any group of the next formulae:

the dihydrobenzofuranyl group of R²² is any group of the next formulae:

the indazolyl group of R²² is any group of the next formulae:

the pyrrolopyridinyl group of R²² is any group of the next formulae:

the naphthylidinyl group of R²² is any group of the next formulae:

the benzoisoxazolyl group of R²² is any group of the next formulae:

the xanthenyl group of R²² is a group of the next formula:

the indolinyl group of R²² is a group of the next formula:

the quinazolinyl group of R²² is a group of the next formula:

the quinoxalinyl group of R²² is a group of the next formula:

the furopyridyl group of R²² is any group of the next formulae:

the dihydrofuropyridyl group of R²² is any group of the next formulae:

the imidazopyridyl group of R²² is any group of the next formulae:

and the pyrazolopyridyl group of R²² is any group of the next formulae:

or a pharmaceutically acceptable salt thereof.(a10) the compound of (a1) or (a2) above which is shown by the formula

or a pharmaceutically acceptable salt thereof.(b1) the compound described in any of (a1) to (a10) above, wherein R²²of the compound [I] is selected from

3) an optionally substituted naphthylidinyl group,

4) an optionally substituted pyridyl group,

5) an optionally substituted pyrazolopyridyl group,

10) an optionally substituted cromanyl group,

17) an optionally substituted quinazolinyl group,

18) an optionally substituted dihydroquinazolinyl group,

19) an optionally substituted furopyridyl group,

20) an optionally substituted dihydrofuropyridyl group,

21) an optionally substituted quinoxalinyl group,

22) an optionally substituted thienopyridyl group,

23) an optionally substituted dihydropyranopyridyl group,

24) an optionally substituted dihydrobenzothienyl group, and

25) an optionally substituted dihydrothienopyridyl group,

or a pharmaceutically acceptable salt thereof.(c1) the compounds of (a1) above, which is shown by the formula[I^(c1)]:

wherein R^(b) is lower alkyl,R^(b1) is cycloalkyl or alkyl,the ring B is selected from

1) an aryl group

2) a tetrahydronaphthyl group,

3) a naphthyldinyl group,

4) a pyridyl group,

5) a pyrazolopyridyl group,

6) an indolyl group,

7) a benzofuranyl group,

8) a benzothienyl group,

9) a quinolyl group

10) a cromanyl group,

11) a dihydrobenzofuranyl group,

12) an indazolyl group

13) a pyrrolopyridyl group,

14) a benzoisoxazolyl group,

15) a xanthenyl group,

16) an indolinyl group,

17) a quinazolinyl group

18) a dihydroquinazolinyl group

19) a furopyridyl group

20) a dihydrofuropyridyl group

21) a quinoxalinyl group,

22) a thienopyridyl group,

23) a dihydropyranopyridyl group,

24) a dihydrobenzothienyl group

25) a dihydrothienothienyl group and

26) an imidazopyridinyl group,

R^(b21) to R^(b23) are the same of different, and a group selectedfrom 1) hydrogen, 2) halogen, 3) alkyl optionally substituted with agroup selected from halogen, alkoxy and alkoxycarbonylamino, 4) alkoxyoptionally substituted with a group selected from alkoxy andalkoxycarbonylamino, 5) cyano, 6) carbamoyl optionally substituted withalkyl and 7) oxo,or a pharmaceutically acceptable salt thereof.(c2) the compound of (c1), which is shown by the formula I^(c2):

wherein each symbol is the same as that of the formula I^(c1) above,or a pharmaceutically acceptable salt thereof.(c3) the compound of (c1) or (c2) above, wherein the ring B is a groupselected from

1) an aryl group

4) a pyridyl group

5) a pyrazolopyridyl group

6) an indolyl group,

7) a benzofuranyl group

9) a quinolyl group

11) a dihydrobenzofuranyl group,

12) an indazolyl group,

13) a pyrrolopyridinyl group,

14) a benzoisoxazolyl group,

16) an indolinyl group,

17) a quinazolinyl group, and

18) a dihydroquinazolinyl group,

or a pharmaceutically acceptable salt thereof(c4) the compound of (c1) or (c2) above, wherein the ring B is a groupselected from

3) a naphthylidinyl group

4) a pyridyl group

5) a pyrazolopyridyl group

10) a cromanyl group,

17) a quinazolinyl group, and

18) a dihydroquinazolinyl group,

19) a furopyridyl group,

20) a dihydrofuropyridyl group

21) a quinoxalinyl group,

22) a thienopyridyl group,

23) a dihydropyranopyridyl group

24) a dihydrobenzothienyl group, and

25) a dihydrothienopyridyl group

or a pharmaceutically acceptable salt thereof(c5) the compound of (c4) wherein the ring B is a group selected from

4) a pyridyl group,

5) a pyrazolopyridyl group,

17) a quinazolinyl group, and

18) a dihydroquinazolinyl group,

or a pharmaceutically acceptable salt thereof(c6) the compound of (c4) wherein the ring B is a group selected from

4) a pyridyl group and

5) a pyrazolopyridyl group,

or a pharmaceutically acceptable salt thereof.(c7) the compound of (c4) wherein the ring B is a group of

5) a pyrazolopyridyl group, preferably 3-pyrazolopyridyl group,

or a pharmaceutically acceptable salt thereof.(c8) the compound of any of (c1) to (c7) wherein R^(b1) is a cycloalkylgroupor a pharmaceutically acceptable salt thereof.(c9) the compound of (c8) wherein R^(b1) is a cyclopropyl groupor a pharmaceutically acceptable salt thereof.(c10) the compound of any of (c1) to (c9) wherein R^(b21) is a groupselected from alkyl optionally substituted with alkoxy andalkoxycarbonylamino, and alkoxy optionally substituted with alkoxy andalkoxycarbamoyl,or a pharmaceutically acceptable salt thereof.(C11) the compound of (c10) wherein R^(b21) is a group selected fromalkyl optionally substituted with alkoxy and alkoxycarbonylamino, andalkoxy optionally substituted with alkoxy and alkoxycarbamoyl,or a pharmaceutically acceptable salt thereof.(c12) the compound of (c10) or (c11) wherein R^(b21) to R^(b23) are thesame or different, and a group selected from hydrogen, alkyl and alkoxy,or a pharmaceutically acceptable salt thereof(d1) the compound of (1) selected from those described in examples,or a pharmaceutically acceptable salt thereof.(d2) the compound of (d1) selected from those IC₅₀ values of which aredescribed in Table 90-92,or a pharmaceutically acceptable salt thereof.(d3) the compound of (d2) selected from those having IC₅₀ values lessthan 100 nM in Tables 90-92,or a pharmaceutically acceptable salt thereof.(d4) the compound of (d2) selected from those having IC₅₀ values lessthan 10 nM in Tables 90-92,or a pharmaceutically acceptable salt thereof.(d5) the compound of any of (d1) to (d5), which is shown by the formulaI^(c2),or a pharmaceutically acceptable salt thereof.(d6) the compound of any of (d1) to (d5), which is selected from (c5),or a pharmaceutically acceptable salt thereof.(d7)N-cyclopropyl-N-{1-[1-(4-methoxybutyl)-1H-pyrazolo[3,4-b]pyridin-3-yl]ethyl}morpholin-2-carboxamide;

-   methyl    (3-{3-[1-{cyclopropyl(morpholin-2-ylcarbonyl)amino}ethyl]-1H-pyrazolo[3,4-b]pyridin-1-yl}propyl)carbamate;-   methyl    (3-{3-[1-{cyclopropyl(morpholin-2-ylcarbonyl)amino}ethyl]-6-methyl-1H-pyrazolo[3,4-b]pyridin-1-yl}propyl)carbamate;-   methyl    (3-{5-[1-{cyclopropyl(morpholin-2-ylcarbonyl)amino}ethyl]-2-methoxy    pyridin-3-yl}propyl)carbamate;-   methyl    (3-{5-[1-{cyclopropyl(morpholin-2-ylcarbonyl)amino}ethyl]-2-ethylpyridin-3-yl}propyl)carbamate;-   methyl    (3-{4-[1-{cyclopropyl(morpholin-2-ylcarbonyl)amino}ethyl]-6-methoxy    pyridin-2-yl}propyl)carbamate;-   methyl    (3-{4-[1-{cyclopropyl(morpholin-2-ylcarbonyl)amino}ethyl]-6-ethyl    pyridin-2-yl}propyl)carbamate;-   methyl    (3-{4-[1-{cyclopropyl(morpholin-2-ylcarbonyl)amino}ethyl]-6-(2-methoxyethoxy)pyridin-2-yl}propyl)carbamate;-   methyl    (3-{4-[1-{cyclopropyl(morpholin-2-ylcarbonyl)amino}ethyl]-6-(3-methoxypropyl)pyridin-2-yl}propyl)carbamate;    or-   methyl    (3-{4-[1-{cyclopropyl(morpholin-2-ylcarbonyl)amino}ethyl]-6-(propan-2-yloxy)pyridin-2-yl}propyl)carbamate,    or a pharmaceutically acceptable salt thereof    (e1) a pharmaceutical composition comprising the compound of any of    (1), (a1) to (a10), (b1), (c1) to (c12) and (d1) to (d7) or a    pharmaceutically acceptable salt thereof.    (e2) a renin inhibitor comprising the compound of any of (1), (a1)    to (a10), (b1), (c1) to (c12) and (d1) to (d7) or a pharmaceutically    acceptable salt thereof    (e3) a pharmaceutical composition for the treatment and/or    prophylaxis of hypertension, cardiac failure, diabeti nephropathy    and the like, comprising the compound of any of (1), (a1) to (a10),    (b1), (c1) to (c12) and (d1) to (d7) or a pharmaceutically    acceptable salt thereof.    (e4) the compound or the pharmaceutically acceptable salt thereof    for use in renin inhibition, described in any of (1), (a1) to (a10),    (b1), (c1) to (c12) and (d1) to (d7).    (e5) the compound or the pharmaceutically acceptable salt thereof    for use in the treatment and/or prophylaxis of diseases resolved by    renin-inhibition, described in any of (1), (a1) to (a10), (b1), (c1)    to (c12) and (d1) to (d7).    (e6) the compound or the pharmaceutically acceptable salt thereof    for use in the treatment and/or prophylaxis of hypertension, cardiac    failure, diabetic nephropathy and the like, described in any of (1),    (a1) to (a10), (b1), (c1) to (c12) and (di) to (d7).    (e7) a method for inhibiting renin, comprising administration of the    compound or the pharmaceutically acceptable salt thereof, described    in any of (1), (a1) to (a10), (b1), (c1) to (c12) and (d1) to (d7).    (e8) a method for the treatment and/or prophylaxis of diseases    resolved by renin-inhibition, comprising administration of the    compound or the pharmaceutically acceptable salt thereof, described    in any of (1), (a1) to (a10), (b1), (c1) to (c12) and (d1) to (d7).    (e9) a method for the treatment and/or prophylaxis of hypertension,    cardiac failure, diabetic nephropathy and the like, comprising    administration of the compound or the pharmaceutically acceptable    salt thereof, described in any of (1), (a1) to (a10), (b1), (c1) to    (c12) and (d1) to (d7).

The compound [I] of the present invention can be clinically used eitherin the free form or in the form of a pharmaceutically acceptable saltthereof. Examples of the pharmaceutically acceptable salt of thecompound [I] include a salt with an inorganic acid such ashydrochloride, sulfate, phosphate or hydrobromide, or a salt with anorganic acid such as acetate, fumarate, oxalate, citrate,methanesulfonate, benzenesulfonate, tosylate or maleate. Besides, whenthe compound [I] of the present invention has a carboxyl group(s) andthe like in its molecule, examples of the pharmaceutically acceptablesalt include, salts with a base such as alkaline metal (e.g., sodiumsalt, potassium salt) or alkaline earth metal (e.g., calcium salt).

The compound [I] of the present invention also includes a mixture of astereoisomer such as a geometrical isomer, a tautomer and an enantiomer,and an isolated stereoisomer thereof. In the compound [I] of the presentinvention, (R)-configuration is preferable for an asymmetric carbon atomof the morpholine ring having the substituent, T, from the view ofrenin-inhibition. From the view of renin-inhibition, (R)-configurationis also preferable for an asymmetric carbon atom which is substitutedwith R.,

The present invention also includes an intramolecular salt, a hydrate, apharmaceutically acceptable solvate and a crystal polymorph of thecompound [I]. Additionally it should be understood that the compound [I]of the present invention is not limited to the compounds described inthe examples below but includes whole the compounds of the formula [I]and pharmaceutically acceptable salts thereof.

Accordingly the compound of the present invention or thepharmaceutically acceptable salts thereof may be useful as an agent forprevention and/or treatment of hypertension, cardiac failure, diabeticnephropathy and the like, and can be advantageous as a medicine due toits low toxicity.

The compound [I] of the present invention or a pharmaceuticallyacceptable salt thereof can be either orally or parenterallyadministered, and can be formulated into a conventional pharmaceuticalpreparation such as tablets, granules, capsules, powders, injections orinhalants etc.

The dose of the compound [I] of the present invention or apharmaceutically acceptable salt thereof may vary in accordance with theadministration routes, and the ages, body weights and conditions of thepatients, but usually it is in the range of about 0.001 to 500 mg/kg,preferably in the range of about 0.1 to 100 mg/kg.

The compound [I] of the present invention can be prepared by thefollowing methods but should not be construed to be limited thereto.

Method for Preparing the Compound [I]

The compound [I] of the present invention or the pharmaceuticallyacceptable salt thereof can be prepared by deprotecting P¹ of thecompound of the formula [II];

wherein P¹ is a protecting group and the other symbols are the same asdefined above, and converting the product to a pharmaceuticallyacceptable salt thereof, if necessary.

Method for Preparing the Compound [II]

The compound [II] can be prepared by reacting a carboxylic compound ofthe formula [III]:

wherein the symbols are the same as defined above, or an activatedderivative thereof with an amine compound of the formula [IV];

(R²²RCH)R¹NH  [IV]

wherein the symbols are the same as defined above.

The compound of the present invention has two or more asymmetric carbonand the reaction product may be obtained as a mixture ofdiastereoisomers. Such a mixture of diastereoisomers can be separatedand purified by a usual method, a silica gel column chromatography forexample.

Reaction in the Method for Preparating the Compound [I]

Examples of the protecting group shown as P¹ or P² include a usualamino-protecting group such as a tert-butoxycarbonyl group, abenzyloxycarbonyl group, 4-methoxy benzyloxycarbonyl group, a benzylgroup, a 4-methoxybenzyl group, an acetyl group, a benzoyl group, atosyl group and the like.

The protecting group P¹ and P² of the compound [II] can be deprotectedby treating with acid or base or catalytic reduction or a deprotectingagent in a suitable solvent or without solvent. As an acid, an inorganicacid such as hydrochloric acid, sulfuric acid and the like, and anorganic acid such as acetic acid, trifluoroacetic acid, methanesulfonicacid, p-toluenesulfonic acid and the like can be preferably used. As abase, an inorganic base (e.g., an alkali metal hydride such as sodiumhydride, an alkali metal carbonate such as sodium carbonates andpotassium carbonates, an alkali metal amide such as sodium amides andlithium amide, an alkali metal alkoxide such as sodium methoxide, analkali metal such as sodium, and an alkali metal hydroxide such assodium hydroxide, potassium hydroxide etc.) and the like can bepreferably used. As a deprotecting agent, zinc bromide andtrimethylsilane trifluoromethanesulfonate etc. can be used. Thecatalytic reduction can be carried out by preferably using palladiumcarbon, palladium hydroxide carbon, platinum oxide and the like as acatalyst under hydrogen atmosphere. Examples of the solvent include anysolvent which does not disturb the reaction, such as methanol, ethanol,isopropyl alcohol, 1,4-dioxane, diethyl ether, tetrahydrofuran,methylene chloride, chloroform, dichloroethane, ethyl acetate, toluene,and a mixture thereof. The acid or the base described above can be usedas the solvent. The reaction can be suitably carried out at from −78° C.to a boiling temperature of the solvent.

Reaction in the Method for Preparating the Compound [II]

The compound [II] can be prepared by a condensation reaction of acarboxylic acid compound [III] and an amine compound [IV] in a suitablesolvent or without a solvent.

The condensation reaction can be carried out by a conventionalcondensation reaction in the presence of a condensing agent, or reactingan activated derivative of the compound [III] (e.g., an acid halide, amixed acid anhydride, an activated ester and the like) with the compound[IV], after the compound [III] is converted to the reactive derivativethereof. Examples of the condensing agent includeN,N-dicyclohexylcarbodiimide (DCC),1-ethyl-3-(3-dimethylaminopropyl)carbodiimide (EDC) or hydrochloridethereof, carbonyldiimidazole (CDI), diphenylphosphoryl azide (DPPA),diethyl cyanophosphonate (DEPC) and the like, and among them DCC, EDC orits hydrochloride is preferable.

When the reactive derivative of the compound [III] is used, the reactivederivative can be reacted with the compound [IV] in a suitable solventor without a solvent in presence of an acid scavenger if necessary,after the compound [III] is converted to an acid halide using ahalogenating agent (e.g., thionyl chloride, thionyl bromide, oxalylchloride and the like), a mixed acid anhydride using chlorocarbonateester (e.g., methyl chlorocarbonate, ethyl chlorocarbonate, isobutylchloroformate and the like) or acid chloride (2,4,6-trichlorobenzoylchloride and the like), or an activated ester of N-hydroxylaminecompound (1-hydroxysuccinimide, 1-hydroxybenzotriazole and the like) orof phenol compound (p-nitrophenol and the like) or a lower alcohol ester(methyl ester, ethyl ester and the like). In a method converting to anacid halide, an addition of catalyst such as dimethylformamide and thelike can accelerate the reaction. As an acid scavenger, an inorganicbase or an organic base is used when necessary, and examples of aninorganic base include sodium carbonate, potassium carbonate, cesiumcarbonate, sodium bicarbonate, sodium hydroxide, potassium hydroxide,lithium hydroxide and the like and examples of an organic base includetriethylamine, tributylamine, diisopropylethylamine,1,8-diazabicyclo[5,4,0]undeca-7-ene, N,N-diethylaniline, pyridine,lutidine, colidine and the like. In the present reaction, triethylamine,diisopropylethylamine, pyridine and the like are preferably used as anacid scavenger. When the acid scavenger is used in this reaction, acidscavenger is used as the solvent.

In the condensing reaction shown above, it can be conducted oraccelerated by adding 4-aminopyridine and the like

When using a solvent in the condensing reaction above, any inert solventwhich does not disturb the reaction can be used and examples of thesolvents include chloroform, dichloromethane, dichloroethane, toluene,diethyl ether, tetrahydrofuran, dioxane, 1,2-dimethoxyethane, ethylacetate, amide-related solvent (N,N-dimethylformamide,N,N-dimethylacetamide, 1,3-dimethyl-2-imidazolidinon etc.), pyridine,2,6-lutidine, water and the like, and a mixture thereof can be alsoused. Among them, chloroform, tetrahydrofuran, dioxane,N,N-dimethylformamide, N,N-dimethylacetamide, and a mixture ofchloroform and N,N-dimethylformamide etc. are preferred.

Usually the condensation reaction above can be carried out at atemperature from −20° C. to a reflux temperature of the solvent and ifnecessary, it can be carried out at a lower temperature which issuitably selected.

Examples of the compounds [I] of the present invention prepared by themethods illustrated above are shown below, but the present inventionshould not be construed to be limited thereto.

EXAMPLES Example 1(2R)—N-Cyclopropyl-N-{1-[4-(3-methoxypropoxy)-1-benzofuran-6-yl]ethyl}morpholine-2-carboxamide[Ex(1-1), Ex(1-2)]

To a solution of tert-butyl (2R)-2-[(cyclopropyl{1-[4-(3-methoxypropoxy)-1-benzofuran-6-yl]ethyl}amino)carbonyl]morpholine-4-carboxylate(200 mg) and 2,6-lutidine (0.142 mL) in chloroform (4 mL) was addedtrimethylsilyltriflate (0.180 mL) under ice-cooling, and the mixture wasstirred at the same temperature for 30 minutes. Then, thereto was addedaqueous saturated sodium hydrogen carbonate solution under ice-cooling,and the mixture was extracted with ethyl acetate. The organic layer waswashed with saturated saline, dried over magnesium sulfate, and thenconcentrated under reduced pressure. The resulting residue was purifiedby silica gel column chromatography (eluent: chloroform/methanol/ammoniawater=200/2/1) to give(2R)—N-cyclopropyl-N-{(1R)-1-[4-(3-methoxypropoxy)-1-benzofuran-6-yl]ethyl}morpholine-2-carboxamide[Ex(1-1)] (12.5 mg) and(2R)—N-cyclopropyl-N-{(1S)-1-[4-(3-methoxypropoxy)-1-benzofuran-6-yl]ethyl}morpholine-2-carboxamide[Ex(1-2)] (20.2 mg) as a colorless oil.

APCI-MS m/z: 403 [M+H]⁺.

Example 2(2R)—N-{(1R)-1-[3-Chloro-1-(3-methoxypropyl)-1H-indol-6-yl]ethyl}-N-cyclopropylpiperazine-2-carboxamide[Ex(2-1)]

To a solution of di-tert-butyl(2R)-2-{[{(1R)-1-[3-chloro-1-(3-methoxypropyl)-1H-indol-6-yl]ethyl}(cyclopropyl)amino]carbonyl}piperazine-1,4-dicarboxylate(44.0 mg) and 2,6-lutidine (0.050 mL) in dichloromethane (1.0 mL) wasadded trimethylsilyltriflate (0.051 mL) under ice-cooling, and themixture was stirred at the same temperature for 1 hour. Then, theretowere added aqueous saturated sodium hydrogen carbonate solution andmethanol (2.0 mL) under ice-cooling, and the mixture was extracted withchloroform. The organic layer was washed with saturated saline, driedover magnesium sulfate, and then concentrated under reduced pressure.The resulting residue was purified by NH-silica gel columnchromatography (eluent: ethyl acetate→ethyl acetate/methanol=5/1) togive(2R)—N-{(1R)-1-[3-chloro-1-(3-methoxypropyl)-1H-indol-6-yl]ethyl}-N-cyclopropylpiperazine-2-carboxamide[Ex(2-1)] (25.4 mg) as a colorless oil.

APCI-MS m/z: 419/421 [M+H]⁺.

Example 3(2R)—N—Cyclopropyl-N-{(1R)-1-[1-(3-methoxypropyl)-3-methyl-1H-indazol-6-yl]ethyl}-morpholine-2-carboxamidehydrochloride [Ex(3-1)]

To a solution of tert-butyl(2R)-2-[(cyclopropyl{(1R)-1-[1-(3-methoxypropyl)-3-methyl-1H-indazol-6-yl]ethyl}amino)carbonyl]morpholine-4-carboxylate(44.4 mg) in chloroform (2.0 mL) was added 4-normal hydrogenchloride-dioxane solution (0.75 mL) under ice-cooling, and the mixturewas stirred at room temperature for 20 hours. The reaction solution wasconcentrated under reduced pressure, and the resulting residue wasdissolved in water (1 mL), and then washed with diethyl ether. Theaqueous layer was freeze-dried to give(2R)—N-cyclopropyl-N-{(1R)-1-[1-(3-methoxypropyl)-3-methyl-1H-indazol-6-yl]ethyl}morpholine-2-carboxamidehydrochloride [Ex(3-1)] (25 mg) as a colorless powder.

APCI-MS m/z: 401 [M+H]⁺.

Example 4(2R)—N-{1-[4-Chloro-1-(3-methoxypropyl)-3-methyl-1H-indazol-6-yl]ethyl}-N-cyclopropylmorpholine-2-carboxamide [Ex(4-1), Ex(4-2)]

To a solution of tert-butyl(2R)-2-{[{1-[4-chloro-1-(3-methoxypropyl)-3-methyl-1H-indazol-6-yl]ethyl}(cyclopropyl)amino]carbonyl}morpholine-4-carboxylate(146 mg) in chloroform (2.0 mL) was added 4-normal hydrogenchloride-dioxane solution (2.0 mL) under ice-cooling, and the mixturewas stirred at room temperature for 3 hours. The reaction solution wasconcentrated under reduced pressure, and then thereto was added aqueoussaturated sodium hydrogen carbonate solution under ice-cooling, and themixture was extracted with chloroform. The organic layer was washed withsaturated saline, dried over magnesium sulfate, and then concentratedunder reduced pressure. The resulting residue was purified by silica gelcolumn chromatography (eluent: chloroform/methanol/ammoniawater=500/10/1) to give(2R)—N-{(1R)-1-[4-chloro-1-(3-methoxypropyl)-3-methyl-1H-indazol-6-yl]ethyl}-N-cyclopropylmorpholine-2-carboxamide[Ex(4-1)] (50.2 mg) and(2R)—N—{(1S)-1-[4-chloro-1-(3-methoxypropyl)-3-methyl-1H-indazol-6-yl]ethyl}-N-cyclopropylmorpholine-2-carboxamide[Ex(4-2)] (18.4 mg) as a colorless oil.

APCI-MS m/z: 435/437 [M+H]⁺.

Example 5A(2R)—N-{1-[3-Bromo-4-methoxy-5-(3-methoxypropoxy)phenyl]ethyl}-N-cyclopropylmorpholine-2-carboxamide[Ex(5-1), Ex(5-2)]

To a solution of tert-butyl(2R)-2-{[{1-[3-bromo-4-methoxy-5-(3-methoxypropoxy)-phenyl]ethyl}(cyclopropyl)amino]carbonyl}morpholine-4-carboxylate(135 mg) in dichloromethane (2 mL) was added trifluoroacetic acid (2mL), and the mixture was stirred at room temperature for 30 minutes. Thereaction solution was concentrated under reduced pressure, and to theresulting residue was added chloroform, and the mixture was filteredthrough (Bond-Elute: registered trademark) (NH₂). The filtrate wasconcentrated under reduced pressure, and the resulting residue waspurified by NH-silica gel column chromatography (eluent: ethylacetate/methanol/ammonia water=200/10/1) to give(2R)—N-{(1R)-1-[3-bromo-4-methoxy-5-(3-methoxypropoxy)phenyl]ethyl}-N-cyclopropylmorpholine-2-carboxamide[Ex(5-1)] (53.7 mg) and(2R)—N-{(1S)-1-[3-bromo-4-methoxy-5-(3-methoxypropoxy)phenyl]ethyl}-N-cyclopropylmorpholine-2-carboxamide[Ex(5-2)] (30.5 mg) as a colorless oil.

APCI-MS m/z: 471/473[M+H]⁺.

Example 5B

Methyl

{3-[3-((1-{cyclopropyl[(2R)-morpholin-2-ylcarbonyl]amino}ethyl)-1H-pyrrolo[2,3-b]-pyridin-1-yl]propyl}carbamate

To a solution of t-butyl(2R)-2-({cyclopropyl[1-(1-{3-[(methoxycarbonyl)amino]propyl-1H-pyrrolo[2,3-b]pyridin-3-yl)ethyl]amino}carbonyl)morpholin-4-carboxylate(116 mg) and 2,6-lutidine (0.077 mL) in dichloromethane (2 mL) was addedtrimethylsilyltriflate (0.099 mL) under ice-cooling, and the mixture wasstirred at the same temperature for 1 hour. Then, thereto were addedaqueous sodium hydrogen carbonate solution and methanol (2.0 mL) underice-cooling, and the mixture was extracted with chloroform. The organiclayer was washed with saturated saline, dried over magnesium sulfate,and then concentrated under reduced pressure. The resulting residue waspurified by NH-silica gel column chromatography (eluent: ethylacetate→ethyl acetate/methanol=9/1) to give methyl{3-[3-(1-cyclopropyl[(2R)-morpholin-2-ylcarbonyl]aminoethyl)-1H-pyrrolo[2,3-b]pyridin-1-yl]propyl}carbamate (50 mg) as acolorless oil. Methyl{3-[3-(1-cyclopropyl[(2R)-morpholin-2-ylcarbonyl]aminoethyl)-1H-pyrrolo[2,3-b]pyridin-1-yl]propyl}carbamate (40 mg) wasseparated by CHIRALPAK IC (eluent:n-hexane/ethanol/diethylamine=50/50/0.1; instrument: Waters 302 (600Esystem)) into diastereomers to give methyl{3-[3-((1R)-1-cyclopropyl[(2R)-morpholin-2-ylcarbonyl]amino-ethyl)-1H-pyrrolo[2,3-b]pyridin-1-yl]propyl}carbamate(18 mg) as a colorless oil and methyl{3-[3-((1S)-1-cyclopropyl[(2R)-morpholin-2-ylcarbonyl]amino}ethyl)-1H-pyrrolo[2,3-b]pyridin-1-yl]propyl}carbamate(18 mg) as a colorless oil.

APCI-MS m/z: 430 [M+H]⁺.

Examples 6 to 160

The following nitrogen-containing saturated heterocyclic compounds, etc.were prepared in the similar manner to the above Examples 1 to 5. Eachsymbol of Methods A to C refers to each method according to thefollowing method of Examples.

Method A: Examples 1, 2

Method B: Examples 3, 4

Method C: Example 5A

TABLE 1 EX. No. Chemical formula a MW b c d e 6

392.4928 B 393 [M + H]+ O 7

HCl 360.8826 B 325 [M + H]+ P 8

HCl 360.8826 B 325 [M + H]+ P 9

HCl 428.9538 B 393 [M + H]+ O

TABLE 2 10

HCl 436.9808 B 401 [M + H]+ P 11

HCl 398.928 B 363 [M + H]+ P 12

HCl 370.8744 B 335 [M + H]+ O 13

334.4134 A 335 [M + H]+ O

TABLE 3 14

334.4134 A 335 [M + H]+ O 15

348.4402 A 349 [M + H]+ O 16

348.4402 A 349 [M + H]+ O 17

HCl 436.9808 B 401 [M + H]+ P

TABLE 4 18

374.478 A 375 [M + H]+ O 19

376.4938 A 377 [M + H]+ O 20

380.4571 A 381 [M + H]+ O 21

392.4928 A 393 [M + H]+ O

TABLE 5 22

376.4938 A 377 [M + H]+ O 23

380.4571 A 381 [M + H]+ O 24

392.4928 A 393 [M + H]+ O 25

376.4938 A 377 [M + H]+ O

TABLE 6 26

376.4938 A 377 [M + H]+ O 27

374.478 A 375 [M + H]+ O 28

419.95 A 420/422 [M + H]+ O 29

419.95 A 420/422 [M + H]+ O

TABLE 7 30

418.9659 A 419/421 [M + H]+ O 31

418.9659 A 419/421 [M + H]+ O 32

380.4571 A 381 [M + H]+ O 33

380.4571 A 381 [M + H]+ O

TABLE 8 34

HCl 446.817 B 411 [M + H]+ P 35

453.38 A 453/455 [M + H]+ O 36

414.5466 A 415 O 37

418.5099 A 419 [M + H]+ O

TABLE 9 38

418.5099 A 419 [M + H]+ O 39

434.9649 B 435/437 [M + H]+ O 40

434.9649 B 435/437 [M + H]+ O 41

414.5466 B 415 [M + H]+ O

TABLE 10 42

429.5179 A 430 [M + H]+ O 43

402.488 A 403 [M + H]+ O 44

402.488 A 403 [M + H]+ O 45

450.5722 A 451 [M + H]+ O

TABLE 11 46

450.5722 A 451 [M + H]+ O 47

368.8585 A 369/371 [M + H]+ O 48

368.8585 A 369/371 [M + H]+ O 49

368.8585 A 369/371 [M + H]+ O

TABLE 12 50

387.4771 A 388 [M + H]+ P 51

387.4771 A 388 [M + H]+ P 52

355.4355 B 356 [M + H]+ P 53

355.4355 B 356 [M + H]+ O

TABLE 13 54

HCl 390.9084 B 355 [M + H]+ P 55

404.5038 A 405 [M + H]+ O 56

404.5038 A 405 [M + H]+ O 57

468.5169 A 469 [M + H]+ O

TABLE 14 58

468.5169 A 469 [M + H]+ O 59

385.5049 A 386 O 60

387.5207 A 388 [M + H]+ O 61

385.5049 A 386 O

TABLE 15 62

418.9659 A 419/421 [M + H]+ O 63

418.9659 A 419/421 [M + H]+ O 64

400.5198 A 401 [M + H]+ O 65

400.5198 A 401 [M + H]+ O

TABLE 16 66

HCl 477.9859 B 442 [M + H]+ P 67

HCl 477.9859 B 442 [M + H]+ P 68

HCl 420.9818 C 385 [M + H]+ O 69

414.5466 B 415 [M + H]+ O

TABLE 17 70

418.5099 A 419 [M + H]+ O 71

418.5099 A 419 [M + H]+ O 72

434.9649 A 435/437 [M + H]+ O 73

434.9649 A 435/437 [M + H]+ O

TABLE 18 74

388.5048 A 389 [M + H]+ O 75

399.5357 A 400 [M + H]+ O 76

401.5039 A 402 [M + H]+ O 77

388.5048 A 389 [M + H]+ O

TABLE 19 78

399.5317 A 400 [M + H]+ O 79

418.5099 A 419 [M + H]+ O 80

418.5099 A 419 [M + H]+ O 81

401.5039 A 402 [M + H]+ O

TABLE 20 82

398.5476 A 399 [M + H]+ O 83

398.5476 A 399 [M + H]+ O 84

387.4771 A 388 [M + H]+ O 85

410.4829 A 411 [M + H]+ O

TABLE 21 86

430.4641 A 431 [M + H]+ O 87

414.5466 A 415 [M + H]+ O 88

467.4009 A 467/469 [M + H]+ O 89

471.3889 C 471/473 [M + H]+ O

TABLE 22 90

471.3889 C 471/473 [M + H]+ O 91

433.9808 A 434/436 [M + H]+ O 92

433.9808 A 434/436 [M + H]+ O 93

413.5625 A 414 [M + H]+ O

TABLE 23 94

413.5625 A 414 [M + H]+ O 95

403.5197 A 404 [M + H]+ O 96

403.51971 A 404 [M + H]+ O 97

400.5198 C 401 [M + H]+ O

TABLE 24 98

400.5198 C 401 [M + H]+ O 99

386.493 C 387 [M + H]+ O 100

386.493 C 387 [M + H]+ O 101

417.5029 A 418 [M + H]+ O

TABLE 25 102

417.5029 A 418 [M + H]+ O 103

402.488 A 403 [M + H]+ O 104

402.488 A 403 [M + H]+ O 105

404.5038 A 405 [M + H]+ O

TABLE 26 106

404.5038 A 405 [M + H]+ O 107

416.5188 A 417 [M + H]+ O 108

416.5188 A 417 [M + H]+ O 109

416.5188 A 417 [M + H]+ O

TABLE 27 110

416.5188 A 417 [M + H]+ O 111

HCl 378.8578 B 343 [M + H]+ P 112

HCl 378.8578 B 343 [M + H]+ P 113

330.4254 A 331 [M + H]+ P

TABLE 28 114

400.5198 A 401 [M + H]+ O 115

402.5109 A 403 [M + H]+ O 116

398.5476 A 399 [M + H]+ O 117

405.4919 C 406 [M + H]+ O

TABLE 29 118

419.5187 C 420 [M + H]+ O 119

433.5455 C 434 [M + H]+ O 120

2*HCl 432.7768 B 360/362 [M + H]+ P 121

2*HCl 432.7768 B 360/362 [M + H]+ P

TABLE 30 122

386.489 A 387 [M + H]+ O 123

386.489 C 387 [M + H]+ O 124

386.493 A 387 [M + H]+ O 125

386.493 A 387 [M + H]+ O

TABLE 31 126

426.9379 C 427/429 [M + H]+ O 127

426.9379 C 427/429 [M + H]+ O 128

401.5079 C 402 [M + H]+ O 129

401.5079 C 402 [M + H]+ O

TABLE 32 130

384.5208 C 385 [M + H]+ O 131

384.5208 C 385 [M + H]+ O 132

468.5904 A 469 [M + H]+ O 133

495.6203 A 496 [M + H]+ O

TABLE 33 134

495.6203 A 496 [M + H]+ O 135

428.473 C 429 [M + H]+ O 136

386.493 A 387 [M + H]+ O 137

420.9381 A 421/423 [M + H]+ O

TABLE 34 138

420.9381 A 421/423 [M + H]+ O 139

390.5206 A 391 [M + H]+ O 140

400.5198 A 401 [M + H]+ O 141

404.4831 A 405 [M + H]+ O

TABLE 35 142

404.4831 A 405 [M + H]+ O 143

399.5357 A 400 [M + H]+ O 144

414.503 C 415 [M + H]+ O 145

414.503 C 415 [M + H]+ O

TABLE 36 146

386.493 A 387 [M + H]+ O 147

416.5188 C 417 [M + H]+ O 148

416.5188 C 417 [M + H]+ O 149

406.5196 A 407 [M + H]+ O

TABLE 37 150

420.5464 A 421 [M + H]+ O 151

450.5722 A 451 [M + H]+ O 152

414.5466 A 415 [M + H]+ O 153

414.5466 A 415 [M + H]+ O

TABLE 38 154

400.5145 A 401 [M + H]+ O 155

400.5145 A 401 [M + H]+ O 156

429.5126 C 430 [M + H]+ O 157

447.5031 C 448 [M + H]+ O

TABLE 39 158

447.5031 C 448 [M + H]+ O 159

400.5198 A 401 [M + H]+ O 160

400.5198 A 401 [M + H]+ O

Ex. No.: Example Number

a: Salt

b: Method

C: MS Results APCI

d: Ion species

e: Form

O: Oil

P: Powder

Example 161 tert-Butyl(2R)-2-[(cyclopropyl{1-[4-(3-methoxypropoxy)-1-benzofuran-6-yl]ethyl}amino)-carbonyl]morpholine-4-carboxylate[Ex(161-1)]

To a solution ofN-{1-[4-(3-methoxypropoxy)-1-benzofuran-6-yl]ethyl}-cyclopropanamine(200 mg) and (2R)-4-(tert-butoxycarbonyl)morpholine-2-carboxylic acid(240 mg) in N,N-dimethylformamide (7 mL) were added1-ethyl-3-(3-dimethylaminopropyl)carbodiimide hydrochloride (212 mg) and1-hydroxybenzotriazole (140 mg) under ice-cooling, and then stirred atroom temperature for 18 hours. To the reaction mixture was added aqueoussaturated sodium hydrogen carbonate solution, and the mixture wasextracted with ethyl acetate. The organic layer was sequentially washedwith water, 10% aqueous citric acid solution and saturated saline, andthen concentrated under reduced pressure to give tert-butyl(2R)-2-[(cyclopropyl{1-[4-(3-methoxypropoxy)-1-benzofuran-6-yl]ethyl}amino)carbonyl]morpholine-4-carboxylate[Ex(161-1)] (306 mg) as a yellow oil.

APCI-MS m/z: 503 [M+H]⁺.

Example 162 tert-Butyl(2R)-2-[(cyclopropyl{1-[1-(3-methoxypropyl)-3-methyl-1H-indazol-6-yl]ethyl}amino)-carbonyl]morpholine-4-carboxylate[Ex(162-1), Ex(162-2)]

To a solution ofN-{1-[1-(3-methoxypropyl)-3-methyl-1H-indazol-6-yl]ethyl}-cyclopropanamine(2.63 g) and (2R)-4-(tert-butoxycarbonyl)morpholine-2-carboxylic acid(2.32 g) in N,N-dimethylformamide (25 mL) were added1-hydroxybenzotriazole (1.36 g) and1-ethyl-3-(3-dimethylaminopropyl)carbodiimide hydrochloride (2.11 g)under ice-cooling, and then the mixture was stirred at room temperaturefor 3 hours. To the reaction mixture was added ethyl acetate, and themixture was washed with saturated saline, dried over magnesium sulfate,and then concentrated under reduced pressure. The resulting residue waspurified by silica gel column chromatography (eluent: n-hexane/ethylacetate/methanol=30/3/1→9/3/1) to give tert-butyl (2R)-2-[(cyclopropyl{(1R)-1-[1-(3-methoxypropyl)-3-methyl-1H-indazol-6-yl]ethyl}amino)carbonyl]morpholine-4-carboxylate[Ex(162-1)] (1.71 g) and tert-butyl (2R)-2-[(cyclopropyl{(1S)-1-[1-(3-methoxypropyl)-3-methyl-1H-indazol-6-yl]ethyl}amino)carbonyl]morpholine-4-carboxylate[Ex(162-2)] (468 mg) as a colorless oil.

APCI-MS m/z: 435 [M+H]⁺.

Example 163 tert-Butyl(2R)-2-({cyclopropyl[1-(2-naphthyl)ethyl]amino}carbonyl)morpholine-4-carboxylate[Ex(163-1)]

To a solution of N-[1-(2-naphthyl)ethyl]cyclopropylamine hydrochloride(149 mg), (2R)-4-(tert-butoxycarbonyl)morpholine-2-carboxylic acid (208mg) and 1-hydroxybenzotriazole (122 mg) in N,N-dimethylformamide (6 mL)was added diisopropylethylamine (0.125 μL), and the thereto was added1-ethyl-3-(3-dimethylaminopropyl)carbodiimide hydrochloride (207 mg)under ice-cooling. The mixture was stirred at room temperature for 19hours, and then to the reaction solution was added 1-normal aqueoussodium hydrogen carbonate solution, and the mixture was extracted withethyl acetate. The organic layer was sequentially washed with watertwice, saturated saline, dried over sodium sulfate, and thenconcentrated under reduced pressure. The residue was triturated withn-hexane-diethyl ether (5:1) to give tert-butyl(2R)-2-(cyclopropyl[1-(2-naphthyl)ethyl]aminocarbonyl)morpholine-4-carboxylate [Ex(163-1)] (153 mg) as a colorlesspowder.

APCI-MS m/z: 425 [M+H]⁺.

Example 164 Di-tert-butyl(2R)-2-{[{1-[3-chloro-1-(3-methoxypropyl)-1H-indol-6-yl]ethyl}(cyclopropyl)-amino]carbonyl}piperazine-1,4-dicarboxylate[Ex(164-1), Ex(164-2)]

To a solution ofN-{1-[3-chloro-1-(3-methoxypropyl)-1H-indol-6-yl]ethyl}-cyclopropanamine(60 mg) and piperazinecarboxylic acid (77.5 mg) in dichloromethane (2mL) were added diisopropylethylamine (0.085 mL) and diphenylphosphorochloridate (0.037 mL) under ice-cooling, and the mixture wasstirred at room temperature for 20 hours. To the reaction solution wasadded 0.5% aqueous hydrochloric acid solution under ice-cooling, and themixture was extracted with ethyl acetate. The organic layer was washedwith saturated saline, dried over magnesium sulfate, and thenconcentrated under reduced pressure. The resulting residue was purifiedby silica gel column chromatography (eluent: n-hexane/ethylacetate=99/1→2/1) to give di-tert-butyl(2R)-2-{[{(1R)-1-[3-chloro-1-(3-methoxypropyl)-1H-indol-6-yl]ethyl}(cyclopropyl)amino]carbonyl}piperazine-1,4-dicarboxylate[Ex(164-1)] (47.5 mg) and di-tert-butyl(2R)-2-{[{(1S)-1-[3-chloro-1-(3-methoxypropyl)-1H-indol-6-yl]ethyl}-(cyclopropyl)amino]carbonyl}piperazine-1,4-dicarboxylate[Ex(164-2)] (26.8 mg) as a colorless oil.

APCI-MS m/z: 636/638 [M+H]⁺.

Example 165 Di-tert-butyl(2R)-2-[(cyclopropyl{1-[1-(3-methoxypropyl)-3,4-dimethyl-1H-indazol-6-yl]-ethyl}amino]carbonyl}piperazine-1,4-dicarboxylate[Ex(165-1), EX(165-2)]

To a solution ofN-{1-[1-(3-methoxypropyl)-3,4-dimethyl-1H-indazol-6-yl]ethyl}cyclopropanamine(137 mg) and piperazinecarboxylic acid (180 mg) in dichloromethane (2.5mL) were added diisopropylethylamine (0.20 mL) andbis(2-oxo-3-oxazolidinyl)phosphinic chloride (150 mg) under ice-cooling,and the mixture was stirred at room temperature for 15 hours. To thereaction solution was added water under ice-cooling, and the mixture wasextracted with ethyl acetate. The organic layer was washed withsaturated saline, dried over magnesium sulfate, and then concentratedunder reduced pressure. The resulting residue was purified by silica gelcolumn chromatography (eluent: n-hexane/ethyl acetate=2/1→4/1) to givedi-tert-butyl(2R)-2-[(cyclopropyl{(1R)-1-[1-(3-methoxypropyl)-3,4-dimethyl-1H-indazol-6-yl]ethyl}amino]carbonylpiperazine-1,4-dicarboxylate [Ex(165-1)] (45.6 mg) and di-tert-butyl(2R)-2-[(cyclopropyl{(1S)-1-[1-(3-methoxypropyl)-3,4-dimethyl-1H-indazol-6-yl]ethyl}-amino]carbonyl}piperazine-1,4-dicarboxylate[Ex(165-2)] (53.8 mg) as a colorless oil.

APCI-MS m/z: 614 [M+H]⁺.

Example 166 tert-Butyl tert-butyl 2-[(cyclopropyl{1-[7-(3-methoxypropoxy)-2,3-dihydro-1-benzofuran-5-yl]-ethyl}amino)carbonyl]morpholine-4-carboxylate[Ex(166-1)]

To a solution of tert-butyl (2R)-2-[(cyclopropyl{1-[7-(3-methoxypropoxy)-1-benzofuran-5-yl]ethyl}amino)carbonyl]morpholine-4-carboxylate(200 mg) in ethanol (5.0 mL) was added 20% palladium hydroxide on carbon(100 mg), and the mixture was stirred under hydrogen for 5 hours. Aninsoluble was filtered, and then concentration under reduced pressuregave tert-butyl tert-butyl 2-[(cyclopropyl{1-[7-(3-methoxypropoxy)-2,3-dihydro-1-benzofuran-5-yl]ethyl}amino)carbonyl]morpholine-4-carboxylate[Ex(166-1)] (193 mg) as a colorless oil.

APCI-MS m/z: 505 [M+H]⁺.

Example 167 tert-Butyl(2R)-2-[[(1R)-5-bromo-6-(3-methoxypropoxy)-2,3-dihydro-1H-inden-1-yl]-(cyclopropyl)amino)carbonyl]morpholine-4-carboxylate[Ex(167-1)]

To a suspension of N-bromosuccinimide (23.4 mg) in dichloromethane (0.5mL) was added dropwise a solution of tert-butyl(2R)-2-({cyclopropyl[(1R)-6-(3-methoxypropoxy)-2,3-dihydro-1H-inden-1-yl]amino)carbonyl]morpholine-4-carboxylate (62.4 mg) in dichloromethane (1.5 mL) underice-cooling, and the mixture was stirred for 5 hours. The reactionsolution was concentrated under reduced pressure, and the resultingresidue was dissolved in ethyl acetate, and then sequentially washedwith 1-normal aqueous sodium hydrogen carbonate solution, saturatedsaline, dried over sodium sulfate, and then concentrated under reducedpressure to give a crude product of tert-butyl(2R)-2-{[[(1R)-5-bromo-6-(3-methoxypropoxy)-2,3-dihydro-1H-inden-1-yl](cyclopropyl)amino)carbonyl]morpholine-4-carboxylate[Ex(167-1)] (74 mg) as a yellow oil.

APCI-MS m/z: 553/555 [M+H]⁺.

Example 168 tert-Butyl(2R)-2-[(cyclopropyl{(1R)-1-[4-(3-methoxypropyl)quinazolin-2-yl]ethyl}amino)-carbonyl]morpholin-4-carboxylate[Ex(168-1)]

To a solution of tert-butyl(2R)-2-({cyclopropyl[(1R)-1-(4-oxo-3,4-dihydroquinazolin-2-yl)ethyl]amino}carbonyl)morpholin-4-carboxylate(88 mg), 3-methoxy-1-propanol (36 mg) and triphenylphosphine (105 mg) intetrahydrofuran (4 mL) was added dropwise diisopropyl azodicarboxylate(84 μL) under ice-cooling, and the mixture was stirred at roomtemperature for 19 hours. The reaction solution was concentrated, andthe resulting residue was purified by silica gel column chromatography(eluent: n-hexane/ethyl acetate=2/1→1/2) to give tert-butyl(2R)-2-[(cyclopropyl{(1R)-1-[4-(3-methoxypropyl)quinazolin-2-yl]ethyl}amino)carbonyl]morpholin-4-carboxylate[Ex(168-1)] (38 mg) as a colorless oil.

APCI-MS m/z: 515 [M+H]⁺.

Example 169 tert-Butyl(2R)-2-[(cyclopropyl{(1R)-1-[4-ethoxy-3-(3-methoxypropoxy)phenyl]ethyl}amino)-carbonyl]morpholin-4-carboxylate[Ex(169-2)]

(1)

To a solution of tert-butyl(2R)-2-{[{1-[4-(benzyloxy)-3-(3-methoxypropoxy)phenyl]-ethyl}(cyclopropyl)amino]carbonyl}morpholin-4-carboxylate(1.45 g) in methanol (12 mL) was added 10% palladium on carbon (200 mg),and the mixture was stirred under hydrogen for 2 hours. An insoluble wasfiltered, and then concentrated under reduced pressure. The resultingresidue was purified by silica gel column chromatography (eluent:n-hexane/ethyl acetate=2/1→1/2) to give tert-butyl(2R)-2-[(cyclopropyl{(1R)-1-[4-hydroxy-3-(3-methoxypropoxy)phenyl]ethyl}amino)carbonyl]morpholin-4-carboxylate[Ex(169-1)] (913 mg) as a colorless oil.

(2) Titled Compound [Ex(169-2)]

To a solution of the compound obtained in the above (1) (100 mg) inacetonitrile (1.0 mL) were added potassium carbonate (34.7 mg) andiodoethane (35.8 mg), and the mixture was heated to reflux for 20 hours.The reaction solution was cooled to room temperature, and then dilutedwith ethyl acetate. An insoluble was filtered, and then concentratedunder reduced pressure. The resulting residue was purified by NH-silicagel column chromatography (eluent: n-hexane/ethyl acetate=10/1→2/1) togive tert-butyl(2R)-2-[(cyclopropyl{(1R)-1-[4-ethoxy-3-(3-methoxypropoxy)phenyl]ethyl}amino)carbonyl]morpholin-4-carboxylate[Ex(169-2)] (60 mg) as a colorless oil.

APCI-MS m/z: 507 [M+H]⁺.

Example 170 Methyl{3-[3-(1-{cyclopropyl[(2R)-morpholin-2-ylcarbonyl]amino}ethyl)-1H-indazol-1-yl]-propyl}carbamate[Ex(170-2)]

(1) To a solution of methyl3-{1-[{[(2R)-4-(tert-butoxycarbonyl)morpholin-2-yl]carbonyl}-(cyclopropyl)amino]ethyl}-1H-indazole-1-carboxylate(695 mg) in methanol (10 mL) was added potassium carbonate (407 mg), andthe mixture was stirred at room temperature for 30 minutes. The reactionsolution was concentrated, and then thereto was added ethyl acetate, andthe mixture was washed with aqueous saturated sodium chloride solution.The organic layer was dried over magnesium sulfate, and thenconcentrated under reduced pressure to give tert-butyl(2R)-2-({cyclopropyl[1-(1-{3-[(methoxycarbonyl)amino]propyl}-1H-indazol-3-yl)-ethyl]amino}carbonyl)morpholine-4-carboxylate[Ex(170-1)] (577 mg) as a colorless powder.

APCI-MS m/z: 415 [M+H]⁺.

(2) To a solution of the compound obtained in (1) (90 mg) and methyl(3-bromopropyl)carbamate (64 mg) in N,N-dimethylformamide (2 mL) wasadded potassium carbonate (60 mg), and the mixture was stirred at roomtemperature for 18 hours. To the reaction solution was added ethylacetate, and the mixture was washed with aqueous saturated sodiumchloride solution, dried over magnesium sulfate, and then concentratedunder reduced pressure. The resulting residue was purified by silica gelcolumn chromatography (eluent: n-hexane/ethyl acetate=1/20→1/1) to givemethyl{3-[3-(1-{cyclopropyl[(2R)-morpholin-2-ylcarbonyl]amino}ethyl)-1H-indazol-1-yl]propyl}carbamate[Ex(170-2)] (74 mg) as a yellow oil.

Examples 171 to 267

The following N-protected nitrogen-containing saturated heterocycliccompounds were prepared in the similar manner to the above Examples 161to 169. Each symbol of Methods A to C refers to each method according tothe following method of Examples.

Method A: Examples 161 to 163

Method B: Example 164

Method C: Example 165

Method D: Example 166

Method E: Example 167

Method F: Example 168

Method G: Example 169

TABLE 40 EX. No. Structure a b c d e 171

492.609 O 493 [M + H]+ A 172

424.5378 P 425 [M + H]+ A 173

500.636 O 501 [M + H]+ A 174

434.5296 O 435 [M + H]+ A

TABLE 41 175

448.5564 O 449 [M + H]+ A 176

434.5296 O 435 [M + H]+ A 177

500.636 O 501 [M + H]+ A 178

599.7681 O 600 [M + H]+ B

TABLE 42 179

474.5942 O 492 [M + NH4]+ 180

476.61 O 477 [M + H]+ 181

476.61 O 494 [M + NH4]+ 182

480.5733 O 498 [M + NH4]+

TABLE 43 183

492.609 O 510 [M + NH4]+ A 184

480.5733 O 481 [M + H]+ A 185

535.0811 O 535/537 [M + H]+ A 186

514.6628 O 515 [M + H]+ A

TABLE 44 187

518.6261 O 519 [M + H]+ A 188

514.6628 O 515 [M + H]+ A 189

502.6042 O 503 [M + H]+ A 190

504.62 O 505 [M + H]+ D

TABLE 45 191

550.6884 O 568 [M + NH4]+ A 192

468.9747 O 469/471 [M + H]+ A 193

468.9747 P 469/471 [M + H]+ A 194

487.5933 O 488 [M + H]+ A

TABLE 46 195

510.4722 O 511 [M + H]+ A 196

553.4903 O 553/555 [M + H]+ E 197

529.6341 O 530 [M + H]+ A 198

454.5636 O 455 [M + H]+ A

TABLE 47 199

541.6411 P 542 [M + H]+ A 200

455.5517 P 456 [M + H]+ A 201

462.5832 O 463 [M + H]+ A 202

487.5933 O 505 [M + NH4]+ A

TABLE 48 203

530.5803 O 531 [M + H]+ A 204

484.637 O 485 [M + H]+ A 205

535.0811 O 535/537 [M + H]+ A 206

500.636 O 501 [M + H]+ A

TABLE 49 207

518.6261 O 519 [M + H]+ A 208

488.621 O 489 [M + H]+ A 209

567.5171 O 567/569 [M + H]+ E 210

510.5991 O 511 [M + H]+ A

TABLE 50 211

571.5051 O 571/573 [M + H]+ A 212

499.6479 O 500 [M + H]+ A 213

598.78 O 616 [M + NH4]+ B 214

634.2132 O 634/636 [M + H]+ C

TABLE 51 215

613.7949 O 614 [M + H]+ C 216

601.7363 O 602 [M + H]+ B 217

603.7521 O 604 [M + H]+ D 218

517.6191 O 518 [M + H]+ A

TABLE 52 219

486.6092 O 487 [M + H]+ A 220

500.636 O 501 [M + H]+ A 221

527.0541 O 527/529 [M + H]+ A 222

514.6628 O 515 [M + H]+ A

TABLE 53 223

430.5416 O 431 [M + H]+ A 224

502.6271 O 503 [M + H]+ A 225

498.6638 O 499 [M + H]+ A 226

533.6617 O 534 [M + H]+ A

TABLE 54 227

505.6081 O 506 [M + H]+ A 228

519.6349 O 520 [M + H]+ A 229

502.6042 O 503 [M + H]+ A 230

504.62 O 505 [M + H]+ D

TABLE 55 231

484.637 O 485 [M + H]+ A 232

516.635 O 517 [M + H]+ A 233

442.513 P 443 [M + H]+ A 234

442.513 P 443 [M + H]+ A

TABLE 56 235

459.971 P 460/462 [M + H]+ A 236

459.971 P 460/462 [M + H]+ A 237

514.6192 O 515 [M + H]+ F 238

514.6192 P 515 [M + H]+ F

TABLE 57 239

490.6368 O 491 [M + H]+ A 240

520.0662 O 520/522 [M + H]+ A 241

619.1983 O 636/638 [M + H]+ B 242

568.6331 O 569 [M + H]+ A

TABLE 58 243

487.6369 O 488 [M + H]+ A 244

487.6369 O 488 [M + H]+ A 245

485.6211 O 486 [M + H]+ A 246

486.6052 O 504 [M + NH4]+ A

TABLE 59 247

486.6092 O 487 [M + H]+ A 248

516.635 O 517 [M + H]+ A 249

516.635 O 517 [M + H]+ A 250

488.625 O 489 [M + H]+ A

TABLE 60 251

595.7365 O 596 [M + H]+ A 252

504.5993 O 505 [M + H]+ A 253

550.6884 O 568 [M + NH4]+ G 254

520.6626 O 521 [M + H]+ G

TABLE 61 255

506.6358 O 507 [M + H]+ G 256

568.7066 O 569 [M + H]+ A 257

528.5892 O 529 [M + H]+ A 258

500.636 O 501 [M + H]+ A

TABLE 62 259

486.6092 O 487 [M + H]+ A 260

521.0543 O 521/523 [M + H]+ A 261

518.6261 O 519 [M + H]+ A 262

486.6092 O 487 [M + H]+ A

TABLE 63 263

514.6628 O 515 [M + H]+ A 264

500.636 O 501 [M + H]+ A 265

529.6285 O 530 [M + H]+ H 266

547.6189 O [M + H]+ A

TABLE 64 267

472 5341 P 490 [M + NH4]+ A

Ex. No.: Example Number

a: Molecular weight

b: Properties

c: MS Results APCI

d: Ion species

e: Method

O: Oil

P: Powder

Examples 268 to 287

The following nitrogen-containing saturated heterocyclic compounds, etc.were prepared in the similar manner to the above Examples 1 to 5. Eachsymbol of Methods A to C refers to each method according to thefollowing method of Examples.

Method A: Examples 1, 2

Method B: Examples 3, 4

Method C: Example 5A

TABLE 65 EX. No. Structure a MW b c d e 268

430.5007 C 431 [M + H]⁺ O 269

430.5007 C 431 [M + H]⁺ O 270

444.5273 C 445 [M + H]⁺ O 271

444.5273 C 445 [M + H]⁺ O 272

414.5411 C 415 [M + H]⁺ O 273

414.5411 C 415 [M + H]⁺ O

Ex. No.: Example Number d: Ion species

a: Salt e: Form

b: Method O: Oil

c: MS Results APCI

TABLE 66 EX. No. Structure a MW b c d e 274

414.5411 A 415 [M + H]⁺ O 275

414.5411 A 415 [M + H]⁺ O 276

390.5164 A 391 [M + H]⁺ O 277

390.5164 A 391 [M + H]⁺ O 278

419.5145 A 420 [M + H]⁺ O 279

419.5145 A 420 [M + H]⁺ O

Ex. No.: Example Number d: Ion species

a: Salt e: Form

b: Method O: Oil

c: MS Results APCI

TABLE 67 EX. No. Structure a MW b c d e 280

420.5026 C 421 [M + H]⁺ O 281

420.5026 C 421 [M + H]⁺ O 282

418.5298 C 419 [M + H]⁺ O 283

418.5298 C 419 [M + H]⁺ O 284

420.5026 C 421 [M + H]⁺ O 285

420.5026 C 421 [M + H]⁺ O

Ex. No.: Example Number d: Ion species

a: Salt e: Form

b: Method O: Oil

c: MS Results APCI

TABLE 68 EX. No. Structure a MW b c d e 286

418.5298 C 419 [M + H]⁺ O 287

418.5298 C 419 [M + H]⁺ O

Ex. No.: Example Number

a: Salt

b: Method

c: MS Results APCI

d: Ion species

e: Form

O: Oil

Examples 288 to 294

The following N-protected nitrogen-containing saturated heterocycliccompounds were prepared in the similar manner to the above Examples 161to 163 (Method A).

TABLE 69 EX. No. Structure a b c d e 288

530.6165 O 531 [M + H]⁺ A 289

544.6431 O 545 [M + H]⁺ A 290

514.6569 O 515 [M + H]⁺ A 291

514.6569 O 515 [M + H]⁺ A 292

514.6569 O 515 [M + H]⁺ A 293

490.6322 O 491 [M + H]⁺ A

Ex. No.: Example Number

a: Molecular weight

b: Properties

C: MS Results APCI

d: Ion species

e: Method

O: Oil

TABLE 70 EX. No. Structure a b c d e 294

518.6456 O 519 [M + H]⁺ A

Ex. No.: Example Number

a: Molecular weight

b: Properties

C: MS Results APCI

d: Ion species

e: Method

O: Oil

Example 295

1) To a solution of 1-(3-iodo-4-methoxyphenyl)ethanone (10 g) indiethylamine (181 mL) were added benzyl prop-2-yn-1-ylcarbamate (8.2 g),dichlorobis(triphenylphosphine)palladium (II) (2.54 g) and copper (I)iodide (689 mg), and the mixture was stirred at 50° C. for 2 hours. Thereaction solution was cooled, and then thereto was added water, and themixture was extracted with chloroform. The organic layer wassequentially washed with 2-normal hydrochloric acid, water, and thendried over magnesium sulfate, and concentrated under reduced pressure.The resulting residue was purified by silica gel column chromatography(eluent: chloroform→chloroform/ethyl acetate=1/1) to givebenzyl[3-(5-acetyl-2-methoxyphenyl)prop-2-yn-1-yl]carbamate [Ex(295-1)](5.3 g) as a red solid.

APCI-MS m/z: 355[M+NH₄]⁺.

2) Benzyl[3-(5-acetyl-2-methoxyphenyl)prop-2-yn-1-yl]carbamate andcyclopropylamine were treated in the similar manner to Reference Example6(6) to give benzyl(3-{5-[1-(cyclopropylamino)ethyl]-2-methoxyphenyl}prop-2-yn-1-yl)carbamate[Ex(295-2)] as a yellow oil.

APCI-MS m/z: 379 [M+H]⁺.

3) Benzyl(3-{5-[1-(cyclopropylamino)ethyl]-2-methoxyphenyl}prop-2-yn-1-yl)carbamateand (2R)-4-(tert-butoxycarbonyl)morpholine-2-carboxylic acid weretreated in the similar manner to Example 162 to give tert-butyl(2R)-2-{[{1-[3-(3-[(benzyloxy)carbonyl]aminoprop-1-yn-1-yl)-4-methoxyphenyl]ethyl}(cyclopropyl)amino]carbonyl}morpholine-4-carboxylate[Ex(295-3)] as a colorless solid.

APCI-MS m/z: 609[M+NH₄]⁺.

4) To a solution of tert-butyl(2R)-2-{[{1-[3-(3-[(benzyloxy)carbonyl]amino}prop-1-yn-1-yl)-4-methoxyphenyl]ethyl}(cyclopropyl)amino]carbonyl}morpholin-4-carboxylate(2.82 g) in methanol (25 mL) was added 10% palladium on carbon (282 mg),and the mixture was stirred under hydrogen for 7 hours. An insoluble wasfiltered off, and then the filtrate was concentrated under reducedpressure, and the resulting residue was purified by NH-silica gel columnchromatography (eluent: chloroform→chloroform/methanol=33/1) to givetert-butyl (2R)-2-[1-[3-(3-aminopropyl)-4-methoxyphenyl]ethyl(cyclopropyl)amino]carbonyl morpholin-4-carboxylate [Ex(295-4)] (1.49 g)as a colorless oil.

APCI-MS m/z: 462 [M+H]⁺.

5) tert-Butyl(2R)-2-{[{1-[3-(3-aminopropyl)-4-methoxyphenyl]ethyl}(cyclopropyl)amino]-carbonyl}morpholin-4-carboxylaterand methyl chloroformate were treated in the similar manner to ReferenceExample 28(5) to give tert-butyl(2R)-2-({cyclopropyl[1-(4-methoxy-3-{3-[(methoxycarbonyl)amino]propyl}phenyl)ethyl]amino}carbonyl)morpholin-4-carboxylate[Ex(295-5)] as a colorless oil.

APCI-MS m/z: 504 [M+H]⁺.

Example 296

1) 5-Bromo-6-methoxynicotinic acid and N,O-dimethylhydroxylaminehydrochloride were treated in the similar manner to Reference Example7(5), and then the resulting compound and methylmagnesium bromide weretreated in the similar manner to Reference Example 7(6) to give1-(5-bromo-6-methoxypyridin-3-yl)ethanone [Ex(296-1)] as a colorlesspowder.

APCI-MS m/z: 230/232 [M+H]⁺.

2) Bis(benzonitrile)dichloropalladium (II) (12 mg) and copper (I) iodide(3.8 mg) were added to 1,4-dioxane (1 mL) under argon, and then theretowere added a solution of 10% tri-t-butylphosphine in hexane (179 μL),diisopropylamine (168 μL), 1-(5-bromo-6-methoxypyridin-3-yl)ethanone(230 mg) and a solution of methyl prop-2-yn-1-ylcarbamate (149 mg) in1,4-dioxane (1 mL). The mixture was stirred at room temperature for 5hours. The reaction solution was concentrated under reduced pressure,and the resulting residue was purified by silica gel columnchromatography (eluent: n-hexane/ethyl acetate=3/1→1/3) to give methyl[3-(5-acetyl-2-methoxypyridin-3-yl)prop-2-yn-1-yl]carbamate [Ex(296-2)](188 mg) as a pale yellow powder.

APCI-MS m/z: 263 [M+H]⁺.

3) Methyl[3-(5-acetyl-2-methoxypyridin-3-yl)prop-2-yn-1-yl]carbamate andcyclopropylamine were treated in the similar manner to Reference Example6(6) to give methyl(3-{5-[1-(cyclopropylamino)ethyl]-2-methoxypyridin-3-yl}prop-2-yn-1-yl)carbamate[Ex(296-3)] as a colorless oil.

APCI-MS m/z: 304 [M+H]⁺.

4) Methyl(3-{5-[1-(cyclopropylamino)ethyl]-2-methoxypyridin-3-yl}prop-2-yn-1-yl)carbamateand (2R)-4-(tert-butoxycarbonyl)morpholine-2-carboxylic acid weretreated in the similar manner to Example 162 to give tert-butyl(2R)-2-({cyclopropyl[1-(6-methoxy-5-{3-[(methoxycarbonyl)amino]prop-1-yn-1-yl}pyridin-3-yl)ethyl]amino}carbonyl)morpholine-4-carboxylate[Ex(296-4)] as a colorless oil.

APCI-MS m/z: 517 [M+H]⁺.

5) To a solution of tert-butyl(2R)-2-({cyclopropyl[1-(6-methoxy-5-{3-[(methoxycarbonyl)-amino]prop-1-yn-1-yl}pyridin-3-yl)ethyl]aminocarbonyl)morpholin-4-carboxylate (150 mg) in ethyl acetate (2.5mL)-tetrahydrofuran (2.5 mL) was added 10% palladium on carbon (30 mg),and the mixture was stirred under hydrogen for 45 minutes. An insolublewas filtered off, and then the filtrate was concentrated under reducedpressure, and the resulting residue was purified by NH-silica gel columnchromatography (eluent: ethyl acetate) to give tert-butyl(2R)-2-({cyclopropyl[1-(6-methoxy-5-{3-[(methoxycarbonyl)amino]propyl}pyridin-3-yl)ethyl]amino}carbonyl)morpholine-4-carboxylate[Ex(296-5)] (159 mg) as a colorless oil.

APCI-MS m/z: 521 [M+H]⁺.

Example 297

1) 1-(2,6-Dichloropyridin-4-yl)ethanone and cyclopropylamine weretreated in the similar manner to Reference Example 6(6) to giveN-[1-(2,6-dichloropyridin-4-yl)ethyl]-cyclopropylamine [Ex(297-1)] as apale yellow oil.

APCI-MS m/z: 231/233 [M+H]⁺.

2) To a solution ofN-[1-(2,6-dichloropyridin-4-yl)ethyl]cyclopropylamine (430 mg) intetrahydrofuran (5 mL) were added di-tert-butyl dicarbonate (487 mg) andtriethylamine (518 μL), and the mixture was heated to reflux for 24hours. The reaction solution was cooled, and then thereto was addedwater, and the mixture was extracted with ethyl acetate. The organiclayer was washed with saturated saline, dried over sodium sulfate, andthen concentrated under reduced pressure. The resulting residue waspurified by silica gel column chromatography (eluent: n-hexane/ethylacetate=10/1→4/1) to give tert-butylcyclopropyl[1-(2,6-dichloropyridin-4-yl)ethyl]carbamate [Ex(297-2)] (420mg) as a pale yellow oil.

APCI-MS m/z: 331/333 [M+H]⁺.

3) To a solution of tert-butylcyclopropyl[1-(2,6-dichloropyridin-4-yl)ethyl]carbamate (155 mg) indimethoxyethane (4 mL) were added methyl[(2E)-3-(4,4,5,5-tetramethyl-1,3,2-dioxaboran-2-yl)prop-2-en-1-yl]carbamate(113 mg), 2M potassium carbonate (257 μL) andtetrakis(triphenylphosphine)palladium (0) (27 mg), and the mixture washeated to reflux for 3 hours. The reaction solution was cooled, and thenthereto was added water, and the mixture was extracted with ethylacetate. The organic layer was washed with saturated saline, dried overmagnesium sulfate, and then concentrated under reduced pressure. Theresulting residue was purified by silica gel column chromatography(eluent: n-hexane/ethyl acetate=3/1→ethyl acetate) to give methyl[(2E)-3-(4-{1-[(tert-butoxycarbonyl)(cyclopropyl)amino]ethyl}-6-chloropyridin-2-yl)prop-2-en-1-yl]carbamate[Ex(297-3)] (98 mg) as a pale yellow oil.

APCI-MS m/z: 410/412 [M+H]⁺.

4) Methyl[(2E)-3-(4-{1-[(tert-butoxycarbonyl)(cyclopropyl)amino]ethyl-6-chloropyridin-2-yl)-prop-2-en-1-yl]carbamateand vinyl boronic acid pinacol ester were treated in the similar mannerto Reference Example 113(6) to give methyl[(E)-2-(4-{1-[(tert-butoxycarbonyl)(cyclopropyl)amino]ethyl}-6-vinylpyridin-2-yl)vinyl]carbamate[Ex(297-4)] as a yellow oil.

APCI-MS m/z: 402 [M+H]⁺.

5) Methyl[(E)-2-(4-{1-[(tert-butoxycarbonyl)(cyclopropyl)amino]ethyl}-6-vinylpyridin-2-yl)vinyl]carbamateand trifluoroacetic acid were treated in the similar manner to ReferenceExample 113(8) to give methyl((E)-2-{4-[1-(cyclopropylamino)ethyl]-6-vinylpyridin-2-yl}vinyl)carbamate[Ex(297-5)] as a pale yellow oil.

APCI-MS m/z: 302 [M+H]⁺.

6) Methyl((E)-2-{4-[1-(cyclopropylamino)ethyl]-6-vinylpyridin-2-yl}vinyl)carbamateand (2R)-4-(tert-butoxycarbonyl)morpholin-2-carboxylic acid were treatedin the similar manner to Example 162 to give tert-butyl(2R)-2-({cyclopropyl[1-(2-{(1E)-3-[(methoxycarbonyl)amino]prop-1-en-1-yl}-6-vinylpyridin-4-yl)ethyl]amino}carbonyl)morpholin-4-carboxylate[Ex(297-6)] as a yellow oil. APCI-MS m/z: 515 [M+H]⁺.7) tert-Butyl(2R)-2-({cyclopropyl[1-(2-{(1E)-3-[(methoxycarbonyl)amino]prop-1-en-1-yl}-6-vinylpyridin-4-yl)ethyl]amino}carbonyl)morpholine-4-carboxylatewas reduced in the similar manner to Example 296(5) to give tert-butyl(2R)-2-({cyclopropyl[1-(2-ethyl-6-{3-[(methoxycarbonyl)amino]propyl}pyridin-4-yl)ethyl]amino}carbonyl)morpholin-4-carboxylate[Ex(297-7)] as a colorless oil.

APCI-MS m/z: 519 [M+H]⁺.

Example 298

1) 1-(2-Chloro-6-methoxypyridin-4-yl)ethanone and methyl[(2E)-3-(4,4,5,5-tetramethyl-1,3,2-dioxaboran-2-yl)prop-2-en-1-yl]carbamatewere treated in the similar manner to Example 297(3) to give methyl[(2E)-3-(4-acetyl-6-methoxypyridin-2-yl)prop-2-en-1-yl]carbamate[Ex(298-1)] as a colorless powder.

APCI-MS m/z: 265 [M+H]⁺.

2) Methyl[(2E)-3-(4-acetyl-6-methoxypyridin-2-yl)prop-2-en-1-yl]carbamate andcyclopropylamine were treated in the similar manner to Reference Example6(6) to give methyl((2E)-3-{4-[1-(cyclopropylamino)ethyl]-6-methoxypyridin-2-yl}prop-2-en-1-yl)carbamate[Ex(298-2)] as a colorless oil.

APCI-MS m/z: 306 [M+H]⁺.

3) Methyl((2E)-3-{4-[1-(cyclopropylamino)ethyl]-6-methoxypyridin-2-yl}prop-2-en-1-yl)carbamateand (2R)-4-(tert-butoxycarbonyl)morpholine-2-carboxylic acid weretreated in the similar manner to Example 162 to give tert-butyl(2R)-2-({cyclopropyl[1-(2-methoxy-6-{(1E)-3-[(methoxycarbonyl)amino]prop-1-en-1-yl}pyridin-4-yl)ethyl]amino}carbonyl)morpholine-4-carboxylate[Ex(298-3)] as a colorless oil.

APCI-MS m/z: 519 [M+H]⁺.

4) tert-Butyl(2R)-2-({cyclopropyl[1-(2-methoxy-6-(1E)-3-[(methoxycarbonyl)amino]-prop-1-en-1-yl}pyridin-4-yl)ethyl]amino}carbonyl)morpholin-4-carboxylatewas reduced in the similar manner to Example 296(5) to give tert-butyl(2R)-2-({cyclopropyl[1-(2-methoxy-6-{3-[(methoxycarbonyl)amino]propyl}pyridin-4-yl)ethyl]amino}carbonyl)morpholin-4-carboxylate[Ex(298-4)] as a colorless oil.

APCI-MS m/z: 521 [M+H]⁺.

Examples 299 to 308 were synthesized according to a combination of themethods described herein and conventional methods.

TABLE 71 EX. No. Structural formula a b c 299

2HCl 465 [M + H]⁺ 300

2HCl 465 [M + H]⁺ 301

2HCl 463 [M + H]⁺ 302

2HCl 463 [M + H]⁺ 303

2HCl 449 [M + H]⁺

Ex. No.: Example Number b: Mass spectrometric value

a: Salt c: Ion species

TABLE 72 EX. No. Structural formula a b c 304

2HCl 449 [M + H]⁺ 305

2HCl 483 [M + H]⁺ 306

2HCl 483 [M + H]⁺ 307

2HCl 483 [M + H]⁺ 308

2HCl 483 [M + H]⁺

Ex. No.: Example Number

a: Salt

b: Mass spectrometric value

c: Ion species

Reference Example 1 N-[1-(2-Naphthyl)ethyl]cyclopropylaminehydrochloride [REx(1-2)]

(1) N-Cyclopropyl-N-[1-(2-naphthyl)ethyl]-2-nitrobenzenesulfonamide[REx(1-1)]:

To a solution of 1-(2-naphthyl)ethanol (344 mg),N-cyclopropyl-2-nitrobenzenesulfonamide (581 mg) and triphenylphosphine(787 mg) in tetrahydrofuran (10 mL) was added dropwise diisopropylazodicarboxylate (590 μL) under ice-cooling, and the mixture was stirredat room temperature for 3 hours. The reaction solution was concentrated,and the resulting residue was purified by silica gel columnchromatography (eluent: n-hexane/ethyl acetate=4/1→41/1), and thentriturated with diethyl ether-n-hexane (1:1) to giveN-cyclopropyl-N-[1-(2-naphthyl)ethyl]-2-nitrobenzenesulfonamide[REx(1-1)] (499 mg) as a colorless powder.

APCI-MS m/z: 397 [M+H]⁺.

(2) N-[1-(2-Naphthyl)ethyl]cyclopropylamine hydrochloride [REx(1-2)]:

To a solution of the compound obtained in (1) (480 mg) and4-bromothiophenol (250 mg) in N,N-dimethylformamide (12 mL) was addedpotassium carbonate (304 mg), and the mixture was stirred at roomtemperature for 17 hours. To the reaction solution was added water, andthe mixture was extracted with ethyl acetate. The organic layer wassequentially washed with water twice and saturated saline, dried oversodium sulfate, and then concentrated under reduced pressure. Theresulting residue was dissolved in ethyl acetate (5 mL), and thenthereto was added 4-normal hydrogen chloride-ethyl acetate (1 mL). Theprecipitated solid was filtered to giveN-[1-(2-naphthyl)ethyl]cyclopropylamine hydrochloride [REx(1-2)] (211mg) as a colorless powder.

APCI-MS m/z: 212 [M+H]⁺.

Reference Example 2

(1) 3-Methoxypropyl 4-fluoro-3-(3-methoxypropoxy)benzoate [REx(2-1)]:

To a solution of 4-fluoro-3-hydroxybenzoic acid (2.0 g) in acetonitrile(100 mL)-N,N-dimethylformamide (50 mL)-water (2.0 mL) were addedpotassium carbonate (5.31 g) and 1-bromo-3-methoxypropane (4.32 g), andthe mixture was heated to reflux at 90° C. for 18 hours. To the reactionmixture was added water under ice-cooling, and then the mixture wasextracted with ethyl acetate. The organic layer was washed withsaturated saline, dried over magnesium sulfate, and then concentratedunder reduced pressure. The resulting residue was purified by silica gelcolumn chromatography (eluent: n-hexane/ethyl acetate=4/1→1/2 to give3-methoxypropyl 4-fluoro-3-(3-methoxypropoxy)benzoate [REx(2-1)] (2.72g) as a colorless oil.

APCI-MS m/z: 301 [M+H]⁺.

(2) [Fluoro-3-(3-methoxypropoxy)phenyl]methanol [REx(2-2)]:

To a suspension of lithium aluminum hydride (344 mg) in tetrahydrofuran(20 mL) was added dropwise a solution of the compound obtained in theabove (1) (2.72 g) in tetrahydrofuran (8 mL) under ice-cooling, and thenthe mixture was stirred under the cooling for 1 hour. Under the cooling,to the reaction mixture were sequentially and slowly added water and2-normal aqueous sodium hydroxide solution (1 mL), and then the mixturewas stirred at room temperature for 1 hour. An insoluble was filteredoff through Celite, and the filtrate was washed with aqueous saturatedsodium hydrogen carbonate solution, and then dried over magnesiumsulfate. The resultant was concentrated under reduced pressure, and theresulting residue was purified by silica gel column chromatography(eluent: n-hexane/ethyl acetate=2/1→1/2) to give[fluoro-3-(3-methoxypropoxy)phenyl]methanol [REx(2-2)] (1.78 g) as acolorless oil.

APCI-MS m/z: 232[M+NH₄]⁺.

(3) 4-Fluoro-3-(3-methoxypropoxy)benzaldehyde [REx(2-3)]:

To a solution of the compound obtained in the above (2) (1.65 g) indichloromethane (43 mL) was added 85% activated manganese dioxide (7.88g), and the mixture was stirred at room temperature for 1 hour, and thenthe mixture was heated to reflux for 2 hours. An insoluble was filteredoff through Celite, and then the filtrate was concentrated under reducedpressure to give 4-fluoro-3-(3-methoxypropoxy)benzaldehyde [REx(2-3)](1.59 g) as a colorless oil.

APCI-MS m/z: 213 [M+H]⁺.

(4) 1-[4-Fluoro-3-(3-methoxypropoxy)phenyl]ethanol [REx(2-4)]:

To a solution of the compound obtained in the above (3) (1.55 g) intetrahydrofuran (30 mL) was added dropwise a solution of methylmagnesiumbromide in 3M diethyl ether (2.68 mL) under ice-cooling, and the mixturewas stirred at the same temperature for 10 minutes. Under ice-cooling,thereto was added aqueous ammonium chloride solution, and the mixturewas extracted with ethyl acetate. The organic layer was sequentiallywashed with water and saturated saline, and then dried over magnesiumsulfate and concentrated under reduced pressure to give1-[4-fluoro-3-(3-methoxypropoxy)phenyl]ethanol [REx(2-4)] (1.43 g) as ayellow oil.

APCI-MS m/z: 246 [M+NH₄]⁺.

(5) Then, an amine compound [REx(2-6)] may be obtained in the similarmanner to Reference Example 1.

Reference Example 3

(1) tert-Butyl {[4-(benzyloxy)-2-naphthyl]methyl}cyclopropylcarbamate[REx(3-1)]:

To a solution of N-[4-(benzyloxy)-2-naphthyl]methyl}cyclopropylamine(12.3 g) in dichloromethane (150 mL) were added triethylamine (5.93 mL)and di-t-butyl dicarbonate (9.29 g) under ice-cooling, and the mixturewas stirred at room temperature for 3 hours. To the reaction solutionwas added saturated aqueous ammonium chloride solution underice-cooling, and the mixture was extracted with chloroform. The organiclayer was washed with water and saturated saline, dried over sodiumsulfate, and then concentrated under reduced pressure. The resultingresidue was purified by silica gel column chromatography (eluent:n-hexane/ethyl acetate=19/1→9/1) to give tert-butyl{[4-(benzyloxy)-2-naphthyl]methyl}cyclopropylcarbamate [REx(3-1)] (15.8g) as a colorless powder.

APCI-MS m/z: 404 [M+H]⁺.

(2) tert-Butyl {1-[4-(benzyloxy)-2-naphthyl]ethyl}cyclopropylcarbamate[REx(3-2)]:

To a solution of the compound obtained in the above (1) (807 mg) andtetramethylethylenediamine (0.39 μL) in tetrahydrofuran (10 mL) wereadded dropwise a solution of 1.55M n-butyllithium in hexane (1.55 mL) at−78° C. under argon over 5 minutes. The mixture was stirred at the sametemperature for 1 hour, and then thereto was added iodomethane (0.187μL) at −78° C. The mixture was stirred at the same temperature for 30minutes, and then stirred under ice-cooling for 2 hours. To the reactionsolution was added saturated aqueous ammonium chloride solution underice-cooling, and then the mixture was extracted with ethyl acetate. Theorganic layer was washed with water twice and saturated saline, driedover sodium sulfate, and then concentrated under reduced pressure. Theresulting residue was purified by silica gel column chromatography(eluent: n-hexane/ethyl acetate=20/1→6/1) to give tert-butyl{1-[4-(benzyloxy)-2-naphthyl]ethyl}cyclopropylcarbamate [REx(3-2)] (611mg) as a colorless oil.

APCI-MS m/z: 418 [M+H]⁺.

(3) tert-Butyl[1-(4-hydroxy2-naphthyl)ethyl]carbamate [REx(3-3)]:

To a solution of the compound obtained in the above (2) (126 mg) inmethanol (3 mL) was added 10% palladium on carbon (13 mg), and themixture was stirred under hydrogen for 3 hours. The reaction solutionwas diluted with ethyl acetate, and a catalyst was filtered, and thenthe resultant was concentrated under reduced pressure. The resultingresidue was triturated with isopropyl ether/n-hexane (1:1) to givetert-butyl cyclopropyl[1-(4-hydroxy2-naphthyl)ethyl]carbamate [REx(3-3)](50 mg) as a colorless powder.

ESI-MS m/z: 326[M−H]⁻.

(4) Methyl{2-[(3-{1-[(tert-butoxycarbonyl)(cyclopropyl)amino]ethyl}-1-naphthyl)oxy]ethyl}carbamate[REx(3-4)]:

To a solution of the compound obtained in the above (3) (243 mg) andmethyl (2-bromoethyl)carbamate (203 mg) in acetonitrile (10 mL) wasadded potassium carbonate (205 mg), and the mixture was stirred at 80°C. for 7 hours. After cooling, to the reaction solution was added water,and the mixture was extracted with ethyl acetate. The organic layer waswashed with saturated saline, dried over sodium sulfate, and thenconcentrated under reduced pressure. The resulting residue was purifiedby silica gel column chromatography (eluent: n-hexane/ethylacetate=4/1→1/1) to give methyl{2-[(3-{1-[(tert-butoxycarbonyl)(cyclopropyl)amino]ethyl}-1-naphthyl)oxy]ethyl}carbamate[REx(3-4)] (161 mg) as a pale yellow oil.

APCI-MS m/z: 429 [M+H]⁺.

(5)Methyl[2-({3-[1-(cyclopropylamino)ethyl]-1-naphthyl}oxy)ethyl]carbamate[REx(3-5)]:

To a solution of the compound obtained in the above (4) (156 mg) inchloroform (2 mL) was added 4-normal hydrogen chloride-dioxane solution(2 mL) under ice-cooling, and the mixture was stirred at roomtemperature for 1 hour. The mixture was concentrated under reducedpressure, and to the resulting residue was added aqueous saturatedsodium hydrogen carbonate solution, and the mixture was extracted withchloroform. The organic layer was dried over sodium sulfate, and thenconcentrated under reduced pressure. The resulting residue was purifiedby silica gel column chromatography (eluent: ethyl acetate→ethylacetate/methanol=10/1) to give methyl[2-({3-[1-(cyclopropylamino)ethyl]-1-naphthyl}oxy)ethyl]carbamate[REx(3-5)] (76 mg) as a pale yellow oil.

APCI-MS m/z: 329 [M+H]⁺.

Reference Example 4 [1-(4-Methoxy-2-naphthyl)ethyl]cyclopropylamine[REx(4-1)]

To a mixture of tert-butylcyclopropyl[1-(4-hydroxy2-naphthyl)ethyl]carbamate (43 mg) and potassiumcarbonate (27 mg) was added N,N-dimethylformamide (2.0 mL), and thenthereto added methyl iodide (0.016 mL), and the mixture was stirred atroom temperature for 4 hours. After cooling, to the reaction solutionwas added water, and the mixture was extracted with ethyl acetate. Theorganic layer was sequentially washed with water twice and saturatedsaline, dried over sodium sulfate, and then concentrated under reducedpressure. The resulting residue was purified by silica gel columnchromatography (eluent: n-hexane/ethyl acetate=10/1→4/1) to givetert-butyl cyclopropyl[1-(4-methoxy-2-naphthyl)ethyl]carbamate[REx(4-1)] (33 mg) as a colorless oil.

APCI-MS m/z: 342 [M+H]⁺.

Then, deprotection of Boc group according to any one of methods ofExamples 1 to 5 may give the desired amine compound.

Reference Example 5

(1)tert-Butyl[3-(benzyloxy)-5-(3-methoxypropoxy)benzyl]cyclopropylcarbamate[REx(5-1)]:

To a solution ofN-[3-(benzyloxy)-5-(3-methoxypropoxy)benzyl]cyclopropylamine (15.4 g) indichloromethane (190 mL) were added triethylamine (6.60 mL) anddi-t-butyl dicarbonate (10.3 g) under ice-cooling, and the mixture wasstirred at room temperature for 4 hours. To the reaction solution wasadded saturated aqueous ammonium chloride solution under ice-cooling,and the mixture was extracted with chloroform. The organic layer waswashed with water and saturated saline, dried over sodium sulfate, andthen concentrated under reduced pressure. The resulting residue waspurified by silica gel column chromatography (eluent: n-hexane/ethylacetate=14/1) to give tert-butyl[3-(benzyloxy)-5-(3-methoxypropoxy)benzyl]cyclopropylcarbamate[REx(5-1)] (20.0 g) as a colorless oil.

APCI-MS m/z: 459[M+NH4]⁺.

(2) tert-Butyl cyclopropyl[3-hydroxy5-(3-methoxypropoxy)benzyl]carbamate[REx(5-2)]:

To a solution of the compound obtained in the above (1) (14.0 g) inethanol (210 mL) was added 20% palladium hydroxide on carbon (2.80 g),and the mixture was stirred under hydrogen for 30 minutes. An insolublewas filtered, and then concentrated under reduced pressure. Theresulting residue was purified by silica gel column chromatography(eluent: n-hexane/ethyl acetate=4/1→2/1) to give tert-butylcyclopropyl[3-hydroxy5-(3-methoxypropoxy)benzyl]carbamate [REx(5-2)](11.0 g) as a colorless oil.

APCI-MS m/z: 352 [M+H]⁺.

(3) tert-Butylcyclopropyl[3-methoxy-5-(3-methoxypropoxy)benzyl]carbamate [REx(5-3)]:

To a solution of the compound obtained in the above (2) (3.51 g) inN,N-dimethylformamide (50 mL) was added potassium carbonate (2.07 g),and then thereto was added iodomethane (0.75 mL) under ice-cooling, andthe mixture was stirred at room temperature for 23 hours. To thereaction solution was added water, and the mixture was extracted withethyl acetate. The organic layer was sequentially washed with watertwice and saturated saline, dried over sodium sulfate, and thenconcentrated under reduced pressure.

The resulting residue was purified by silica gel column chromatography(eluent: n-hexane/ethyl acetate=3/1) to give tert-butylcyclopropyl[3-methoxy-5-(3-methoxypropoxy)benzyl]carbamate [REx(5-3)](3.65 g) as a colorless oil.

APCI-MS m/z: 366 [M+H]⁺.

(4) Methylation according to the method of Reference Example 3(2), thendeprotecting Boc group according to any one of methods of Examples 1 to5 give the desired amine compound [REx(5-5)].

Reference Example 6

(1) Methyl 1-(3-methoxypropyl)-1H-indole-6-carboxylate [REx(6-1)]:

To a solution of methyl 1H-indole-6-carboxylate (5.0 g) inN,N-dimethylformamide (40 mL) was added drop by drop 60% oil-basedsodium hydride (1.37 g) under ice-cooling, and then the mixture wasstirred at room temperature for 15 minutes. Then, thereto was addeddropwise a solution of 1-bromo-3-methoxypropane (5.24 g) inN,N-dimethylformamide (10 mL) under ice-cooling, and then thereto wasadded potassium iodide (948 mg), and the mixture was stirred at roomtemperature for 3 hours. To the reaction mixture was sequentially addedethyl acetate and water under ice-cooling, and the organic layer wasseparated. The organic layer was washed with water twice and saturatedsaline, dried over sodium sulfate, and then concentrated under reducedpressure. The resulting residue was purified by silica gel columnchromatography (eluent: n-hexane/ethyl acetate=9/1→4/1) to give methyl1-(3-methoxypropyl)-1H-indole-6-carboxylate [REx(6-1)] (5.8 g) as acolorless oil.

APCI-MS m/z: 248 [M+H]⁺.

(2) Methyl 3-chloro-1-(3-methoxypropyl)-1H-indole-6-carboxylate[REx(6-2)]:

To a solution of the compound obtained in the above (1) (2.78 g) indichloromethane (35 mL) was added N-chlorosuccinimide (1.65 g) underice-cooling, and the mixture was stirred at room temperature for 18hours. To the reaction mixture was added water, and the mixture wasextracted with chloroform. The organic layer was washed with saturatedsaline, dried over sodium sulfate, and then concentrated under reducedpressure. The resulting residue was purified by silica gel columnchromatography (eluent: n-hexane→n-hexane/ethyl acetate=2/1) to givemethyl 3-chloro-1-(3-methoxypropyl)-1H-indole-6-carboxylate [REx(6-2)](3.10 g) as a yellow oil.

APCI-MS m/z: 282/284 [M+H]⁺.

(3) 3-Chloro-1-(3-methoxypropyl)-1H-indole-6-carboxylic acid [REx(6-3)]:

To a solution of the compound obtained in the above (2) (1.20 g) inethanol (10 mL) was added 2-normal aqueous sodium hydroxide solution(4.26 mL) under ice-cooling, and the mixture was stirred at roomtemperature for 20 hours. The reaction mixture was concentrated, andthen the mixture was acidified by adding 2-normal hydrochloric acidunder ice-cooling, and then thereto was added water, and the mixture wasextracted with ethyl acetate. The organic layer was washed withsaturated saline, and then concentrated under reduced pressure to give3-chloro-1-(3-methoxypropyl)-1H-indole-6-carboxylic acid [REx(6-3)](1.14 g) as a colorless powder.

ESI-MS m/z: 266/268[M−H]⁻.

(4)3-Chloro-N-methoxy-1-(3-methoxypropyl)-N-methyl-1H-indole-6-carboxamide[REx(6-4)]:

To a solution of the compound obtained in the above (3) (1.14 g),N,O-dimethylhydroxyamine hydrochloride (831 mg),1-ethyl-3-(3-dimethylaminopropyl)-carbodiimide hydrochloride (1.25 g)and 1-hydroxybenzotriazole (863 mg) in chloroform (12 mL) was addeddiisopropylethylamine (1.85 mL) under ice-cooling, and then the mixturewas stirred at room temperature for 24 hours. To the reaction mixturewas added aqueous saturated sodium hydrogen carbonate solution underice-cooling, and the mixture was extracted with chloroform. The organiclayer was sequentially washed with water and saturated saline, driedover magnesium sulfate, and then concentrated under reduced pressure.The resulting residue was purified by silica gel column chromatography(eluent: n-hexane/ethyl acetate=1/1→1/3) to give3-chloro-N-methoxy-1-(3-methoxypropyl)-N-methyl-1H-indole-6-carboxamide[REx(6-4)] (1.20 g) as a pale yellow oil.

APCI-MS m/z: 311/313 [M+H]⁺.

(5) 1-[3-Chloro-1-(3-methoxypropyl)-1H-indol-6-yl]ethanone [REx(6-5)]:

To a solution of the compound obtained in the above (4) (1.20 g) intetrahydrofuran (15 mL) was added dropwise a 3M solution ofmethylmagnesium bromide in diethyl ether (2.56 mL) under ice-cooling,and the mixture was stirred at the same temperature for 1 hour. To thereaction solution was added 1-normal hydrochloric acid underice-cooling, and the mixture was extracted with ethyl acetate. Theorganic layer was washed with saturated saline, and then dried overmagnesium sulfate and concentrated under reduced pressure. The resultingresidue was purified by silica gel column chromatography (eluent:n-hexane/ethyl acetate=4/1→1/1) to give1-[3-chloro-1-(3-methoxypropyl)-1H-indol-6-yl]ethanone [REx(6-5)] (945mg) as a pale yellow oil.

APCI-MS m/z: 266/268 [M+H]⁺.

(6)N-{1-[3-Chloro-1-(3-methoxypropyl)-1H-indol-6-yl]ethyl}cyclopropanamine[REx(6-6)]:

To a solution of the compound obtained in the above (5) (155 mg) andcyclopropylamine (99.9 mg) in dichloroethane (3.0 mL) were addedmagnesium sulfate (351 mg), sodium triacetoxyborohydride (371 mg) andacetic acid (105 mg), and then the mixture was stirred at roomtemperature for 17 hours. To the reaction mixture was added aqueoussaturated sodium hydrogen carbonate solution under ice-cooling, and themixture was extracted with chloroform. The organic layer was washed withsaturated saline, dried over magnesium sulfate, and then concentratedunder reduced pressure. The resulting residue was purified by silica gelcolumn chromatography (eluent: ethyl acetate→ethylacetate/methanol=20/1) to giveN-{1-[3-chloro-1-(3-methoxypropyl)-1H-indol-6-yl]ethyl}cyclopropanamine[REx(6-6)] (111 mg) as a pale yellow oil.

APCI-MS m/z: 307/309 [M+H]⁺.

Reference Example 7

(1) Ethyl 4-(acetyloxy)benzofuran-6-carboxylate:

To a suspension of 60% oil-based sodium hydride (6.50 g) intetrahydrofuran (400 mL) was added dropwise a solution of 4-tert-butyl1-ethyl 2-(diethoxyphosphoryl)succinate (55.0 g) in tetrahydrofuran (100mL) under ice-cooling over 30 minutes, and then the mixture was stirredunder the cooling for 1 hour. Then, thereto was added a solution of2-furaldehyde (12.8 mL) in tetrahydrofuran (40 mL) under ice-coolingover 15 minutes, and the mixture was stirred at room temperature for 1hour. Ice water was poured into the reaction mixture under ice-cooling,and the mixture was extracted with ethyl acetate. The organic layer wassequentially washed with water and saturated saline, dried over sodiumsulfate, and then concentrated under reduced pressure to give4-tert-butyl 1-ethyl (2E)-2-(2-furylmethylene)succinate (47.0 g) as abrown oil crude. Then, the oil (47.0 g) was stirred in trifluoroaceticacid (100 mL) at room temperature for 1 hour, and then concentratedunder reduced pressure. The resulting residue was treated azeotropicallywith toluene several times to give(3E)-3-(ethoxycarbonyl)-4-(2-furyl)-but-3-enoic acid (39.2 g) as a brownoil crude. Then, the oil (39.2 g) was dissolved in acetic anhydride (100mL), and thereto was added potassium acetate (19.8 g), and then themixture was heated to reflux for 45 minutes. The reaction mixture waslet stand to be cooled, and then thereto was added water (100 mL), andthen the mixture was extracted with ethyl acetate. The organic layer waswashed with saturated saline, dried over sodium sulfate, and thenconcentrated under reduced pressure. The resulting residue was purifiedby silica gel column chromatography (eluent: n-hexane/ethyl acetate=5/1)to give ethyl 4-(acetyloxy)benzofuran-6-carboxylate (24.7 g) as a paleorange solid. APCI-MS m/z: 266[M+NH₄]⁺.

(2) Ethyl 4-hydroxy-1-benzofuran-6-carboxylate [REx(7-1)]:

To a solution of the compound obtained in the above (1) (24.7 g) inethanol (150 mL) was added potassium carbonate (42.0 g), and the mixturewas heated to reflux for 30 minutes. The reaction mixture wasice-cooled, and then acidified by 10% hydrochloric acid, and thenextracted with ethyl acetate. The organic layer was washed withsaturated saline, dried over sodium sulfate, and then concentrated underreduced pressure. The resulting residue was triturated withn-hexane-dichloromethane (5:1) to give ethyl4-hydroxy-1-benzofuran-6-carboxylate [REx(7-1)] (19.6 g) as a paleyellow powder.

APCI-MS m/z: 207 [M+H]⁺.

(3) Ethyl 4-(3-methoxypropoxy)-1-benzofuran-6-carboxylate [REx(7-2)]:

To a solution of the compound obtained in the above (2) (5.0 g) inacetonitrile (50 mL) were added potassium carbonate (5.0 g) and1-bromo-3-methoxypropane (4.54 g), and the mixture was heated to refluxfor 1.5 hours. To the reaction mixture was added water underice-cooling, and then the mixture was extracted with ethyl acetate. Theorganic layer was washed with saturated saline, dried over sodiumsulfate, and then concentrated under reduced pressure. The resultingresidue was purified by silica gel column chromatography (eluent:n-hexane/ethyl acetate=5/1→2/1) to give ethyl4-(3-methoxypropoxy)-1-benzofuran-6-carboxylate [REx(7-2)] (6.61 g) as acolorless oil.

APCI-MS m/z: 279 [M+H]⁺.

(4) 4-(3-Methoxypropoxy)-1-benzofuran-6-carboxylic acid [REx(7-3)]:

To a solution of the compound obtained in the above (3) (2.64 g) inethanol (20 mL) was added 2-normal aqueous sodium hydroxide solution(9.5 mL), and the mixture was stirred at room temperature for 3 hours.Then, thereto was added 2-normal hydrochloric acid under ice-cooling,and the mixture was extracted with ethyl acetate. The organic layer waswashed with saturated saline, dried over magnesium sulfate, and thenconcentrated under reduced pressure to give4-(3-methoxypropoxy)-1-benzofuran-6-carboxylic acid [REx(7-3)] (2.40 g)as a colorless powder.

APCI-MS m/z: 265 [M+H+MeOH—H₂O]⁺.

(5) N-Methoxy-4-(3-methoxypropoxy)-N-methyl-1-benzofuran-6-carboxamide[REx(7-4)]:

To a solution of the compound obtained in the above (4) (2.39 g),N,O-dimethylhydroxyamine.hydrochloride (1.86 g),1-ethyl-3-(3-dimethylaminopropyl)carbodiimide hydrochloride (2.74 g) and1-hydroxybenzotriazole (1.93 g) in chloroform (30 mL) was addeddiisopropylethylamine (4.2 mL) under ice-cooling, and the mixture wasstirred at room temperature for 4 hours. Under ice-cooling, to thereaction mixture was added aqueous saturated sodium hydrogen carbonatesolution, and the mixture was extracted with chloroform. The organiclayer was sequentially washed with water and saturated saline, driedover magnesium sulfate, and then concentrated under reduced pressure.The resulting residue was purified by silica gel column chromatography(eluent: n-hexane/ethyl acetate=1/1→1/3) to giveN-methoxy-4-(3-methoxypropoxy)-N-methyl-1-benzofuran-6-carboxamide[REx(7-4)] (2.67 g) as a pale yellow oil.

APCI-MS m/z: 294 [M+H]⁺.

(6) 1-[4-((3-Methoxypropoxy)-1-benzofuran-6-yl]ethanone [REx(7-5)]:

To a solution of the compound obtained in the above (5) (2.67 g) intetrahydrofuran (30 mL) was added dropwise a 3M solution ofmethylmagnesium bromide in diethyl ether (6.1 mL) under ice-cooling, andthe mixture was stirred at the same temperature for 15 minutes. Underice-cooling, 10% hydrochloric acid was poured into the mixture, and thenthe mixture was extracted with ethyl acetate. The organic layer waswashed with saturated saline, and then dried over magnesium sulfate andconcentrated under reduced pressure. The resulting residue was purifiedby silica gel column chromatography (eluent: n-hexane/ethylacetate=4/1→1/1) to give1-[4-((3-methoxypropoxy)-1-benzofuran-6-yl]ethanone [REx(7-5)] (2.15 g)as a colorless powder.

APCI-MS m/z: 249 [M+1-1]⁺.

(7) N-{1-[4-(3-Methoxypropoxy)-1-benzofuran-6-yl]ethyl}cyclopropanamine[REx(7-6)]:

To a solution of the compound obtained in the above (6) (2.15 g) andcyclopropylamine (2.10 mL) in dichloroethane (150 mL) were added sodiumtriacetoxyborohydride (5.50 g), acetic acid (1.48 mL) and magnesiumsulfate (5.20 g), and then the mixture was stirred at room temperaturefor 23 hours. Thereto was added aqueous saturated sodium hydrogencarbonate solution under ice-cooling, and the mixture was extracted withchloroform. The organic layer was washed with saturated saline, driedover magnesium sulfate, and then concentrated under reduced pressure.The resulting residue was purified by silica gel column chromatography(eluent: chloroform/methanol=20/1→13/1) to giveN-{1-[4-(3-methoxypropoxy)-1-benzofuran-6-yl]ethyl}cyclopropanamine[REx(7-6)] (2.47 g) as a pale yellow oil.

APCI-MS m/z: 290 [M+H]⁺.

Reference Example 8

(1) 1-(6-Methyl-1H-pyrrolo[2,3-b]pyridin-3-yl)ethanone [REx(8-1)]:

To a solution of 6-methyl-1H-pyrrolo[2,3-b]pyridine (500 mg) indichloroethane (6 mL) were added aluminum chloride (1.09 g) and acetylchloride (0.40 mL), and then the mixture was stirred at room temperaturefor 1.5 hours. The reaction solution was poured into aqueous saturatedsodium hydrogen carbonate solution, and extracted with chloroform. Theorganic layer was washed with saturated saline, dried over magnesiumsulfate, and then concentrated under reduced pressure. The resultingresidue was triturated with isopropyl ether to give1-(6-methyl-1H-pyrrolo[2,3-b]pyridin-3-yl)ethanone [REx(8-1)] (481 mg)as a yellow powder.

APCI-MS m/z: 175 [M+H]⁺.

(2)1-[1-(4-Methoxybutyl)-6-methyl-1H-pyrrolo[2,3-b]pyridin-3-yl]ethanone[REx(8-2)]:

To a solution of the compound obtained in the above (1) (280 mg) inN,N-dimethylformamide (6 mL) was added 60% oil-based sodium hydride(83.6 mg), and then the mixture was stirred at room temperature for 30minutes. Thereto was added dropwise a solution of 4-methoxybutyl4-methylbenzenesulfonate in N,N-dimethylformamide (1 mL), and thenthereto was added potassium iodide (267 mg), and the mixture was stirredat 50° C. for 1 hour. The reaction mixture was poured into water, andextracted with ethyl acetate. The organic layer was washed with watertwice and saturated saline, dried over magnesium sulfate, and thenconcentrated under reduced pressure. The resulting residue was purifiedby silica gel column chromatography (eluent: n-hexane/ethylacetate=2/1→1/9) to give1-[1-(4-methoxybutyl)-6-methyl-1H-pyrrolo[2,3-b]pyridin-3-yl]ethanone[REx(8-2)] (366 mg) as a yellow oil.

APCI-MS m/z: 261 [M+H]⁺.

(3) An amine compound [REx(8-3)] is obtained in the similar manner toReference Example 7(7).

Reference Example 91-[1-(3-Methoxypropyl)-3-methyl-1H-indol-6-yl]ethanone [REx(9-1)]

To a solution of 1-[3-chloro-1-(3-methoxypropyl)-1H-indol-6-yl]ethanone(843 mg) in 1,4-dioxane (15 mL) were added potassium phosphate (1.35 g),trimethylboroxine (883 mg), tris(dibenzylideneacetone)dipalladium (290mg) and 2-dicyclohexylphosphino-2′,4′,6′-triisopropyl-1,1′-biphenyl(X-Phos) (605 mg) under argon, and the mixture was heated to stir at110° C. for 4 hours. Thereto was added water under ice-cooling, and themixture was extracted with ethyl acetate. The organic layer was washedwith saturated saline, dried over magnesium sulfate, and thenconcentrated under reduced pressure. The resulting residue was purifiedby silica gel column chromatography (eluent: n-hexane/ethylacetate=3/1→3/2) to give1-[1-(3-methoxypropyl)-3-methyl-1H-indol-6-yl]ethanone [REx(9-1)] (663mg) as a yellow oil.

APCI-MS m/z: 246 [M+H]⁺.

Reference Example 10 Methyl 1-(3-methoxypropyl)indoline-6-carboxylate[REx(10-1)]

To a mixture of methyl 1-(3-methoxypropyl)-1H-indole-6-carboxylate (1.5g) and sodium cyanohydroborate (1.61 g) was added acetic acid (15 mL)under ice-cooling, and the mixture was stirred at room temperature for 3hours. To the reaction solution was added aqueous saturated sodiumhydrogen carbonate solution under ice-cooling, and the mixture wasneutralized by adding sodium hydrogen carbonate, and then extracted withethyl acetate. The organic layer was washed with saturated saline, driedover sodium sulfate, and then concentrated under reduced pressure. Theresulting residue was purified by silica gel column chromatography(eluent: n-hexane→n-hexane/ethyl acetate=2/1) to give methyl1-(3-methoxypropyl)indoline-6-carboxylate [REx(10-1)] (1.20) as a paleyellow oil.

APCI-MS m/z: 250 [M+H]⁺.

Reference Example 11 6-(3-Methoxypropoxy)indan-1-one [REx(11-1)]

To a solution of 6-hydroxyindan-1-one (1.48 g) in acetonitrile (30 mL)were added 4-methoxybutyl 4-methylbenzenesulfonate (2.93 g), potassiumiodide (166 mg) and potassium carbonate (2.07 g), and the mixture washeated to stir at 80° C. for 5 hours. To the reaction mixture was addedwater under ice-cooling, and then the mixture was extracted with ethylacetate. The organic layer was washed with saturated saline, dried oversodium sulfate, and then concentrated under reduced pressure. Theresulting residue was purified by silica gel column chromatography(eluent: n-hexane/ethyl acetate=7/3), and then triturated withn-hexane/isopropyl ether (2:1) to give 6-(3-methoxypropoxy)indan-1-one[REx(11-1)] (1.1 g) as a colorless powder.

APCI-MS m/z: 221 [M+H]⁺.

Reference Example 127-(3-Methoxypropoxy)-3,4-dihydronaphthalen-1(2H)-one [REx(12-1)]

To a solution of 7-hydroxy-1-tetralone (4.05 g) in acetonitrile (75 mL)were added 1-bromo-3-methoxypropane (4.59 g), potassium iodide (415 mg)and potassium carbonate (5.18 g), and the mixture was heated to stir at80° C. for 23 hours. To the reaction mixture was added water underice-cooling, and then the mixture was extracted with ethyl acetate. Theorganic layer was washed with saturated saline, dried over sodiumsulfate, and then concentrated under reduced pressure. The resultingresidue was triturated with n-hexane to give7-(3-methoxypropoxy)-3,4-dihydronaphthalen-1(2H)-one [REx(12-1)] (4.80g) as a colorless powder.

APCI-MS m/z: 235 [M+H]⁺.

Reference Example 13 N-(2-Methoxyethyl)-3-oxoindan-5-carboxamide[REx(13-1)]

To a suspension of 1-indanone-6-carboxylic acid (1.76 g) inN,N-dimethylformamide (50 mL) was added carbonyldiimidazole (3.24 g),and the mixture was stirred at room temperature for 1 hour. Thereto wasadded a solution of 2-methoxyethylamine (3.76 g) in dichloromethane (50mL) under ice-cooling, and the mixture was stirred at room temperaturefor 4 hours. The reaction solution was concentrated under reducedpressure, and then dissolved in chloroform. The organic layer was washedwith aqueous saturated sodium hydrogen carbonate solution, dried oversodium sulfate, and then concentrated under reduced pressure. Theresulting residue was dissolved in tetrahydrofuran (20 mL), and thenthereto was added 1-normal hydrochloric acid (20 mL), and the mixturewas stirred at room temperature for 12 hours. To the reaction solutionwas added water, and the mixture was extracted with ethyl acetate. Theorganic layer was washed with saturated saline, dried over sodiumsulfate, and then concentrated under reduced pressure. The resultingresidue was triturated with isopropyl ether-ethyl acetate (2:1) to giveN-(2-methoxyethyl)-3-oxoindan-5-carboxamide [REx(13-1)] (1.87 g) as abrown powder.

APCI-MS m/z: 234 [M+H]⁺.

Reference Example 14

(1) Methyl 3-iodo-4-methyl-5-nitrobenzoate [REx(14-1)]:

To a suspension of methyl 3-amino-4-methyl-5-nitrobenzoate (36.0 g) in6-normal hydrochloric acid (276 mL) was added dropwise a solution ofsodium nitrite (13.0 g) in water (35 mL) under ice-salt-cooling over 20minutes, and the mixture was stirred under ice-cooling for 1 hour. Then,thereto was added dropwise a solution of potassium iodide (34.1 g) inwater (280 mL) under ice-cooling over 20 minutes, and the mixture wasstirred at room temperature for 2 hours. To the reaction solution wasadded water under ice-cooling, and the mixture was extracted withchloroform. The organic layer was sequentially washed with aqueoussaturated sodium thiosulfate solution and saturated saline, dried oversodium sulfate, and then concentrated under reduced pressure. Theresulting residue was purified by silica gel column chromatography(eluent: n-hexane/ethyl acetate=50/1) to give methyl3-iodo-4-methyl-5-nitrobenzoate [REx(14-1)] (40.5 g) as a yellow powder.

(2) Methyl 3-amino-5-iodo-4-methylbenzoate [REx(14-2)]:

To a solution of the compound obtained in the above (1) (40.5 g) inethyl acetate (500 mL) was added tin (II) chloride dihydrate (142 g),and the mixture was heated to stir at 60° C. for 1 hour. Aqueous sodiumhydrogen carbonate solution was poured into the reaction mixture underice-cooling, and then an insoluble was filtered through Celite. Theorganic layer was separated, and then washed with saturated saline,dried over sodium sulfate, and then concentrated under reduced pressureto give methyl 3-amino-5-iodo-4-methylbenzoate [REx(14-2)] (35.3 g) as apale yellow powder.

APCI-MS m/z: 292 [M+H]⁺.

(3) Methyl 4-iodo-1H-indazole-6-carboxylate [REx(14-3)]:

To a suspension of the compound obtained in the above (2) (35.3 g) inwater (615 mL) were added concentrated hydrochloric acid (102 mL) andammonium tetrafluoroborate (16.5 g), and the mixture was cooled to −3°C. Under the cooling, thereto was added dropwise a solution of sodiumnitrite (9.20 g) in water (34 mL) over 20 minutes. The mixture wasstirred at −3° C. for 1 hour, and then the precipitated crystal wasfiltered, sequentially washed with water (100 mL) and diethyl ether (100mL), and then dried under reduced pressure. The resulting solid wassuspended in chloroform (420 mL), and then thereto were added potassiumacetate (13.1 g) and 18-crown-6 (801 mg), and the mixture was stirred atroom temperature for 15 hours. To the reaction mixture was added waterunder ice-cooling, and then the mixture was extracted with chloroform.The organic layer was washed with saturated saline, dried over sodiumsulfate, and then concentrated under reduced pressure. The resultingresidue was triturated with chloroform to give methyl4-iodo-1H-indazole-6-carboxylate [REx(14-3)] (18.9 g) as a pale orangepowder.

APCI-MS m/z: 303 [M+H]⁺.

(4) Methyl 3-bromo-4-iodo-1H-indazole-6-carboxylate [REx(14-4)]:

The compound obtained in the above (3) (24.5 g) was dissolved in aceticacid (720 mL), and after blocking out light, bromine (8.30 mL) was addeddropwise to the mixture at room temperature. After stirring at roomtemperature for 40 hours, bromine (4.15 mL) was added thereto, and themixture was stirred for additional 24 hours at room temperature. Then,thereto were added acetic acid (100 mL) and bromine (4.15 mL), and themixture was stirred at room temperature for 6 hours. The reactionmixture was poured into ice water, and then thereto was added sodiumthiosulfate, and the mixture was stirred at room temperature for 20minutes, and then the precipitated solid was filtered. The solid waswashed with water, and then dissolved in ethyl acetate and washed withsaturated saline. The organic layer was dried over sodium sulfate, andthen concentrated under reduced pressure. The resulting residue wastriturated with isopropyl ether to give methyl3-bromo-4-iodo-1H-indazole-6-carboxylate [REx(14-4)] (27.3 g) as a paleyellow powder.

APCI-MS m/z: 381/383 [M+H]⁺.

(5) Methyl 3-bromo-4-iodo-1-(3-methoxypropyl)-1H-indazole-6-carboxylate[REx(14-5)]:

To a solution of the compound obtained in the above (4) (22.3 g) inN,N-dimethylformamide (200 mL) was added 60% oil-based sodium hydride(2.81 g) under ice-cooling, and the mixture was stirred at roomtemperature for 15 minutes. To the mixture was added a solution of1-bromo-3-methoxypropane (10.8 g) in N,N-dimethylformamide (40 mL) underice-cooling, and the mixture was stirred at room temperature for 18hours. 10% Hydrochloric acid was poured into the reaction solution underice-cooling, and then the mixture was extracted with ethyl acetate. Theorganic layer was sequentially washed with water and saturated saline,dried over sodium sulfate, and then concentrated under reduced pressure.The resulting residue was purified by silica gel column chromatography(eluent: n-hexane/ethyl acetate=9/1) to give methyl3-bromo-4-iodo-1-(3-methoxypropyl)-1H-indazole-6-carboxylate [REx(14-5)](23.5 g) as a pale orange powder.

APCI-MS m/z: 453/455 [M+H]⁺.

Reference Example 151-[4-Chloro-1-(3-methoxypropyl)-3-methyl-1H-indazol-6-yl]ethanone[REx(15-1)]

To a solution of1-[3-bromo-4-chloro-1-(3-methoxypropyl)-1H-indazol-6-yl]ethanone (1.0 g)in 1,4-dioxane (20 mL) were added potassium carbonate (1.2 g),trimethylboroxine (0.41 mL) and 1,1′-bis(diphenylphosphino)ferrocenedichloropalladium (II) (212 mg) under argon, and the mixture was heatedto stir at 80° C. for 24 hours. Then, thereto was added water underice-cooling, and the mixture was extracted with ethyl acetate. Theorganic layer was washed with saturated saline, dried over magnesiumsulfate, and then concentrated under reduced pressure. The resultingresidue was purified by NH-silica gel column chromatography (eluent:n-hexane/ethyl acetate=4/1→3/2) to give1-[4-chloro-1-(3-methoxypropyl)-3-methyl-1H-indazol-6-yl]ethanone[REx(15-1)] (392 mg) as a pale yellow oil.

APCI-MS m/z: 281/283 [M+H]⁺.

Reference Example 161-[1-(3-Methoxypropyl)-3,4-dimethyl-1H-indazol-6-yl]ethanone [REx(16-1)]

To a solution of1-[3-bromo-4-chloro-1-(3-methoxypropyl)-1H-indazol-6-yl]ethanone (1.0 g)in 1,4-dioxane (7.5 mL) were added potassium carbonate (1.2 g),trimethylboroxine (1.0 mL) and 1,1′-bis(diphenylphosphino)ferrocenedichloropalladium (II) (212 mg) under argon, and the mixture was heatedto stir at 110° C. for 24 hours. Then, thereto was added water underice-cooling, and the mixture was extracted with ethyl acetate. Theorganic layer was washed with saturated saline, dried over magnesiumsulfate, and then concentrated under reduced pressure. The resultingresidue was purified by NH-silica gel column chromatography (eluent:n-hexane/ethyl acetate=3/1→1/1) to give1-[1-(3-methoxypropyl)-3,4-dimethyl-1H-indazol-6-yl]ethanone [REx(16-1)](689 mg) as an orange oil.

APCI-MS m/z: 261 [M+H]⁺.

Reference Example 171-[3-Bromo-1-(3-methoxypropyl)-4-(trifluoromethyl)-1H-indazol-6-yl]ethanone[REx(17-1)]

A mixture of1-[3-bromo-4-iodo-1-(3-methoxypropyl)-1H-indazol-6-yl]ethanone (500 mg),methyl fluorosulfonyldifluoroacetate (1.36 g), hexamethylphosphorylamide(1.27 g) and copper (I) iodide (337 mg) was heated to stir inN,N-dimethylformamide (7.0 mL) under argon at 75° C. for 15 hours. Waterand ethyl acetate were poured into the reaction mixture underice-cooling, and then an insoluble was filtered through Celite. Theorganic layer was separated, and then sequentially washed with water andsaturated saline, dried over sodium sulfate, and then concentrated underreduced pressure. The resulting residue was purified by silica gelcolumn chromatography (eluent: n-hexane→n-hexane/ethyl acetate=1/1) togive1-[3-bromo-1-(3-methoxypropyl)-4-(trifluoromethyl)-1H-indazol-6-yl]ethanone[REx(17-1)] (87 mg) as a yellow oil.

APCI-MS m/z: 379/381 [M+H]⁺.

Reference Example 18

(1) Methyl 1-(3-methoxypropyl)-1H-indazole-6-carboxylate [REx(18-1)]:

To a solution of methyl3-bromo-1-(3-methoxypropyl)-1H-indazole-6-carboxylate (3.0 g) anddiisopropylethylamine (2.4 mL) in methanol (60 mL) was added 10%palladium on carbon catalyst (600 mg), and the mixture was stirred underhydrogen for 1 hour. An insoluble was filtered, and then the filtratewas concentrated under reduced pressure. The residue was dissolved inchloroform, sequentially washed with 10% hydrochloric acid water andsaturated saline, and then concentrated under reduced pressure to givemethyl 1-(3-methoxypropyl)-1H-indazole-6-carboxylate [REx(18-1)] (2.40g) as a pale yellow oil.

(2) Methyl 3-fluoro-1-(3-methoxypropyl)-1H-indazole-6-carboxylate[REx(18-2)]:

To a solution of the compound obtained in the above (1) (2.20 g) inacetonitrile (30 mL) was added Selectfluor (Registered trademark) (3.45g), and the mixture was stirred at 80° C. for 15 hours. Then, theretowas added aqueous sodium hydrogen carbonate solution under ice-cooling,and the mixture was extracted with ethyl acetate. The organic layer waswashed with saturated saline, dried over sodium sulfate, and thenconcentrated under reduced pressure. The resulting residue was purifiedby silica gel column chromatography (eluent: n-hexane→n-hexane/ethylacetate=5/1) to give methyl3-fluoro-1-(3-methoxypropyl)-1H-indazole-6-carboxylate [REx(18-2)] (1.01g) as a colorless oil.

APCI-MS m/z: 267 [M+H]⁺.

Reference Example 19 1-[3-Fluoro-5-(3-methoxypropoxy)phenyl]ethanone[REx(19-2)]

1-Bromo-3-fluoro-5-(3-methoxypropoxy)benzene (4.0 g) was added to water(30.4 mL), and then thereto were added ethylene glycol monovinyl ether(6.8 mL), potassium carbonate (2.52 g),1,3-bis(diphenylphosphino)propane (125 mg) and palladium acetate (34mg), and the mixture was heated to stir at 90° C. for 22 hours. Aftercooling, thereto was added concentrated hydrochloric acid (7.2 mL), andthe mixture was stirred at room temperature for 20 minutes. The reactionsolution was extracted with ethyl acetate, washed with saturated saline,and then dried over magnesium sulfate. After concentrating under reducedpressure, the resulting residue was purified by silica gel columnchromatography (eluent: n-hexane/ethyl acetate=20/1→2/1) to give1-[3-fluoro-5-(3-methoxypropoxy)phenyl]ethanone [REx(19-2)] (1.03 g) asa yellow oil.

APCI-MS m/z: 227 [M+H]⁺.

A starting material is prepared from 1-bromo-3-fluoro-phenol, forexample, in the similar manner to Reference Example 11 or 12.

Reference Example 20 1-[3-Hydroxy-5-(3-methoxypropoxy)phenyl]ethanone[REx(20-1)]

To a solution of 1-(3,5-dihydroxyphenyl)ethanone (10 g) inN,N-dimethylformamide (164 mL)-water (5 mL) were added potassiumcarbonate (13.6 g) and 3-methoxypropyl 4-methylbenzene sulfonate (16.1g), and the mixture was heated to stir at 80° C. for 2 hours. Thereaction solution was concentrated under reduced pressure, and thenthereto was added water, and then the mixture was extracted with ethylacetate. The organic layer was washed with saturated saline, dried oversodium sulfate, and then concentrated under reduced pressure. Theresulting residue was purified by silica gel column chromatography(eluent: n-hexane/ethyl acetate=3/1→1/1) to give1-[3-hydroxy-5-(3-methoxypropoxy)phenyl]ethanone [REx(20-1)] (4.65 g) asa colorless powder and 1-[3,5-bis(3-methoxypropoxy)phenyl]ethanone[REx(20-2)] (4.98 g) as a colorless oil.

2: APCI-MS m/z: 225 [M+H]⁺.

3: APCI-MS m/z: 297 [M+H]⁺.

Reference Example 21

(1) 3-Acetyl-5-(3-methoxypropoxy)phenyltrifluoromethanesulfonate[REx(21-1)]:

To a solution of 1-[3-hydroxy-5-(3-methoxypropoxy)phenyl]ethanone (4.65g) in chloroform (100 mL) was added pyridine (5.02 mL) underice-cooling, and then thereto was added dropwisetrifluoromethanesulfonic anhydride (3.67 mL) under the ice-cooling, andthen the mixture was stirred at the same temperature for 20 minutes.Then, thereto was added 1-normal hydrochloric acid, and the mixture wasextracted with chloroform, and then the organic layer was sequentiallywashed with water and saturated saline, dried over magnesium sulfate,and then concentrated under reduced pressure to give a crude product of3-acetyl-5-(3-methoxypropoxy)phenyltrifluoromethanesulfonate [REx(21-1)](8.22 g) as a yellow oil.

APCI-MS m/z: 374[M+NH₄]⁺.

(2) 1-[3-(3-Methoxypropoxy)-5-methylphenyl]ethanone [REx(21-2)]:

To a solution of the compound obtained in the (1) (8.22 g) in1,4-dioxane (100 mL) were added potassium carbonate (8.6 g),trimethylboroxine (3.5 mL) and 1,1′-bis(diphenylphosphino)ferrocenedichloropalladium (II) (1.51 g), and the mixture was heated to stir at110° C. for 2 hours. After cooling to room temperature, thereto wasadded water, and the mixture was extracted with ethyl acetate. Theorganic layer was washed with saturated saline, dried over magnesiumsulfate, and then concentrated under reduced pressure. The resultingresidue was purified by silica gel column chromatography (eluent:n-hexane→n-hexane/ethyl acetate=5/1) to give1-[3-(3-methoxypropoxy)-5-methylphenyl]ethanone [REx(21-2)] (4.15 g) asa brown oil.

APCI-MS m/z: 223 [M+H]⁺.

Reference Example 22

(1) 1-(4-Chloro-3,5-dimethoxyphenyl)ethanol [REx(22-1)]:

To a solution of 4-chloro-3,5-dimethoxybenzaldehyde (1.0 g) intetrahydrofuran (20 mL) was added dropowise a 3M solution ofmethylmagnesium bromide in diethyl ether (1.83 mL) under ice-cooling,and the mixture was stirred at the same temperature for 1 hour. Then,thereto was added aqueous ammonium chloride solution under ice-cooling,and the mixture was extracted with ethyl acetate. The organic layer wassequentially washed with water and saturated saline, and then dried overmagnesium sulfate and concentrated under reduced pressure to give acrude product of 1-(4-chloro-3,5-dimethoxyphenyl)ethanol [REx(22-1)](1.23 g) as a colorless powder.

APCI-MS m/z: 200 [M+H—H₂O]⁺.

(2) 1-(4-Chloro-3,5-dimethoxyphenyl)ethanone [REx(22-2)]:

To a solution of the compound obtained in the above (1) (1.23 g) indichloromethane (28 mL) was added 85% activated manganese dioxide (5.81g), and the mixture was stirred at 40° C. for 4 hours. The reactionsolution was cooled to room temperature, and then thereto was addedwater-chloroform, and an insoluble was filtered off through Celite, andthen the organic layer was separated. The organic layer was concentratedunder reduced pressure, and the resulting residue was purified by silicagel column chromatography (eluent: n-hexane/ethyl acetate=10/1→1/1) togive 1-(4-chloro-3,5-dimethoxyphenyl)ethanone [REx(22-2)] (723 mg) as acolorless powder.

APCI-MS m/z: 215 [M+H]⁺.

Reference Example 23

(1) 6-(1-Ethoxyvinyl)-1-(3-methoxypropyl)-1H-pyrrolo[3,2-b]pyridine[REx(23-2)]:

To a solution of 6-bromo-1-(3-methoxypropyl)-1H-pyrrolo[3,2-b]pyridine(1.5 g) in toluene (30 mL) were added tri-n-butyltin-1-ethoxyvinyl (5.65mL) and dichlorobis-(triphenylphosphine)palladium (II) (782 mg), and themixture was heated to stir at 110° C. for 30 minutes. The reactionsolution was cooled to room temperature, and then thereto was addedwater-ethyl acetate, and an insoluble was filtered through Celite. Theorganic layer was separated, and then washed with saturated saline,dried over magnesium sulfate, and then concentrated under reducedpressure. The resulting residue was purified by NH-silica gel columnchromatography (eluent: n-hexane/ethyl acetate=4/1→1/1) to give6-(1-ethoxyvinyl)-1-(3-methoxypropyl)-1H-pyrrolo[3,2-b]pyridine[REx(23-2)] (1.69 g) as a yellow oil.

A starting compound [REx(23-1)] is obtained by 3-methoxypropylation at Nof 6-bromo-1-1H-pyrrolo[3,2-b]pyridine.

APCI-MS m/z: 261 [M+H]⁺.

(2) 1-[1-(3-Methoxypropyl)-1H-pyrrolo[3,2-b]pyridin-6-yl]ethanone[REx(23-3)]:

The compound obtained in the above (1) (1.69 g) was dissolved inchloroform (20 mL), and then thereto was added 4-normal hydrogenchloride/1,4-dioxane under ice-cooling, and the mixture was stirred atthe same temperature for 2 hours. Then, thereto was added aqueoussaturated sodium hydrogen carbonate solution under ice-cooling, and themixture was extracted with chloroform. The organic layer was washed withsaturated saline, dried over magnesium sulfate, and then concentratedunder reduced pressure. The resulting residue was purified by NH-silicagel column chromatography (eluent: n-hexane/ethyl acetate=2/1→1/1) togive 1-[1-(3-methoxypropyl)-1H-pyrrolo[3,2-b]pyridin-6-yl]ethanone[REx(23-3)] (766 mg) as a yellow oil.

APCI-MS m/z: 233 [M+H]⁺.

Reference Example 24 1-[4-Methoxy-3-(4-methoxybutyl)phenyl]ethanone[REx(24-1)]

To a solution of 4-bromo-1-methoxy-2-(4-methoxybutyl)benzene (523 mg) intetrahydrofuran (10 mL) were added lithium chloride (326 mg),tetrakis(triphenylphosphine)palladium (0) (381 mg) andtri-n-butyltin-1-ethoxyvinyl (1.11 mL), and the mixture was heated tostir at 80° C. for 20 hours. The reaction solution was cooled to roomtemperature, and then thereto was added aqueous potassium fluoridesolution, and the mixture was stirred for 30 minutes. The mixture wasextracted with diethyl ether, and then thereto was added 10%hydrochloric acid, and the mixture was stirred for 1 hour. The organiclayer was separated, dried over magnesium sulfate, and then concentratedunder reduced pressure. The resulting residue was purified by silica gelcolumn chromatography (eluent: n-hexane/ethyl acetate=9/1→1/1) to give1-[4-methoxy-3-(4-methoxybutyl)phenyl]ethanone [REx(24-1)] (195 mg) as ayellow oil.

APCI-MS m/z: 237 [M+H]⁺.

Reference Example 25

(1) tert-Butyl1-(3-methoxypropyl)-1H-pyrrolo[3,2-c]pyridine-6-carboxylate [REx(25-1)]:

To a solution of tert-butyl 1H-pyrrolo[3,2-c]pyridine-6-carboxylate (2.0g) in N,N-dimethylformamide (15 mL) was added drop by drop 60% oil-basedsodium hydride (385 mg) under ice-cooling, and then the mixture wasstirred at room temperature for 15 minutes. Then, thereto was addeddropwise a solution of 1-bromo-3-methoxypropane (1.47 g) inN,N-dimethylformamide (5 mL) under ice-cooling, and then the mixture wasstirred at room temperature for 18 hours. To the reaction mixture wasadded water under ice-cooling, and the mixture was extracted with ethylacetate. The organic layer was sequentially washed with water andsaturated saline, dried over sodium sulfate, and then concentrated underreduced pressure. The resulting residue was purified by silica gelcolumn chromatography (eluent: n-hexane/ethyl acetate=1/1) to givetert-butyl 1-(3-methoxypropyl)-1H-pyrrolo[3,2-c]pyridine-6-carboxylate[REx(25-1)] (2.52 g) as a yellow oil.

APCI-MS m/z: 291 [M+H]⁺.

(2) tert-Butyl3-chloro-1-(3-methoxypropyl)-1H-pyrrolo[3,2-c]pyridine-6-carboxylate[REx(25-2)]:

To a solution of the compound obtained in the above (1) (2.52 g) indichloromethane (50 mL) was added N-chlorosuccinimide (1.50 g) underice-cooling, and the mixture was stirred at room temperature for 72hours. To the reaction mixture was added water under ice-cooling, andthe mixture was extracted with chloroform. The organic layer was washedwith saturated saline, dried over sodium sulfate, and then concentratedunder reduced pressure. The resulting residue was purified by NH-silicagel column chromatography (eluent: n-hexane/ethyl acetate=9/1) to givetert-butyl3-chloro-1-(3-methoxypropyl)-1H-pyrrolo[3,2-c]pyridine-6-carboxylate[REx(25-2)] (2.45 g) as a colorless powder.

APCI-MS m/z: 325/327 [M+H]⁺.

(3) 3-Chloro-1-(3-methoxypropyl)-1H-pyrrolo[3,2-c]pyridine-6-carboxylicacid. hydrochloride [REx(25-3)]:

The compound obtained in the above (2) (2.42 g) was added totrifluoroacetic acid (24 mL), and the mixture was stirred at roomtemperature for 2 hours. To the reaction solution was added 1-normalhydrochloric acid water (15 mL) under ice-cooling, and then the mixturewas concentrated under reduced pressure. The resulting residue wastriturated with isopropyl ether to give3-chloro-1-(3-methoxypropyl)-1H-pyrrolo[3,2-c]pyridine-6-carboxylic acidhydrochloride [REx(25-3)] (2.20 g) as a brown powder.

ESI-MS m/z: 267/269[M−H]⁻.

Reference Example 26

(1) 1-[4-(Benzyloxy)-3-hydroxyphenyl]ethanone [REx(26-1)]:

To a solution of 3′,4′-dihydroxyacetophenone (25.4 g) inN,N-dimethylacetamide (420 mL) were added potassium carbonate (23.1 g)and benzyl bromide (19.9 mL) under ice-cooling, and the mixture wasstirred at room temperature for 90 minutes. An insoluble was filtered,and then diluted with ethyl acetate. The organic layer was sequentiallywashed with water and saturated saline, dried over magnesium sulfate,and then concentrated under reduced pressure. The resulting residue waspurified by silica gel column chromatography (eluent:n-hexane→n-hexane/ethyl acetate=20/1), and then triturated with ethylacetate to give 1-[4-(benzyloxy)-3-hydroxyphenyl]ethanone [REx(26-1)](11.0 g) as a colorless powder.

APCI-MS m/z: 243 [M+H]⁺.

(2) 1-[4-(Benzyloxy)-3-(3-methoxypropyl)phenyl]ethanone [REx(26-2)]:

To a solution of the compound obtained in the above (1) (11.0 g) inacetonitrile (113 mL) were added potassium carbonate (9.37 g) and3-methoxypropyl 4-methylbenzene sulfonate (13.2 g), and the mixture washeated to reflux for 20 hours. The reaction solution was cooled to roomtemperature, and then thereto was added water, and the mixture wasextracted with ethyl acetate. The organic layer was dried over magnesiumsulfate, and then concentrated under reduced pressure. The resultingresidue was triturated with diisopropyl ether to give1-[4-(benzyloxy)-3-(3-methoxypropyl)phenyl]ethanone [REx(26-2)] (8.75 g)as a colorless powder.

APCI-MS m/z: 315 [M+H]⁺.

Reference Example 27

(1) 4-Bromo-2-(3-methoxypropoxy)benzoic acid [REx(27-1)]:

To a solution of 3-methoxy-1-propanol (5.02 g) in N,N-dimethylformamide(37 mL) was added 60% oil-based sodium hydride (2.05 g), and the mixturewas stirred at room temperature for 30 minutes. Then, thereto was addeddropwise a solution of 4-bromo-2-fluorobenzoic acid (300 mg) inN,N-dimethylformamide (60 mL), and the mixture was stirred at roomtemperature for 20 hours. To the reaction solution was added water andn-hexane, and then the mixed solution was acidified by concentratedhydrochloric acid. The resulting colorless powder was filtered to give4-bromo-2-(3-methoxypropoxy)benzoic acid [REx(27-1)] (4.43 g).

ESI-MS m/z: 289[M−H]—.

(2) Methyl 4-bromo-2-(3-methoxypropoxy)benzoate [REx(27-2)]:

To a mixture of the compound obtained in the above (1) (4.42 g) andpotassium carbonate (4.22 g) was added N,N-dimethylformamide (20 mL),and then thereto was added methyl iodide (1.43 mL), and the mixture wasstirred at room temperature for 30 minutes. After cooling, to thereaction solution was added water, and the mixture was extracted withethyl acetate. The organic layer was sequentially washed with watertwice and saturated saline, dried over magnesium sulfate, and thenconcentrated under reduced pressure. The resulting residue was purifiedby silica gel column chromatography (eluent: n-hexane/ethylacetate=20/1→3/1) to give methyl 4-bromo-2-(3-methoxypropoxy)benzoate[REx(27-2)] (4.01 g) as a colorless oil.

APCI-MS m/z: 343/305 [M+H]⁺.

(3) Methyl 4-acetyl-2-(3-methoxypropoxy)benzoate [REx(27-3)]:

To a solution of the compound obtained in the above (2) (4.0 g) intoluene (44 mL) were added tri-n-butyltin-1-ethoxyvinyl (8.90 mL) anddichlorobis(triphenylphosphine)palladium (II) (1.85 g), and the mixturewas heated to stir at 100° C. for 17 hours. The reaction solution wascooled to room temperature, and then thereto was added 4-normal hydrogenchloride-1,4-dioxane (24 mL), and the mixture was stirred at roomtemperature for 1 hour. To the reaction solution was added water, andthe mixture was extracted with ethyl acetate. The organic layer waswashed with saturated saline, and then thereto were added magnesiumsulfate and NH-silica gel, and an insoluble was filtered. The filtratewas concentrated under reduced pressure. The resulting residue waspurified by silica gel column chromatography (eluent: n-hexane/ethylacetate=20/1) to give methyl 4-acetyl-2-(3-methoxypropoxy)benzoate[REx(27-3)] (1.02 g) as a yellow oil.

APCI-MS m/z: 267 [M+H]⁺.

Reference Example 28

(1) N-[(1E)-1H-Pyrrolo[2,3-b]pyridin-3-ylmethylene]cyclopropylamine[REx(28-1)]:

To a suspension of 1H-pyrrolo[2,3-b]pyridin-3-carbaldehyde (1.46 g) inethanol (30 mL) was added cyclopropylamine (1.41 mL), and the mixturewas stirred at 50° C. for 19 hours. The reaction solution wasconcentrated under reduced pressure, and then treated azeotropicallywith toluene. The resulting residue was triturated with isopropylether/n-hexane (3:1) to giveN-[(1E)-1H-pyrrolo[2,3-b]pyridin-3-ylmethylene]cyclopropylamine[REx(28-1)] (1.75 g) as a colorless powder.

APCI-MS m/z: 186 [M+H]⁺.

(2) N-[1-(1H-Pyrrolo[2,3-b]pyridin-3-yl)ethyl]cyclopropylamine[REx(28-2)]:

To a suspension of the compound obtained in the above (1) (1.11 g) and1-(trimethylsilyl)-1H-benzotriazole (2.20 mL) in toluene (50 mL) wasadded dropwise a 3M solution of methylmagnesium bromide in diethyl ether(10 mL) under ice-cooling over 10 minutes, and the mixture was stirredat 110° C. for 6 hours. The reaction solution was poured into ice-cooledammonium chloride solution, and extracted with ethyl acetate. Aninsoluble was filtered, and then the organic layer was separated, andthe aqueous layer was extracted with chloroform. The organic layers werecollected, dried over sodium sulfate, and then concentrated underreduced pressure. The resulting residue was dissolved in ethyl acetate,and extracted with 10% aqueous citric acid solution. The aqueous layerwas alkalified by aqueous potassium carbonate solution, and thenextracted with chloroform. The organic layer was dried over sodiumsulfate, and then concentrated under reduced pressure to give a crudeproduct of N-[1-(1H-pyrrolo[2,3-b]pyridin-3-yl)ethyl]cyclopropylamine[REx(28-2)] (800 mg) as a yellow oil.

(3) tert-Butylcyclopropyl[1-(1H-pyrrolo[2,3-b]pyridin-3-yl)ethyl]carbamate[REx(28-3)]:

To a solution of the compound obtained in the above (2) (800 mg) andpotassium carbonate (1.10 g) in tetrahydrofuran (10 mL)-water (10 mL)was added a solution of di-t-butyl dicarbonate (786 mg) intetrahydrofuran (1 mL), and the mixture was stirred at room temperaturefor 6 hours. To the reaction solution was added water, and the mixturewas extracted with ethyl acetate. The organic layer was sequentiallywashed with water and saturated saline, dried over sodium sulfate, andthen concentrated under reduced pressure. The resulting residue wasdissolved in acetonitrile (20 mL), and then thereto were added sodiumhydroxide (320 mg) and tetrabutylammonium hydrogen sulfate (68 mg), andthe mixture was stirred at 50° C. for 30 minutes. The reaction solutionwas cooled, and then an insoluble was filtered, and the filtrate wasconcentrated under reduced pressure. The resulting residue was dissolvedin ethyl acetate, and washed with water. The organic layer was driedover sodium sulfate, and then concentrated under reduced pressure. Theresulting residue was purified by silica gel column chromatography(eluent: n-hexane/ethyl acetate=3/1→1/3) to give tert-butylcyclopropyl[1-(1H-pyrrolo[2,3-b]pyridin-3-yl)ethyl]carbamate [REx(28-3)](338 mg) as a pale yellow oil.

APCI-MS m/z: 302 [M+H]⁺.

(4) tert-Butyl1-[1-(3-aminopropyl)-1H-pyrrolo[2,3-b]pyridin-3-yl]ethyl}-cyclopropylcarbamate[REx(28-4)]:

To a solution of the compound obtained in the above (3) (301 mg) inacetonitrile (10 mL) were added sodium hydroxide (300 mg) andtetrabutylammonium hydrogen sulfate (17 mg), and the mixture was stirredat room temperature for 15 minutes. Then, thereto was added3-chloropropylamine hydrochloride (650 mg), and the mixture was stirredat 70° C. for 4 hours. The reaction solution was cooled, and then aninsoluble was filtered, and the filtrate was concentrated under reducedpressure. The resulting residue was dissolved in ethyl acetate, andsequentially washed with water and saturated saline. The organic layerwas dried over sodium sulfate, and then concentrated under reducedpressure to give a crude product of tert-butyl{1-[1-(3-aminopropyl)-1H-pyrrolo[2,3-b]pyridin-3-yl]ethyl}cyclopropylcarbamate[REx(28-4)] (378 mg) as a yellow oil.

APCI-MS m/z: 359 [M+H]⁺.

(5) Methyl[3-(3-{1-[(tert-butoxycarbonyl)(cyclopropyl)amino]ethyl}-1H-pyrrolo[2,3-b]pyridin-1-yl)propyl]carbamate[REx(28-5)]:

To a solution of the compound obtained in the above (4) (370 mg) inchloroform (10 mL) were added pyridine (0.25 mL) and methylchloroformate (0.16 mL) under ice-cooling, and the mixture was stirredat room temperature for 2 hours. The reaction solution was concentrated,and then treated azeotropically with toluene. The resulting residue wasdissolved in chloroform, and washed with 1-normal aqueous sodiumhydrogen carbonate solution. The organic layer was dried over sodiumsulfate, and then concentrated under reduced pressure. The resultingresidue was purified by silica gel column chromatography (eluent:n-hexane/ethyl acetate=1/1→ethyl acetate) to give methyl[3-(3-{1-[(tert-butoxycarbonyl)-(cyclopropyl)amino]ethyl}-1H-pyrrolo[2,3-b]pyridin-1-yl)propyl]carbamate[REx(28-5)] (203 mg) as a colorless oil.

APCI-MS m/z: 417 [M+H]⁺.

(6) Methyl(3-{3-[1-(cyclopropylamino)ethyl]-1H-pyrrolo[2,3-b]pyridin-1-yl}propyl)carbamate[REx(28-6)]:

To a solution of the compound obtained in the above (5) (187 mg) and2,6-lutidine (0.157 mL) in dichloromethane (4 mL) was addedtrimethylsilyltriflate (0.180 μL) under ice-cooling, and the mixture wasstirred at the same temperature for 1 hour. Then, thereto were addedaqueous saturated sodium hydrogen carbonate solution and methanol (2 mL)under ice-cooling, and the mixture was extracted with chloroform. Theorganic layer was dried over magnesium sulfate, and then concentratedunder reduced pressure. The resulting residue was purified by silica gelcolumn chromatography (eluent:chloroform→chloroform/methanol=5/1→chloroform/methanol/ammoniawater=50/10/1) to give methyl(3-{3-[1-(cyclopropylamino)ethyl]-1H-pyrrolo[2,3-b]pyridin-1-yl}propyl)carbamate[REx(28-6)] (89 mg) as a colorless oil.

Reference Example 29

(1) tert-ButylN-{1-[3-bromo-4-iodo-1-(3-methoxypropyl)-1H-indazol-6-yl]ethyl}-cyclopropylcarbamate[REx(29-1)]:

To a solution ofN-{1-[3-bromo-4-iodo-1-(3-methoxypropyl)-1H-indazol-6-yl]ethyl}cyclopropanamine(10.2 g) in dichloromethane (200 mL) was added di-t-butyl dicarbonate(5.12 g) under ice-cooling, and the mixture was stirred at roomtemperature for 21 hours. Then, thereto was added dimethylaminopyridine(261 mg), and the mixture was stirred for additional 6 hours at roomtemperature. To the reaction solution was added water under ice-cooling,and the mixture was extracted with chloroform. The organic layer waswashed with saturated saline, dried over sodium sulfate, and thenconcentrated under reduced pressure. The resulting residue was purifiedby silica gel column chromatography (eluent: n-hexane/ethylacetate=9/1→4/1) to give tert-butylN-{1-[3-bromo-4-iodo-1-(3-methoxypropyl)-1H-indazol-6-yl]ethyl}cyclopropylcarbamate[REx(29-1)] (7.62 g) as a yellow oil.

(2) tert-Butyl1-[3-bromo-4-(3,5-dimethylisoxazol-4-yl)-1-(3-methoxypropyl)-1H-indazol-6-yl]ethyl}-cyclopropylcarbamate[REx(29-2)]:

To a solution of the compound obtained in the above (1) (300 mg) and3,5-dimethylisoxazol-4-boronic acid (146 mg) in dimethoxyethane (5.0 mL)was added 2M aqueous sodium carbonate solution (2.6 mL) under argon, andthen thereto was added tetrakis(triphenylphosphine)palladium (0) (30mg), and the mixture was stirred at 105° C. for 22 hours. Then, theretowas added water under ice-cooling, and the mixture was extracted withethyl acetate. The organic layer was washed with saturated saline, driedover sodium sulfate, and then concentrated under reduced pressure. Theresulting residue was purified by silica gel column chromatography(eluent: n-hexane→n-hexane/ethyl acetate=3/2) to give tert-butyl1-[3-bromo-4-(3,5-dimethylisoxazol-4-yl)-1-(3-methoxypropyl)-1H-indazol-6-yl]ethyl}-cyclopropylcarbamate[REx(29-2)] (154 mg) as a colorless oil.

APCI-MS m/z: 547/549 [M+H]⁺.

Deprotection of Boc group is done according to the above method.

Reference Example 30 tert-Butyl cyclopropyl1-[1-(3-methoxypropyl)-1H-indazol-6-yl]ethyl}carbamate [REx(30-1)]

To a solution of tert-butylN-{1-[3-bromo-4-iodo-1-(3-methoxypropyl)-1H-indazol-6-yl]ethyl}cyclopropylcarbamate(1.0 g) in 1,4-dioxane (20 mL) were added diisopropylethylamine (0.90mL) and 10% palladium on carbon catalyst (200 mg), and the mixture wasstirred under hydrogen for 42 hours. An insoluble was filtered, and thenthe filtrate was sequentially washed with aqueous saturated sodiumhydrogen carbonate solution and saturated saline, dried over sodiumsulfate, and then concentrated under reduced pressure. The resultingresidue was purified by silica gel column chromatography (eluent:n-hexane/ethyl acetate=911-411) to give tert-butyl cyclopropyl1-[1-(3-methoxypropyl)-1H-indazol-6-yl]ethyl}carbamate [REx(30-1)] (167mg) as a colorless oil.

APCI-MS m/z: 374 [M+H]⁺.

Reference Example 31N-{1-[3-(3-Methoxypropyl)-5-(trifluoromethyl)phenyl]ethyl}cyclopropanamine[REx(31-1)]

To a solution of 3-methoxy-1-propanol (0.14 mL) in N,N-dimethylformamide(3.0 mL) was added 60% oil-based sodium hydride (97 mg), and the mixturewas stirred at room temperature for 10 minutes. Then, thereto was addeddropwise a solution ofN-{1-[3-fluoro-5-(trifluoromethyl)phenyl]ethyl}cyclopropylamine (300 mg)in N,N-dimethylformamide (1.0 mL), and the mixture was heated to stir at40° C. for 4 hours. After cooling to room temperature, thereto was addedwater, and the mixture was extracted with ethyl acetate. The organiclayer was sequentially washed with water and saturated saline, driedover magnesium sulfate, and then concentrated under reduced pressure.The resulting residue was purified by silica gel column chromatography(eluent: n-hexane/ethyl acetate=10/1→2/3) to giveN-{1-[3-(3-methoxypropyl)-5-(trifluoromethyl)phenyl]ethyl}cyclopropanamine[REx(31-1)] (227 mg) as a colorless oil.

APCI-MS m/z: 318 [M+H]⁺.

Reference Example 32

(1) Methyl4-{1-[(tert-butoxycarbonyl)(cyclopropyl)amino]ethyl}-2-(3-methoxypropoxy)benzoate[REx(32-1)]:

To a solution of methyl4-[1-(cyclopropylamino)ethyl]-2-(3-methoxypropoxy)benzoate (1.20 g) inchloroform (9.6 mL) were added di-t-butyl dicarbonate (2.00 g) andtriethylamine (2.34 mL) under ice-cooling, and the mixture was stirredat room temperature for 20 hours. To the reaction solution was addedwater under ice-cooling, and the mixture was extracted with chloroform.The organic layer was dried over magnesium sulfate, and thenconcentrated under reduced pressure. The resulting residue was purifiedby silica gel column chromatography (eluent: n-hexane/ethylacetate=10/1→2/1) to give methyl4-{1-[(tert-butoxycarbonyl)-(cyclopropyl)amino]ethyl}-2-(3-methoxypropoxy)benzoate(7.62 g) as a colorless oil.

APCI-MS m/z: 408 [M+H]⁺.

(2)4-{1-[(tert-Butoxycarbonyl)(cyclopropyl)amino]ethyl}-2-(3-methoxypropoxy)benzoicacid [REx(32-2)]:

To a solution of the compound obtained in the above (1) (1.10 g) inmethanol (13.5 mL) was added 2-normal aqueous sodium hydroxide solution(13.5 mL), and the mixture was stirred at room temperature for 2 hours.To the reaction solution was added chloroform, and then thereto wasadded 2-normal hydrochloric acid (13.5 mL) under ice-cooling. Theorganic layer was separated, and then washed with water, dried overmagnesium sulfate, and then concentrated under reduced pressure. Theresulting residue was purified by silica gel column chromatography(eluent: chloroform→chloroform/methanol=20/1) to give4-{1-[(tert-butoxycarbonyl)(cyclopropyl)amino]ethyl}-2-(3-methoxypropoxy)benzoicacid (1.14 g) as a colorless oil.

ESI-MS m/z: 392[M−H]⁻

(3) tert-butyl1-[4-(aminocarbonyl)-3-(3-methoxypropoxy)phenyl]ethyl-cyclopropylcarbamate[REx(32-3)]:

To a solution of the compound obtained in the above (2) (250 mg) inN,N-dimethylformamide (3.2 mL) were added ammonium chloride (40.8 mg),diisopropylethylamine (0.133 mL),1-ethyl-3-(3-dimethylaminopropyl)carbodiimide hydrochloride (146 mg) and1-hydroxybenzotriazole (103 mg), and then the mixture was stirred atroom temperature for 30 minutes. To the reaction mixture was addedwater, and the mixture was extracted with ethyl acetate. The organiclayer was sequentially washed with water and saturated saline, and thenconcentrated under reduced pressure. The resulting residue was purifiedby silica gel column chromatography (eluent: n-hexane/ethylacetate=3/2→1/6) to give tert-butyl{1-[4-(aminocarbonyl)-3-(3-methoxypropoxy)phenyl]ethyl}cyclopropylcarbamate(164 mg) as a colorless oil.

APCI-MS m/z: 393 [M+H]⁺.

Similarly, deprotection of Boc group is done according to the abovemethod.

Reference Example 33 2-[1-(Cyclopropylamino)ethyl]quinazolin-4(3H)-one[REx(33-1)]

To a suspension of 2-(1-bromoethyl)quinazolin-4(3H)-one (2.53 g) inN,N-dimethylformamide (30 mL) was added cyclopropylamine (3.46 mL), andthe mixture was diluted with N,N-dimethylformamide (20 mL) and water (1mL), and then stirred for 18 hours. The reaction solution wasconcentrated under reduced pressure, and to the residue was addedaqueous sodium hydrogen carbonate solution, and then the mixture wasextracted with chloroform. The organic layer was dried over sodiumsulfate, and then concentrated under reduced pressure. The resultingresidue was triturated with diisopropyl ether/ethyl acetate (20:1) togive 2-[1-(cyclopropylamino)ethyl]quinazolin-4(3H)-one [REx(33-1)] (1.71g) as a colorless powder.

APCI-MS m/z: 230 [M+H]⁺.

Reference Examples 34 to 100

The following compounds of Reference Examples 34 to 100 were preparedaccording to the methods of the above Reference Examples. Each symbol ofMethods A-1 to F refers to each method according to the followingmethods of Reference Examples.

Method A-1 Reference Example 1

Method A-2 Reference Example 2

Method B Reference Example 3

Method C-1 Reference Example 6

Method C-2 Reference Example 7

Method C-3 Reference Example 8

Method D Reference Example 28

Method E-1 Reference Example 29

Method E-2 Reference Example 30

Method E-3 Reference Example 31

Method E-4 Reference Example 32

Method F Reference Example 33

TABLE 73 Ref. EX. No. Structural formula a b c d e f 34

HCl 279.3746 P 280 [M + H]+ B 35

HCl 221.2955 P 222 [M + H]+ C-2 36

287.3999 O 288 [M + H]+ C-3 37

221.29551 O 222 [M + H]+ C-2

TABLE 74 38

235.322 O 236 [M + H]+ C-2 39

HCl 211.3022 P 212 [M + H]+ A 40

HCl 261.3593 P 262 [M + H]+ C-2 41

267.3391 O 268 [M + H]+ C-2

TABLE 75 42

263.3752 O 264 [M + H]+ A-2 43

267.3391 O 268 [M + H]+ A-2 44

263.3752 O 264 [M + H]+ C-2 45

279.3746 O 280 [M + H]+ C-2

TABLE 76 46

301.4265 O 302 [M + H]+ C-3 47

305.3904 O 306 [M + H]+ C-3 48

321.845 O 322/324 [M + H]+ C-1 49

301.4265 O 302 [M + H]+ C-1

TABLE 77 50

242.3162 P 243 [M + H]+ C--2 51

274.3581 O 275 [M + H]+ C-2 52

HCl 297.2394 P 298 [M + H]+ C-2 53

316.3981 O 317 [M + H]+ D

TABLE 78 54

241.3282 O 242 [M + H]+ B 55

328.4055 O 329 [M + H]+ B 56

317.3466 O 318 [M + H]+ E-3 57

255.7405 O 256/258 [M + H]+ C-2

TABLE 79 58

255.7405 O 256/258 [M + H]+ C-2 59

337.4537 O 338 [M + H]+ C-2 60

274.3581 O 275 [M + H]+ C-2 61

274.4011 O 275 [M + H]+ C-1

TABLE 80 62

275.3859 O 276 [M + H]+ C-2 63

289.3694 O 290 [M + H]+ C-2 64

306.8303 O 307/309 [M + H]+ C-1 65

249.3486 O 250 [M + H]+ C-2

TABLE 81 66

297.3651 O 298 [M + H]+ B 67

355.3979 O 356 [M + H]+ C-1 68

321.845 O 322/ 324 [M + H]+ C-3 69

287.3999 O 288 [M + H]+ C-3

TABLE 82 70

305.3904 O 306 [M + H]+ C-3 71

358.2707 O 358/360 [M + H]+ C-2 72

273.3733 O 274 [M + H]+ C-3 73

287.3999 O 288 [M + H]+ C-3

TABLE 83 74

286.4118 O 287 [M + H]+ C-1 75

304.3841 O 305 [M + H]+ C-2 76

289.3694 O 290 [M + H]+ C-2 77

287.3999 O 288 [M + H]+ C-1

TABLE 84 78

313.8197 O 314/316 [M + H]+ C-2 79

288.388 O 289 [M + H]+ C-3 80

301.4265 O 302 [M + H]+ C-3 81

217.3068 O 218 [M + H]+ C-2

TABLE 85 82

355.4706 O 356 [M + H]+ C-2 83

320.4265 O 321 [M + H]+ E-4 84

306.3999 O 307 [M + H]+ E-4 85

292.3734 P 293 [M + H]+ E-4

TABLE 86 86

229.2777 P 230 [M + H]+ F 87

246.7353 O 247/249 [M + H]+ C-2 88

277.4018 O 278 [M + H]+ C-2 89

273.3733 O 274 [M + H]+ C-1

TABLE 87 90

303.3993 O 304 [M + H]+ C-2 91

303.3993 O 304 [M + H]+ C-2 92

382.4992 O 383 [M + H]+ E-1 93

291.3638 O 292 [M + H]+ C-1

TABLE 88 94

315.3555 O 316 [M + H]+ C-2 95

287.3999 O 288 [M + H]+ C-1 96

307.8184 O 308/310 [M + H]+ C-1 97

273.3733 O 274 [M + H]+ E-2

TABLE 89 98

305.3904 O 306 [M + H]+ C-1 99

259.3037 O 260 [M + H]+ C-1 100

334.3885 O 335 [M + H]+ C-1

Ref Ex. No.: Reference Example Number

a: Salt

b: Molecular weight

c: Properties

d: MS Results APCI

e: Ion species

f: Method

O: Oil

P: Powder

Reference Example 101 Methyl 3-acetyl-1H-indazole-1-carboxylate

To a solution of 1-(1H-indazol-3-yl)ethanone (5.0 g) and triethylamine(6.53 mL) in chloroform (80 mL) was added dropwise a solution of methylchlorocarbonate (3.24 g) in chloroform (20 mL) under ice-cooling over 1hour, and the mixture was stirred at room temperature for 14 hours. Thereaction solution was washed with aqueous saturated sodium chloridesolution, dried over magnesium sulfate, and then concentrated underreduced pressure. The resulting residue was triturated with n-hexane togive methyl 3-acetyl-1H-indazole-1-carboxylate) [REx(101-1)] (6.67 g) asa colorless powder.

APCI-MS m/z: 219 [M+H]⁺.

Reference Example 102Methyl[3-(3-acetyl-6-fluoro-1H-indazol-1-yl)propyl]carbamate[REx(102-2)]

(1) To a solution of 6-fluoro-3-iodo-1H-indazole (1.5 g) and methyl(3-bromopropyl)carbamate (1.68 g) in N,N-dimethylformamide (5 mL) wasadded potassium carbonate (1.58 g), and the mixture was stirred at roomtemperature for 3 days. To the reaction solution was added ethylacetate, and the mixture was washed with aqueous saturated sodiumchloride solution, dried over magnesium sulfate, and then concentratedunder reduced pressure. The resulting residue was purified by silica gelcolumn chromatography (eluent: n-hexane/ethyl acetate=1/20→3/2) to givemethyl [3-(6-fluoro-3-iodo-1H-indazol-1-yl)propyl]carbamate [REx(102-1)](836 mg) as a red oil.

APCI-MS m/z: 378 [M+H]⁺.

(2) To a solution of the compound obtained in (1) (830 mg) in1,4-dioxane (10 mL) were added tri-n-butyltin-1-ethoxyvinyl (1.03 anddichlorobis(triphenylphosphine)palladium (II) (155 mg), and the mixturewas heated to reflux for 17 hours. The reaction solution was cooled toroom temperature, and then thereto was added a solution of potassiumfluoride (250 mg) in water (3 mL), and the mixture was stirred at roomtemperature for 15 minutes. Then, thereto was added 1-normalhydrochloric acid (5 mL), and the mixture was stirred at roomtemperature for 1 hour, and then an insoluble was filtered. To thefiltrate was added ethyl acetate, and the mixture was washed withaqueous saturated sodium chloride solution, dried over magnesiumsulfate, and then concentrated under reduced pressure. The resultingresidue was purified by silica gel column chromatography (eluent:n-hexane/ethyl acetate=20/1→4/1) to give methyl[3-(3-acetyl-6-fluoro-1H-indazol-1-yl)propyl]carbamate [REx(102-2)] (437mg) as a red oil.

APCI-MS m/z: 294 [M+H]⁺.

Reference Example 103

1) To 1-[1-(4-methoxybenzyl)-1H-pyrazolo[3,4-b]pyridin-3-yl]ethanone(3.14 g) was added trifluoroacetic acid (20 mL), and the mixture washeated to reflux for 2 days. The reaction solution was concentratedunder reduced pressure. The resulting residue was diluted with ethylacetate, and then sequentially washed with aqueous saturated sodiumhydrogen carbonate solution and saturated saline, dried over magnesiumsulfate, and then concentrated under reduced pressure. The resultingresidue was purified by silica gel column chromatography (eluent:n-hexane/ethyl acetate=95/5-7/3) to give1-(1H-pyrazolo[3,4-b]pyridin-3-yl)ethanone) [REx(103-1)] (1.73 g) as apale yellow powder.

APCI-MS m/z: 162 [M+H]⁺.

2) To a solution of 1-(1H-pyrazolo[3,4-b]pyridin-3-yl)ethanone (500 mg)and methyl (3-bromopropyl)carbamate (912 mg) in N,N-dimethylformamide (5mL) was added potassium carbonate (864 mg), and the mixture was stirredat room temperature for 3 days. To the reaction solution was added ethylacetate, and the mixture was washed with saturated saline, dried overmagnesium sulfate, and then concentrated under reduced pressure. Theresulting residue was purified by silica gel column chromatography(eluent: n-hexane/ethyl acetate=95/5→1/1) to give methyl[3-(3-acetyl-1H-pyrazolo[3,4-b]pyridin-1-yl)propyl]carbamate)[REx(103-2)] (308 mg) as a red oil.

APCI-MS m/z: 277 [M+H]⁺.

Reference Example 104

1) To a mixture of 2-chloro-6-methylnicotinaldehyde (5.0 g) andhydrazine monohydrate (6.24 mL) was added para-toluenesulfonic acidmonohydrate (3.67 g), and the mixture was stirred at 130° C. for 18hours. The reaction solution was cooled, and then thereto was added 10%aqueous citric acid solution, and the mixture was stirred at roomtemperature for 30 minutes. The reaction solution was extracted withethyl acetate, and washed with saturated saline. The organic layer wasdried over sodium sulfate, and then concentrated under reduced pressureto give 6-methyl-1H-pyrazolo[3,4-b]pyridine [REx(104-1)] (3.61 g) as abrown powder.

APCI-MS m/z: 134 [M+H]⁺.

2) To a solution of 6-methyl-1H-pyrazolo[3,4-b]pyridine (4.44 g) andiodine (16.9 g) in N,N-dimethylformamide (100 mL) was added potassiumhydroxide (7.48 g) under ice-cooling, and the mixture was stirred atroom temperature for 4 hours. The reaction solution was poured into icewater, and the precipitate was filtered. The filtrate was extracted withethyl acetate, washed with saturated saline, and then dried over sodiumsulfate and concentrated under reduced pressure. The resulting residuewas combined with the above-mentioned precipitate and purified by silicagel column chromatography (eluent: chloroform→chloroform/methanol=19/1)to give 3-iodo-6-methyl-1H-pyrazolo[3,4-b]pyridine [REx(104-2)] (6.48 g)as a brown powder.

APCI-MS m/z: 260 [M+H]⁺.

3) 3-Iodo-6-methyl-1H-pyrazolo[3,4-b]pyridine and methyl(3-bromopropyl)carbamate were treated in the similar manner to ReferenceExample 102(1) to givemethyl[3-(3-iodo-6-methyl-1H-pyrazolo[3,4-b]pyridin-1-yl)propyl]carbamate[REx(104-3)] as a colorless powder.

APCI-MS m/z: 375 [M+H]⁺.

4)Methyl[3-(3-iodo-6-methyl-1H-pyrazolo[3,4-b]pyridin-1-yl)propyl]carbamateand tri-n-butyltin-ethoxyvinyl were treated in the similar manner toReference Example 102(2) to give methyl[3-(3-acetyl-6-methyl-1H-pyrazolo[3,4-b]pyridin-1-yl)propyl]carbamate[REx(104-4)] as a colorless powder.

APCI-MS m/z: 291 [M+H]⁺.

Reference Example 105

1) To a solution of 1H-pyrrole (5.0 g) in N,N-dimethylformamide (40 mL)was added drop by drop sodium hydride (3.58 g) under ice-cooling, andthen the mixture was stirred at room temperature for 20 minutes. Then,thereto was added dropwise a solution of 1-bromo-3-methoxypropane (2.74g) in N,N-dimethylformamide (2 mL) under ice-cooling, and the mixturewas stirred at room temperature for 2 hours. To the reaction mixture wasadded water under ice-cooling, and then the mixture was extracted withdiethyl ether. The organic layer was washed with saturated saline, driedover sodium sulfate, and then concentrated under reduced pressure. Theresulting residue was purified by silica gel column chromatography(eluent: n-hexane/ethyl acetate=100/1→20/1) to give1-(3-methoxypropyl)-1H-pyrrole [REx(105-1)] (9.07 g) as a colorless oil.2) A solution of 1-(3-methoxypropyl)-1H-pyrrole (3.92 g), ethyl3-dimethylamino-2-(dimethylaminomethyleneamino)acrylate (7.20 g) (ref.Liebigs Ann. Chem. 1980, 344-357) and trifluoroacetic acid (8.33 mL) inacetic acid (32 mL) was stirred at room temperature for 18 hours, andthen heated to reflux for 3 hours. After cooling, the reaction solutionwas concentrated under reduced pressure. To the resulting residue wasadded aqueous saturated sodium hydrogen carbonate solution underice-cooling, and the mixture was extracted with ethyl acetate. Theorganic layer was washed with saturated saline, dried over sodiumsulfate, and then concentrated under reduced pressure. The resultingresidue was purified by silica gel column chromatography (eluent:n-hexane/ethyl acetate=1/1→AcOEt) to give ethyl1-(3-methoxypropyl)-1H-pyrrolo[3,2-c]pyridine-6-carboxylate [REx(105-2)](4.79 g) as a brown oil.

APCI-MS m/z: 263 [M+H]⁺.

3) To a solution of ethyl1-(3-methoxypropyl)-1H-pyrrolo[3,2-c]pyridine-6-carboxylate (2.00 g) indichloromethane (40 mL) was added N-bromosuccinimide (1.49 g) underice-cooling, and the mixture was stirred at room temperature for 3hours. To the reaction solution was added water, and the mixture wasextracted with chloroform. The organic layer was washed with saturatedsaline, dried over sodium sulfate, and then concentrated under reducedpressure. The resulting residue was purified by NH-silica gel columnchromatography (eluent: n-hexane/ethyl acetate=9/1→4/1) to give ethyl3-bromo-1-(3-methoxypropyl)-1H-pyrrolo[3,2-c]pyridine-6-carboxylate[REx(105-3)] (2.12 g) as a yellow oil.

APCI-MS m/z: 341/343 [M+H]⁺.

4) To a solution of ethyl3-bromo-1-(3-methoxypropyl)-1H-pyrrolo[3,2-c]pyridine-6-carboxylate(1.70 g) in 1,4-dioxane (25 mL) were added trimethylboroxine (2.09 mL),cesium carbonate (4.87 g),2-dicyclohexylphosphino-2′,4′,6′-triisopropyl-1,1′-biphenyl (X-Phos)(297 mg) and tris(dibenzylideneacetone)dipalladium (228 mg) under argon,and the mixture was stirred at 110° C. for 15 hours. The reactionsolution was cooled, and then thereto was added water, and the mixturewas extracted with ethyl acetate. The organic layer was washed withsaturated saline, dried over sodium sulfate, and then concentrated underreduced pressure. The resulting residue was purified by silica gelcolumn chromatography (eluent: n-hexane→ethyl acetate) to give ethyl1-(3-methoxypropyl)-3-methyl-1H-pyrrolo[3,2-c]pyridine-6-carboxylate[REx(105-4)] (831 mg) as a yellow oil.

APCI-MS m/z: 277 [M+H]⁺.

5) To a solution of ethyl1-(3-methoxypropyl)-3-methyl-1H-pyrrolo[3,2-c]pyridine-6-carboxylate(100 mg) in chloroform (2 mL) was added meta-chloroperoxybenzoic acid(250 mg) under ice-cooling, and then the mixture was stirred at roomtemperature for 2 hours. The reaction solution was concentrated, andthen the resulting residue was purified by NH-silica gel columnchromatography (eluent: ethyl acetate→ethyl acetate/methanol=10/1) togive ethyl1-(3-methoxypropyl)-3-methyl-1H-pyrrolo[3,2-c]pyridine-6-carboxylate5-oxide [REx(105-5)] (41 mg) as a pale yellow oil.

APCI-MS m/z: 293 [M+H]⁺.

6) A solution of ethyl1-(3-methoxypropyl)-3-methyl-1H-pyrrolo[3,2-c]pyridine-6-carboxylate5-oxide (40 mg) in phosphorus oxychloride (2 mL) was stirred at 100° C.for 1 hour. The reaction solution was concentrated, and the resultingresidue was dissolved in ethyl acetate. It was sequentially washed withaqueous saturated sodium hydrogen carbonate solution and saturatedsaline, and dried over sodium sulfate, and then concentrated underreduced pressure. The resulting residue was purified by silica gelcolumn chromatography (eluent:4-chloro-1-(3-methoxypropyl)-3-methyl-1H-pyrrolo[3,2-c]pyridine-6-carboxylate[REx(105-6)] (23 mg) as a colorless powder.

APCI-MS m/z: 311/313[M+H]⁺.

7) To a solution of ethyl4-chloro-1-(3-methoxypropyl)-3-methyl-1H-pyrrolo[3,2-c]pyridine-6-carboxylate(290 mg) in ethanol (6 mL) was added 2-normal aqueous sodium hydroxidesolution (0.95 mL) under ice-cooling, and the mixture was stirred atroom temperature for 90 minutes. Then, thereto was added 2-normalhydrochloric acid (0.95 mL) under ice-cooling, and then the reactionsolution was concentrated. To a solution of the residue in chloroform (6mL) were added N,O-dimethylhydroxyamine hydrochloride (137 mg),1-ethyl-3-(3-dimethylaminopropyl)carbodiimide hydrochloride (268 mg),1-hydroxybenzotriazole (189 mg) and diisopropylethylamine (325 μL) underice-cooling, and then the mixture was stirred at room temperature for 15hours. To the reaction mixture was added aqueous saturated sodiumhydrogen carbonate solution under ice-cooling, and the mixture wasextracted with chloroform. The organic layer was sequentially washedwith water and saturated saline, dried over sodium sulfate, and thenconcentrated under reduced pressure. The resulting residue was purifiedby silica gel column chromatography (eluent: n-hexane→ethyl acetate) togive4-chloro-N-methoxy-1-(3-methoxypropyl)-N,3-dimethyl-1H-pyrrolo[3,2-c]pyridine-6-carboxamide[REx(105-7)] (277 mg) as a colorless oil.

APCI-MS m/z: 326/328 [M+H]⁺.

8)4-Chloro-N-methoxy-1-(3-methoxypropyl)-N,3-dimethyl-1H-pyrrolo[3,2-c]pyridine-6-carboxamideand methylmagnesium bromide were treated in the similar manner toReference Example 6(5) to give1-[4-chloro-1-(3-methoxypropyl)-3-methyl-1H-pyrrolo[3,2-c]pyridin-6-yl]ethanone[REx(105-8)] as a colorless powder.

APCI-MS m/z: 281/283 [M+H]⁺.

9) To a solution of1-[4-chloro-1-(3-methoxypropyl)-3-methyl-1H-pyrrolo[3,2-c]pyridin-6-yl]ethanone(50 mg) in 1,4-dioxane (2 mL) were added trimethylboroxine (50 μL),cesium carbonate (174 mg),2-dicyclohexylphosphino-2′,4′,6′-triisopropyl-1,1′-biphenyl (X-Phos) (17mg) and tris(dibenzylideneacetone)dipalladium (8 mg) under argon, andthe mixture was stirred at 80° C. for 2 hours. The reaction solution wascooled, and then thereto was added water, and the mixture was extractedwith ethyl acetate. The organic layer was washed with saturated saline,dried over sodium sulfate, and then concentrated under reduced pressure.The resulting residue was purified by silica gel column chromatography(eluent: n-hexane→n-hexane/ethyl acetate=1/1) to give1-[1-(3-methoxypropyl)-3,4-dimethyl-1H-pyrrolo[3,2-c]pyridin-6-yl]ethanone[REx(105-9)] (105 mg) as a colorless oil.

APCI-MS m/z: 261 [M+H]⁺.

Reference Example 106

1) To a solution of ethyl3-bromo-1-(3-methoxypropyl)-1H-pyrrolo[3,2-c]pyridine-6-carboxylate (200mg) in 1,4-dioxane (2 mL) were added trivinylboroxine pyridine complex(141 mg), cesium3-Ethyl-N-methoxy-1-(3-methoxypropyl)-N-methyl-1H-pyrrolo[3,2-c]pyridine-6-carboxamideand methylmagnesium bromide were treated in the similar manner toReference Example 6(5) to give1-[3-ethyl-1-(3-methoxypropyl)-1H-pyrrolo[3,2-c]pyridin-6-yl]ethanone[REx(106-4)] as a pale yellow oil.

APCI-MS m/z: 261 [M+H]⁺.

Reference Example 107

4-Bromo-1-methoxy-2-(4-methoxybutyl)benzene andtri-n-butyltin-1-ethoxyvinyl were treated in the similar manner toReference Example 27(3) to give1-[4-methoxy-3-(4-methoxybutyl)phenyl]ethanone as a yellow oil.

APCI-MS m/z: 237 [M+H]⁺.

Reference Examples 108 to 112

Compounds of Reference Examples 103 to 107 were treated in the similarmanner to Reference Example 6-(6) to give the following compounds.

TABLE 90 Ref. EX. No. Structural formula a b c d e 108

317.3861 O 318 [M + H]⁺ 109

331.4127 O 332 [M + H]⁺ 110

301.4265 O 302 [M + H]⁺ 111

301.4265 O 302 [M + H]⁺ 112

277.4018 O 278 [M + H]⁺

Ref Ex. No.: Reference Example Number d: MS Results APCI

a: Salt e: Ion species

b: Molecular weight O: Oil

c: Properties

Reference Example 113

1) 5-Bromo-6-chloronicotinic acid and N,O-dimethylhydroxyaminehydrochloride were treated in the similar manner to Reference Example7(5), and then the resulting compound and methylmagnesium bromide weretreated in the similar manner to Reference Example 7(6) to give1-(5-bromo-6-chloropyridin-3-yl)ethanone [REx(113-1)] as a colorlesspowder.

APCI-MS m/z: 234/236 [M+H]⁺.

2) 1-(5-Bromo-6-chloropyridin-3-yl)ethanone and cyclopropylamine weretreated in the similar manner to Reference Example 6(6) to giveN-[1-(5-bromo-6-chloropyridin-3-yl)ethyl]cyclopropylamine [REx(113-2)]as a pale yellow oil.

APCI-MS m/z: 275/277 [M+H]⁺.

3) To a solution ofN-[1-(5-bromo-6-chloropyridin-3-yl)ethyl]cyclopropylamine (2.47 g) inethyl acetate (15 mL)-tetrahydrofuran (15 mL)-water (15 mL) were addedsodium hydrogen carbonate (3.78 g) and di-tert-butyl dicarbonate (3.94g), and the mixture was stirred at room temperature for 41 hours. To thereaction solution was added water, and then the mixture was extractedwith ethyl acetate. The organic layer was washed with saturated saline,dried over sodium sulfate, and then concentrated under reduced pressure.The resulting residue was purified by silica gel column chromatography(eluent: n-hexane/ethyl acetate=4/1→4/1) to give tert-butyl[1-(5-bromo-6-chloropyridin-3-yl)ethyl]cyclopropylcarbamate [REx(113-3)](2.6 g) as a pale yellow oil.4) To a solution of t-butyl[1-(5-bromo-6-chloropyridin-3-yl)ethyl]cyclopropylcarbamate (530 mg) inN,N-dimethylformamide (8 mL) were added methyl prop-2-yn-1-ylcarbamate(384 mg), triethylamine (1.96 mL),dichlorobis(triphenylphosphine)palladium (II) (69 mg) and copper (I)iodide (40 mg), and the mixture was stirred at 60° C. for 2 hours. Thereaction solution was cooled, and then diluted with ethyl acetate, andan insoluble was filtered off. The filtrate was sequentially washed withwater and saturated saline, dried over sodium sulfate, and thenconcentrated under reduced pressure. The resulting residue was purifiedby silica gel column chromatography (eluent: n-hexane/ethylacetate=4/1→1/1) to give methyl[3-(5-{1-[(tert-butoxycarbonyl)(cyclopropyl)amino]ethyl}-2-chloropyridin-3-yl)prop-2-yn-1-yl]carbamate[REx(113-4)] (362 mg) as a pale yellow oil.

APCI-MS m/z: 408/410 [M+H]⁺.

5) Methyl[3-(5-{1-[(tert-butoxycarbonyl)(cyclopropyl)amino]ethyl}-2-chloropyridin-3-yl)prop-2-yn-1-yl]carbamatewas reduced in the similar manner to Example 296(5) to give methyl[3-(5-{1-[(tert-butoxycarbonyl)(cyclopropyl)amino]ethyl}-2-chloropyridin-3-yl)propyl]carbamate [REx(113-5)] as a pale yellow oil.

APCI-MS m/z: 412/414 [M+H]⁺.

6) To a solution of methyl[3-(5-{1-[(tert-butoxycarbonyl)(cyclopropyl)amino]ethyl}-2-chloropyridin-3-yl)propyl]carbamate(380 mg) in dimethoxyethane (8 mL) were added vinyl boronic acid pinacolester (235 μL), 2M sodium carbonate (1.38 mL) anddichlorobis(triphenylphosphine)palladium (II) (65 mg), and the mixturewas stirred at 85° C. for 17 hours. The reaction solution was cooled,and then an insoluble was filtered off through Celite, and to thefiltrate was added water, and the mixture was extracted with ethylacetate. The organic layer was washed with saturated saline, dried oversodium sulfate, and then concentrated under reduced pressure. Theresulting residue was purified by silica gel column chromatography(eluent: n-hexane/ethyl acetate=3/1→4/4) to give methyl[3-(5-{1-[(tert-butoxycarbonyl)(cyclopropyl)amino]ethyl}-2-vinylpyridin-3-yl)propyl]carbamate [REx(113-6)] (282 mg) as a pale yellow oil.

APCI-MS m/z: 404[M+H]⁺.

7) To a solution of methyl[3(5-{1-[(tert-butoxycarbonyl)(cyclopropyl)amino]ethyl}-2-vinylpyridin-3-yl)propyl]carbamate(280 mg) in methanol (10 mL) was added 10% palladium on carbon (140 mg),and the mixture was stirred under hydrogen for 2 hours. An insoluble wasfiltered off, and then the filtrate was concentrated under reducedpressure. The resulting residue was purified by silica gel columnchromatography (eluent: chloroform→chloroform/methanol=4/1) to givemethyl[3-(5-{1-[(tert-butoxycarbonyl)(cyclopropyl)amino]-ethyl}-2-ethylpyridin-3-yl)propyl]carbamate[REx(113-7)] (210 mg) as a pale yellow oil.

APCI-MS m/z: 406 [M+H]⁺.

8) To a solution of methyl[3-(5-{1-[(tert-butoxycarbonyl)(cyclopropyl)amino]ethyl}-2-ethylpyridin-3-yl)propyl]carbamate(204 mg) in dichloromethane (1.5 mL) was added trifluoroacetic acid (1.5mL) under ice-cooling, and the mixture was stirred at room temperaturefor 1 hour. The reaction solution was poured into ice-cooled aqueoussaturated sodium hydrogen carbonate solution, and extracted withchloroform. The organic layer was washed with saturated saline, driedover sodium sulfate, and then concentrated under reduced pressure. Theresulting residue was purified by NH-silica gel column chromatography(eluent: chloroform→chloroform/methanol=20/1) to give methyl(3-{5-[1-(cyclopropylamino)ethyl]-2-ethylpyridin-3-yl}propyl)carbamate[REx(113-8)] (142 mg) as a pale yellow oil.

APCI-MS m/z: 306 [M+H]⁺.

Reference Example 114

To a solution of (−)-α-pinene (3.64 mL) in tetrahydrofuran (5 mL) wasadded dropwise borane-dimethyl sulfide complex (1.09 mL) underice-cooling, and the mixture was stirred at room temperature for 15hours. To the reaction solution was added dropwise a solution of methylprop-2-yn-1-ylcarbamate (1.0 g) in tetrahydrofuran (3 mL) underice-cooling, and then the mixture was stirred at room temperature for 17hours. To the reaction solution was added dropwise acetaldehyde (5 mL)under ice-cooling, and the mixture was stirred at room temperature for 3hours. The reaction solution was concentrated under reduced pressure,and the resulting residue was dissolved in diethyl ether (15 mL). To thesolution was added pinacol (1.56 g), and the mixture was stirred at roomtemperature for 2 hours. The reaction solution was washed with water,and then dried over magnesium sulfate and concentrated under reducedpressure. The resulting residue was purified by silica gel columnchromatography (eluent: n-hexane/ethyl acetate=10/1→3/2) to give methyl[(2E)-3-(4,4,5,5-tetramethyl-1,3,2-dioxaboran-2-yl)prop-2-en-1-yl]carbamate(1.18 g) as a pale yellow oil.

APCI-MS m/z: 242 [M+H]⁺.

Test Example Inhibitory Activity Against Human Renin

A substrate of synthetic peptide (Nma-KHPFH LVIHK(Dnp)-NH₂) and testcompound were mixed, and fluorescence intensity was assayed using afluorophotometer before starting an enzymatic reaction (excitingwavelength: 340 nm, measuring wavelength: 460 nm). Recombinant humanrenin was added and the mixture was incubated at 37° C. for 1 hour, andthe fluorescence intensity was measured after the reaction using afluorophotometer (exciting wavelength: 340 nm, measuring wavelength: 460nm). Renin activity was evaluated on the ground of fluorescenceintensity which was obtained by deduction of the intensity before thereaction from the intensity after the reaction, and 50% inhibitoryconcentration (IC50) was calculated from renin activities under theexistence of various concentration of the test compound. Examplecompounds herein showed the following values.

Test Result 1

TABLE 91 Example IC50 (nM) 6 13 7 13 8 490 9 7.3 10 1.2 12 73 17 210 1844 19 10 21 8.4 22 270 24 240 25 19 26 320 27 300 28 1.4 29 5.9 30 30 31580 35 6.8 36 0.5 37 6.6 38 3.7 39 1.4 40 14 41 2.3 42 1.5 43 1.6 44 3045 8.5 46 17 49 89 52 4.2 53 72 54 31 55 1.9 56 35 57 0.4 58 16 59 2.160 7 61 14 62 12 63 800 64 0.9 65 6.4 66 2.4 67 1.5 68 140 69 21 70 0.671 21 75 78 76 37 78 1 79 1.1 80 8.4 81 25 82 16 83 330 85 11 86 16 87 788 52 89 3.9 90 71 91 15 92 62 93 41 94 120 97 0.7 98 4.4 99 7.3 100 41101 29 102 290 103 2.4 104 89

Test Result 2

TABLE 92 Example IC50 (nM) 105 8.1 106 530 109 500 110 67 113 540 1144.1 115 87 116 16 122 19 123 42 124 3.2 125 12 126 6.6 127 15 128 3.3129 42 132 16 133 20 134 250 135 11 136 33 137 6.3 138 12 139 2.5 140 10141 4 142 17 143 58 144 4.8 145 63 146 28 147 16 149 13 150 8.7 151 34152 1.2 153 46 268 3.6 269 6.6 270 0.4 271 3.7 276 2.5 280 0.9 281 6.7284 0.5 285 6.1 286 3 287 35

Test Result 3

TABLE 93 Example IC50 (nM) 299 1.6 301 3.3 304 1.3 305 12 306 230

INDUSTRIAL APPLICABILITY

The compound [I] of the present invention or a pharmaceuticallyacceptable salt thereof has renin inhibitory activity and may be usefulfor treatment and/or prophylaxis of hypertension, cardiac failure,diabetic nephropathy and the like. Furthermore, the compound [II] isuseful as a synthetic intermediate for preparing the compound [I].

1. A compound of the formula [I];

wherein R¹ is a cycloalkyl group or a non-substituted alkyl group; R²²is 1) an optionally substituted aryl group, 2) an optionally substitutedtetrahydronaphthyl group, or 3) an optionally substituted pyridyl group;R is a lower alkyl group; T is a carbonyl group; Z is —O—; R³, R⁴, R⁵and R⁶ are the same or different, a hydrogen atom, an optionallysubstituted carbamoyl group or an optionally substituted alkyl group; ora pharmaceutically acceptable salt thereof.
 2. The compound according toclaim 1, wherein R²² is any group selected from; a phenyl groupoptionally substituted with the same or different, one to four group(s)selected from a phenyl lower alkoxy group, a halogen atom, a lower alkylgroup, a lower alkyl group substituted with a lower alkoxy group, alower alkyl group substituted with a lower alkoxycarbonylamino group, alower alkoxy group substituted with a lower alkoxy group, an aryl groupsubstituted with a lower alkoxy group, a heterocyclic group, a cyanogroup and a lower alkoxy group, a naphthyl group optionally substitutedwith the same or different, one to six group(s) selected from atrihalogeno lower alkoxy group, a lower alkanoylamino lower alkoxygroup, a halogen atom, a lower alkyl group substituted with a loweralkoxy group, a lower alkyl group substituted with a loweralkoxycarbonylamino group, a lower alkyl group, a lower alkoxy groupsubstituted with a lower alkoxy group, an aryl group, an aryl groupsubstituted with a lower alkoxy group, a heterocyclic group, a cyanogroup and a lower alkoxy group, a tetrahydronaphthyl group optionallysubstituted with the same or different, one to six group(s) selectedfrom a halogen atom, a lower alkyl group substituted with a lower alkoxygroup, a lower alkyl group substituted with a lower alkoxycarbonylaminogroup, a lower alkyl group, a lower alkoxy group substituted with alower alkoxy group, an aryl group substituted with a lower alkoxy group,a heterocyclic group, a cyano group and a lower alkoxy group, and apyridyl group optionally substituted with the same or different, one tofour group(s) selected from a halogen atom, a lower alkyl groupsubstituted with a lower alkoxy group, a lower alkyl group substitutedwith a lower alkoxycarbonylamino group, a lower alkyl group, a loweralkoxy group substituted with a lower alkoxy group, an aryl groupsubstituted with a lower alkoxy group, a heterocyclic group, a cyanogroup and a lower alkoxy group, or a pharmaceutically acceptable saltthereof.
 3. The compound according to claim 1, wherein R²² is selectedfrom 1) an optionally substituted naphthylidinyl group, and 2) anoptionally substituted pyridyl group, or a pharmaceutically acceptablesalt thereof.
 4. The compound according to claim 1, which is shown bythe formula I^(c1):

wherein R^(b) is lower alkyl, R^(b1) is cycloalkyl or alkyl, the ring Bis selected from 1) an aryl group 2) a tetrahydronaphthyl group, and 3)a pyridyl group, R^(b21) to R^(b23) are the same of different, and agroup selected from 1) hydrogen, 2) halogen, 3) alkyl optionallysubstituted with a group selected from halogen, alkoxy andalkoxycarbonylamino, 4) alkoxy optionally substituted with a groupselected from alkoxy and alkoxycarbonylamino, 5) cyano, 6) carbamoyloptionally substituted with alkyl and 7) oxo, or a pharmaceuticallyacceptable salt thereof.
 5. The compound according to claim 4, which isshown by the formula I^(c2):

wherein R^(b) is lower alkyl, R^(b1) is cycloalkyl or alkyl, the ring Bis selected from 1) an aryl group 2) a tetrahydronaphthyl group, and 3)a pyridyl group, R^(b21) to R^(b23) are the same of different, and agroup selected from 1) hydrogen, 2) halogen, 3) alkyl optionallysubstituted with a group selected from halogen, alkoxy andalkoxycarbonylamino, 4) alkoxy optionally substituted with a groupselected from alkoxy and alkoxycarbonylamino, 5) cyano, 6) carbamoyloptionally substituted with alkyl and 7) oxo, or a pharmaceuticallyacceptable salt thereof.
 6. The compound according to claim 4 or 5,wherein the ring B is selected from a pyridyl group, or apharmaceutically acceptable salt thereof.
 7. The compound according toclaim 4, wherein R^(b1) is a cycloalkyl group, or a pharmaceuticallyacceptable salt thereof.
 8. The compound according to claim 7, whereinR^(b1) is a cyclopropyl group, or a pharmaceutically acceptable saltthereof.
 9. The compound according to claim 4, wherein R^(b21) is agroup selected from alkyl optionally substituted with alkoxy andalkoxycarbonylamino, and alkoxy optionally substituted with alkoxy andalkoxycarbamoyl, or a pharmaceutically acceptable salt thereof.
 10. Apharmaceutical composition comprising the compound according to claim 1.11. A pharmaceutical composition for use in the treatment and/orprophylaxis of hypertension, cardiac failure, diabetic nephropathy andthe like, comprising the compound according to claim 1, or apharmaceutically acceptable salt thereof.
 12. A method for the treatmentof hypertension, cardiac failure, diabetic nephropathy and the like,comprising administration of the compound according to claim 1 or thepharmaceutically acceptable salt thereof.
 13. A compound of the formula[II]:

wherein R¹ is a cycloalkyl group or a non-substituted alkyl group; R²²is 1) an optionally substituted aryl group, 2) an optionally substitutedtetrahydronaphthyl group, or 3) an optionally substituted pyridyl group;R is a lower alkyl group; T is a carbonyl group; Z¹ is —O—; R³, R⁴, R⁵and R⁶ are the same or different, a hydrogen atom, an optionallysubstituted carbamoyl group or an optionally substituted alkyl group; P¹is a protecting group and P² is a protecting group; or a salt thereof.